查询词典 cyto-architecture

Keywords: Male sterile line; Alfalfa ; backcross; tissue culture; cyto-morphology

中文关键词：雄性不育系；苜蓿；回交；组织培养；细胞形态学

Based on results in this study and our previous findings, we believe that differences of ATPase activity in cells of aloe leaf Chlorenchyma reflect the differences of physiological status and function of aloe leaves, regulating and/or enhancing ATPase activity is an important cyto-physiological mechanism of Si-alleviated effect on salt injury to aloe.

叶同化组织细胞中ATPase活性的差异性反映着芦荟叶生理状态和功能的差异性，可溶性Si调节或增强盐胁迫下芦荟ATPase活性是Si缓解芦荟盐胁迫伤害效应的重要细胞生理机制。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Changes of P53, Caspase 3 and cytochrome C were detected by Western blotting.

药物对P53、Caspase 3，Cyto C等表达的影响检测采用免疫印迹法。

A new hexaploid cyto-type of Coix aquatica was identified and its origin was analyzed.

鉴定了一种新的六倍体细胞类型的水生薏苡，并分析研究了它的起源。

The expression of proliferating cell nuclear antigen in each group was detected by immuno-histochemistry method. The cell cycle distribution was measured by flow cyto- metic analysis.

MTT法及免疫组织化学染色增生细胞核抗原法检测细胞生长活性，流式细胞仪测定细胞周期时相变化。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系，应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后，流式细胞仪技术检测CCR1和CCR2的表达，ELISA法检测细胞培养上清液内白介素-8的浓度；体内实验选用C57BL/6J小鼠，腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg)，LPS刺激4 h后收集BALF和肺脏标本，计数BALF内细胞总数，应用BCA法检测BALF内蛋白浓度，Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

By in vitro cell culture, we testified that SCRN could inhibit the activity of HSV-1 effectively, it also delayed the appearance of cyto- pathic effect .

体外细胞培养证明SCRN能有效地抑制HSV-1的活性，减轻细胞病变程度，延缓CPE出现时间。

The characteristic absorption spectrums of intracellular hemoglobin were detected and analyzed by ultraviolet spectrum scanning technique. The investigation indicated that complicated physiology regulates and controls process, such as red blood cell membrane elasticity deformation, Cyto-membrane liquid crystal state phase transition, the change of hemoglobin configuration, membrane cytoskeleton protein network reassembling or despiralization, and membrane surface electric charge density reapportion were directly influenced by the gentle changes of pH valve in autologous plasma environment. Further, the remarkable diversities of erythrocyte carrying-oxygen ability were appeared in the different pH conditions.

实验研究表明，红细胞弹性形变、细胞膜液晶态相变，胞内血红蛋白分子构型改变，膜骨架网络重新组装或解旋、以及膜表面电荷密度重新分配等复杂的生理调控过程与自体血浆环境中pH值的轻微波动直接相关，可能造成了不同pH条件中活性红细胞携氧能力显著不同。

For "Allstar", cyto-histological observations suggested that adventitous shoots originated from epidermal or subepidermal cells near vascular tissue.

细胞组织学观察表明，对于草莓品种全明星，芽原基多发生于距离维管束较近的表皮或亚表皮细胞处。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。