查询词典 cycle method

Research on characteristics of MCFC-GT hybrid system based on total energy concept This paper discusses the working performance of MCFC-GT hybrid system at diffirent work conditions. The results show that the hybrid system is high efficiency but the change range of work conditions is narrow. The work condition can be extended by a variable-speed gas turbine. However, this method of operation weakens the action of bottoming cycle.

2基于总能概念的MCFC-GT混合动力系统特性分析本文讨论了MCFC-GT混合动力系统不同工况下的工作性能，结果表明：MCFC-GT混合动力系统具有较高的发电效率，但系统的工况变化范围较小，可通过调节燃气轮机的转速来改善系统的变工况性能。

In order to design lighter and faster hydraulic excavator, a rigid-flexible hybrid modeling method based on rigid multi-body dynamics and finite element method was put forward to study the dynamic characteristic of hydraulic excavator in its whole working cycle. The working equipment with characteristic of time-varying structure was taken as research object, and radial acceleration was computed as tags of key load.

为适应液压挖掘机高速、轻量化的要求，以具有时变固频特性的液压挖掘机工作装置为研究对象，提出了以角加速度判断时变结构的关键工况，利用刚性多体动力学和有限元方法对液压挖掘机在整个作业循环中的动力学特性进行建模和分析。

The shear stress of the critical damage plane is determined by the method of multiaxial cycle counting method.

结果表明，临界面法更符合纤绳节点板多轴非比例加载的实际情况，因而可以给出更加合理的结果。

Present multiaxial fatigue life analysis method are discussed, and show that equivalent stress-strain approach based on uniaxial fatigue life analysis method can not predict low-cycle fatigue life under nonproportional cyclic loading.

对现有的多轴疲劳寿命分析方法进行了讨论，论证了以单轴疲劳寿命分析方法为基础的等效应变法不能预测多轴非比例加载下的低周疲劳寿命。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

A factor of friction power is considered during fatigue, and a new model for predicting fretting fatigue life under low cycle load is presented based on a nominal stress method. For dovetail joint, the problem of elastic contact is solved using the finite element method.

在低周疲劳寿命预测方法中，通过引人摩擦功因子，考虑微动作用对构件疲劳寿命的影响，对传统的名义应力法进行改进，以用于构件的低周疲劳寿命预测。

In common use the calculating model of the biggest fall consistency only snits single point polluting source ,as for the multiple points using Ganssian normal model directly calculating practical method of the biggest consistency of comnibearing ground the method when programming and counting,it adopts style of distance step doulbe cycle a.

常用的最大落地浓度预测模式仅适用于单个点污染源，本文对多个点、面源排放提出了用高斯正态模式直接预测全方位地面最大浓度的实用方法。

We re-estimate, compare and analyze China's surplus rural labor by means of labor-hour conversion method and standard structural method. Taking economic cycle fluctuation into account, we also investigate the essential reasons for the coexistence of migrant workers' shortage and agricultural labor's surplus.

通过工日折算法、标准结构法对全国和各省份农村剩余劳动力进行了重新估算和比较分析；并结合经济周期波动，剖析了&民工荒&与大量农村剩余劳动力并存的原因。

In the light of the shortcomings of the traditional method for predicting the fatigue life of coiled tubing, a new method for the reliability analysis of coiled tubing is offered, that is, normalizing the fatigue test data of coiled tubing with different materials and stress states by introducing a compound random variable X=NS2 based on low cycle fatigue curves.

预测连续油管疲劳寿命传统方法是将许多试样在不同应力水平的循环载荷作用下进行试验直至失效，从而作出S－N曲线，供工程参考，这样不仅费用高，且模拟实际应力条件也较困难。为此，根据低周疲劳曲线，通过引入复合随机变量X=NS2，对不同材料和应力水平下的连续油管疲劳实验数据进行了归一化处理。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失