查询词典 cytometer

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

Methods Human prostate cancer PC-3 cell, breast cancer ER-75-30 cell and lung cancer A549 cell were cultured in RPMI medium 1640.Light microscope,transmissional electron microscope, flow cytometer and immunohistochemical method were used to observe apoptosis morphological changes of three kinds of tumor cell lines, time and dose effect relationship and to detect expression of bcl-2 and bax.

方法人前列腺癌 PC-3细胞株、乳腺癌 ER-75-30细胞株和非小细胞肺癌 A549细胞株经培养后，采用光镜、电镜、流式细胞仪和免疫组化方法研究 188Re诱导 3种肿瘤细胞凋亡的形态学变化、时间剂量效应关系、细胞周期依赖性以及凋亡相关基因 bcl-2和 bax在其中的表达情况。

METHODS: The drug sensitivity test was performed with trypan blue and the apoptotic cells were measured with flow cytometer.

采用台盼蓝排斥法进行药物敏感试验，流式细胞仪测定凋亡细胞含量。

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态，测定其生长动力学，采用流式细胞仪及免疫细胞化学方法检测其表型，应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein， ac-LDL)的摄取功能，Matrigel上培养并检测其形成血管能力，染色体核型分析其遗传稳定性，裸鼠实验、软琼脂实验观察致瘤性。

The study ~s conducted to elucidate the mechanism by which the expression of adhesion molecules on human endothelial cells ~s up-regulatecL Methods Human endothelial cells derived fium umbilical venisQ-IUVEC were coincubated in viny whh native human sennn albtuiiin, HSA modified v~ith advanced glycation end products or RPMI-1640 culture medium. The expression of adhesion molecule intereellular adhesion molecule-l(7ICAM-l), vascular cell adhesion molecule-l and E-selectin was determined by irnrnunofluorescen staining and flow cytometer analysis.

分离正常人脐静脉内皮细胞，将晚期糖基化终产物修饰的人血清白蛋白、人血清白蛋白以及RPMI-1640完全培养基与HUVEC在体外共同培养，用荧光单克隆抗体染色，流式细胞仪定量检测内皮细胞表面细胞间粘附分子-1（ICAM-1）、血管细胞间粘附分子-1（VCAM-1）、E-选择素的表达。

65 coxes with extensive and medium acute cerebral infarction were divided into two groups, the treatment group (33 cases) were given Clearing Heat and Toxin drug and Xingnaojing Injection, the control group were given CDPC Injection in addition toconventional treatment. The level of CD62P TNF-α, IL-6 before and after treatment were measured by flow cytometer and ECL method, and compared with that of 30 healthy people. Meanwhile the clinical curative effect and the neurological impairment scale were observed. Results: The level of CD62P, TNF-α, IL-6 in ACI group were higher than that in healthy group.

将65例大、中等面积ACI患者随机分为两组，治疗组33例采用西医常规治疗加清热解毒中药和醒脑静注射液，对照组32例给予西医常治疗加胞二磷胆碱注射液；测定两组治疗前后血小板CD62P阳性表达率与TNF-α、IL-6水平，并与30例健康人组作对照相关分析，同时观察临床疗效及神经功能缺损评分情况。

Cerebral cortexes were removed and then dissociated, measured with flow cytometer analyze cell apoptosis, the morphological changes were examined by electron microscope.(2) Measured cell apoptosis of cerebral cortexes with TUNEL.(3) detected expressions of Bcl-2, Bax and Caspase-3 proteins with the technique of immunohistochemistry.

1取大脑皮层细胞制成细胞悬液，流式细胞仪分析细胞凋亡改变，电镜观察细胞超微结构的变化；(2)用原位缺口末端标记法检测凋亡细胞百分率；(3)免疫组化法检测Bcl-2、Bax和Caspase-3蛋白表达。

However, flow cytometers suffer from their intrinsic inability to tolerate large particles that associate with the isolated nuclei. A plant nuclei suspension can often contain a high level of crystalline calcium oxalate that blocks the fluidics system of the flow cytometer.

但是植物的细胞核悬浮液中常含有草酸钙结晶，样品中过大的杂质颗粒常会堵塞流式细胞仪吸取样品的管子。

For evaluation of the hybridoma cells, immumofluorescence method with flow cytometer were used.

人骨髓间充质干细胞的特异性细胞表面抗原单克隆抗体可望在MCSs细胞的提纯和体外富集，特异性骨病的诊断等方面有广阔的应用前景。

Methods: The level of TRAIL, the proliferation index and apoptosis rate were detected by flow cytometer analysis in 23 case of hemangioma at proliferating phase, 20 cases of hemangioma at involuting phase, and 15 eases of normal skin.

应用流式细胞术检测23例增生期、20例消退期血管瘤和15例正常皮肤组织中TRAIL蛋白的相对含量，细胞增殖指数和细胞凋亡率。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐