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In order to efficiently collect information and tissue samples of pangolins rescued by residents around Taiwan, we assisted in designing and executing a wildlife rescue system for the Formosan Pangolin (Manis pentadactyla pentadactyla). Data and samples collected from this system were analyzed to evaluate its efficiency in providing information for pangolin conservation. This study also examined genetic variability and structure across pangolin populations in Taiwan by using mtDNA sequences of left domain of control region and the partial cytochrome-b gene. Implication of the results from these analyses in pangolin management and conservation was discussed.

本研究藉由协助台湾穿山甲（Manis pentadactyla pentadactyla）救伤流程与通报系统的规划和运作，收集台湾各地因救伤而进入本流程的台湾穿山甲相关资讯与组织样本，进行分析，以评估穿山甲救伤通报系统的成效和保育上的应用，包括以粒线体DNA中的细胞色素b与控制区片段为分子标记，检视台湾穿山甲岛内遗传结构与遗传变异程度，并从族群遗传的角度提供未来保育策略上的建议。

According to the alignment of Blastn and Blastx, one differentially exp ressed fragment, A7, was 97% identical in nucleotides to the 26S ribosomal RNA gene of Photinia fraseri and 91% in amino acids to cytochrome P450 monooxygenase in maize, respectively. Another differentially exp ressed fragment, A16, was 85% in nucleotides and 93% in amino acids identical to alpha-expansin 2 in Triphysaria versicolor.

核酸和氨基酸比对分析中同源性最高的是片段A7和A16.A7的核苷酸序列同源于红叶石楠Photinia fraseri 26S核糖体RNA (97%)、蛋白同源于玉米的细胞色素P450单加氧酶(91%)， A16的核苷酸(85%)及蛋白(93%)同源于直果草属 Triphysaria versicolor 的α-expansin 2基因。

Phylogenetic relationships of brown frogs with 24 chromosomes from Far East Russia and Hokkaido assessed by mitochondrial cytochrome b gene sequences ( Rana : Ranidae).

亲缘关系的布朗与24个染色体青蛙从俄罗斯远东地区和北海道进行评估，线粒体细胞色素b基因序列。

IntroductionSmooth surfaced endoplasmic reticula contain plenty of cyto-chromes, which are mainly cytochrome P450 ( CYP450 ) and b5 and are very important in the process of the biotransformation of exogenetical fat - soluble compounds and the metabolism of some endogenetical matrices, such as bile acid forming^ bile pigment metabolism and steroid synthesis.

CYP450是50年代中期Williams和Klingeberg发现、60年代初木村和佐藤验证的一类b族细胞色素，由于还原型细胞色素与一氧化碳结合后在光谱的450nm处具有最高吸收峰，因而命名为CYP450以别与其它b族细胞色素，P是英文Peak的缩写。

Twenty-four Sparid cytochrome b sequences belonging to 10 genus, 12 species were downloaded from the molecular biology database NCBI.

从NCBI分子生物学数据库下载鲷科鱼类10属12种共24条 Cyt b 序列，通过序列比对最终获得1 070 bp的共有序列。

In the study, a set of near isogenic lines of CMS-S, S-Mo17Rf3Rf3 and S-Mo17rf3rf3, were adopted, and TdT-mediated dUTP nick-end labeling, in situ immunolocalization of cytochrome c and DNA ladder assay were employed to study the programmed cell death process on cytology and DNA level. The microscope observation evidenced that tapetal cells of fertile anthers kept intact until microspores, and then were disintegrated on later developmental stage.

S型细胞质雄性不育系的一对近等基因系S-Mo17Rf3Rf3 和S-Mo17rf3rf3为材料，采用TdT介导的dUTP DNA末端标记、细胞色素C免疫原位杂交和DNA寡聚核小体片段电泳等方法，分别在细胞学水平和DNA水平上研究了玉米CMS-S小孢子败育的细胞程序性死亡过程。

Using the cationic detergent benzyldimethyl -alkylammonium chloride instead of cytochrome C in spreading procedure, we have observed the binding site for EcoR Ⅰ on linear pBR322 DNA molecule in the presence of gluteraldehyde. The diameter of the bound EcoR Ⅰ molecules are 15nm which may be the tetrameric EcoR Ⅰ particles.

用低分子量阳离子去垢剂苯二甲基苄基氯化胺取代经典展层技术中的细胞色素C，汲取前人经验，建立了无蛋白展层技术，适用于蛋白质--DNA相互作用的电镜研究。

Peptide mapping for a tryptic digest of horse cytochrome c was performed by the same method.

对Met-脑啡肽和Leu-脑啡肽的混合物进行了分析，用CZE/MS/MS方法验证了各自的序列。

Terminal oxidative inhibitors, such as KCN, Salicylhydroxamic acid , antimycin A and NaN〓, can cut off electron transport chains of cytochrome pathway and alternative pathway, marked decreased BADH protein levels. Oxidative phosphorylation uncoupler 2, 4-dinitrophenol had a similar negative effect on BADH gene expression, and exogenous ATP and ADP increased the BADH levels. These results, in conjunction with the terminal oxidation results, indicated that ATP could be a critical component in BADH gene expression.

2.2 末端氧化抑制剂KCN、SHAM、抗霉素A和NaN〓能打断呼吸链，阻断电子传递，使BADH蛋白的表达量明显降低，说明末端氧化同样参与BADH基因表达的调节：末端氧化影响基因表达的实质就是ATP的影响，线粒体磷酸化解偶联剂DNP明显降低BADH蛋白的含量，而外源的ATP和ADP对BADH基因表达的正效应作用最大。

AIM: To study the effects of ursolic acid on human MCF7 cell apoptosis and the mechanism probably involved.

目的：探讨中药成分熊果酸（ursolic acid， UA）诱导MCF7人乳腺癌细胞的凋亡作用，并通过UA作用后的MCF7细胞Bax， Bcl2， cytochrome c蛋白表达的变化，探讨其诱导MCF7细胞凋亡的分子机制。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。