查询词典 cytometer

The present study is involved in the expression of vitronectin , a ligand of integrin, on human spermatozoa and its role in fertilization; the expression of integrin α〓、α〓、β〓 sbunits on human and mouse oocytes and their relationship with maturation of oocytes; the reorganization of cytoskeletal protein, actin, of mouse oocytes mediated with RGD peptide binding with integrins; the roles and mechanisms of integrins in activation of mouse oocytes as trasmembrane signaling receptors, using the methos of cell culture, immunohistochemistry, flow cytometer, laser confocal microscope.

本课题采用细胞培养、免疫组化、流式细胞术、激光共聚焦显微技术进行以下四个方面的研究：整合素配体玻连蛋白在人精子的表达及其与精子功能状态及受精的关系：整合素〓在人、小鼠卵的表达及其与卵的成熟程度的关系：整合素介导小鼠卵内actin骨架蛋白的重组；整合素介导卵激活的早、晚期事件，包括卵细胞内钙离子浓度的变化及孤雌发育胚的形成，并探讨其机制。

Using Flow cytometer,TriTest reagent and Trucount tube(TriTEST/TruCOUNT)to assess absolute counts of CD 3,CD 4 and CD 8T lymphocyte subsets and CD 4/CD 8 ratio and observe T lymphocyte subsets distribution in sex,age,ethnics in the 110 healthy adults(Age∶16-56)in Guangxi.

用流式细胞仪(FACSCalibur，FCS)、三色免疫荧光试剂和绝对计数管(TnTEST/Tru COUNT)，检测 110名广西健康成年人(16～ 5 6岁)静脉全血CD3 、CD4、CD8T淋巴细胞绝对数值和CD4/CD8比值，并观察T淋巴细胞亚群分类计数在性别、年龄和民族上的差别。

Methods MSCs were obtained from tibias by density gradient centrifugation and were tested the rate of adhesion. The configuration was observed under phase-contrast microscope consecutively and the cell growth was measured by MTT method. Cell cycle and surface antigens were detected through flow cytometer.

测定其接种贴壁率，在倒置显微镜下连续观察细胞的形态变化，利用甲基噻唑基四唑（methyl thiazdyl tetrazolium， MTT）法测定 MSCs 生长曲线，并应用流式细胞术测定细胞表面标记物。

This article is to detect the effect of rabbit sera on cell circle and DNA of Shope carcinoma cells with Flow cytometer.

用流式细胞仪检测兔血清对shope肿细胞的细胞周期及细胞DNA的影响。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组：①正常对照组，②缺血再灌注组（1R组），③缺血预处理组，观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化；采用MTT法检测线粒体的功能；利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况；采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况，结合自动图像分析系统对其结果进行定量分析；在光镜下观察细胞的形态学改变。

Nucleus changes in apoptotic cells were investigated by using Hoechst 33258 staining and photofluorography. Apoptotic rate was evaluated by propidium iodide staining and flow cytometer.

应用Hoechst 33258染色法观察凋亡细胞的形态学改变；PI染色流式细胞仪检测细胞凋亡率；Western印迹法检测Bcl-2蛋白的表达。

Methods: According to their urinary albumin excretion rate, Diabetes mellitus patients with micrangium disease were further divided into three subgroups: normo-albuminuria、micro-albuminuria and clinical proteinuria; The expression of platelet membrane α-glucoprotein (CD62p) and cytolysosome protein (CD63)、platelet aggregation function and mean platelet volume was determined with flow cytometer、blood aggregation analyzer、automated blood cell counter (MEK-6318K) respectively, and compared with 30 normal controls.

对糖尿病微血管病变患者按尿清蛋白排泄率分为正常清蛋白尿组、微量清蛋白尿组、临床清蛋白尿组三组，分别用流式细胞术、血液聚集仪、全自动血细胞分析仪测定血小板内α-颗粒膜蛋白(CD62p)、溶酶体蛋白(CD63)的表达及血小板聚集功能和平均体积，并与30例正常对照组比较。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐