查询词典 cycle method

A combined power cycle with LNG cold energy recovery consisting of Rankine cycle using ammonia-water as working fluid, power cycle of combustion gas, and LNG cycle, was presented.

提出了以氨水为工质的朗肯循环、燃气动力循环和液化天然气循环组成的混合动力循环系统，用于液化天然气冷能回收。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

When the Roman Church altered the Easter cycle from the old computation on a basis of 84 years to the new cycle of 532 of Victorius Aquitaine in 457, the Gallican Church, unlike the Celts, did the same; but when, in 525, the Roman Church adopted the 19 years cycle of Dionysius Exiguus, the Gallican Church continued to use the cycle of Victorius, until the end of the eighth or beginning of the ninth century.

当罗马教会改变了复活节周期从旧的计算的基础上， 84岁的新一轮的532 Victorius阿基坦在457的高卢圣教会，不同的是凯尔特人，也做了同样的；但是当在525 ，罗马教会通过19年周期的狄奥尼修斯艾克西古斯的高卢圣教会继续使用周期Victorius ，直至第八或年初的9世纪。

Based on achievements in technique innovation of nuclear power fuel cycle engineering of pressurized water reactor since the late 1970 s,the progress is sumed up and classified as three trains of innovation thoughts:oncethrough cycle reprocessing fuel cycle and reprocessing-partitioning-transmutation cycle.

基于近30年来压水堆核电燃料循环工程技术创新取得的成果，将其发展进程归纳成三种有代表性的创新思路：一次通过的开式循环、后处理燃料循环和后处理-分离-嬗变闭路循环。

The foreign professional athletes of "Three rackets" sport always use the short-cycle of the annual cycle model; Chinese athletes still use the annual two-big cycle and multi-big cycle model in table tennis and badminton.

三拍项目国外职业运动员多采用中小周期的年度周期模式，乒乓球、羽毛球项目中国运动员仍然采用年度双大周期及多大周期的模式。

In addition, you can use a number of combined cycle, such as mercury - a class 2 water steam cycle, steam - gas-fired combined cycle and so on to get closer to Carnot cycle in order to achieve higher thermal efficiency.

此外，还可以采用一些联合循环，如汞—水之类的两汽循环、蒸汽—燃气联合循环等来接近卡诺循环以达到更高的热效率。

Bucher diagram is that the carnot cycle characteristics, a simple and practical m-xt Bucher diagram paper, the start of the carnot cycle is used to express the prototype, after the further improvement and to promote its application in various thermodynamic cycle as Rudolf circulation, three heat cycle, reverse circulation within a brief reversible, makes people aware of the limits using Bucher diagram, the rich contents.

Bucher图是表示卡诺循环特性的一种简单而又实用的新图，本文对Bucher图的最开始的用于表示卡诺循环的原型，之后对其进一步的改进和推广使其应用于各种热力学循环如狄塞尔循环，三热源循环，逆向内可逆循环等作了简要的叙述，使得人们了解到Bucher图的使用范围的广泛，内容的丰富。

Using data of the traffic flows in the current cyc le and the cycle before, the traffic flows in the next cycle may be estimated th rough the linear pre-estimation method and each phase time in next cycle is dete rmined with GA optimizing method.

通过对本周期及前一个周期的车流量进行实时测量，采用线性预估方法，对下一个周期的车流量进行预估，以最大通行能力为路口模型控制性能指标，从而确定下一周期的相位配时方案。

Firstly, this paper investigated roundly the basic theory of the ambient vibration method, analyzed systematically the main methods of ambient vibration identification, and discussed their advantages and disadvantages. Then we established the finite element model of structural dynamics and test model and analyzed their mutual relation, improved MUSIC method and correlation function method in the harmonic identification, and gave the subspace method and correlation function method based on continuous model, which is multi-harmonic retrieve technique. And then, this paper also puts forward least-squares method to estimate directly structural physical and modal parameters; this approach replaced the derivative operator in the continuous time model with an equivalent approximation, and estimated the physical parameters of structures by the least-squares method. And then, we discussed the approximation methods of derivative and their convergence. Finally, this paper researched the modal parameters of a frame structure by the simulation and test. The results demonstrated that subspace method, correlation function method and the least-squares method are effective and stable respectively.

全面地研究环境振动识别方法的基本理论，系统地分析环境振动识别方法的主要方法，讨论它们的优点和不足；建立结(来源：6666AB44C论文网www.abclunwen.com)构动力学的有限元模型和试验模型，分析有限元模型与试验模型之间的相互关系；改进谐波识别中的MUSIC识别方法和相关函数识别方法，给出大型结构模态识别的连续型的子空间分解识别方法和相关函数识别方法，这个方法是多元谐波识别方法；提出直接识别结构的物理参数和模态参数的最小二乘方法，这个方法利用导数的近似值代替导数值，并运用最小二乘方法识别结构的物理参数，讨论导数近似值的选择方法，分析算法的收敛性；通过仿真计算和模型实验，研究高层框架结构的模态参数识别，验证子空间分解识别方法、相关函数识别方法和最小二乘识别方法的有效性，算法的稳定性；从理论和试验两个方面分析和研究噪声对识别精度的影响，影响识别精度的因素，以及部分模态成分的模态参数不能够被识别的原因。

The revised Hargraves formula has the best result compared with Penman-Monteith formula, the average relative error is 6%, moreover, Hargaves method requiring the least data in calculating ETo, so this method has the high actual application value; The following are Radiation method and Blaney-Criddle method ; The worst is the Pan method, even after revised, the highest and average relative error is 36% and 10%, respectively. So, in this study, Penman-Monteith method, Radiation method, Blaney-Criddle method and Hargraves method are used except Pan method.

在这四种方法中，修正后的Hargraves方法与彭曼—蒙特斯方法的计算结果符合最好，其平均相对误差为6％，而Hargraves方法需要的气象数据只有计算时段的最高、最低温度，该方法具有较高的实际应用价值；辐射方法和布兰尼—克里德尔方法次之；蒸发皿方法最差，即使在修正后，E601型蒸发皿的最大误差也高达36％，平均为10％，所以在预报系统的研究设计中采用了除蒸发皿方法之外的四种方法。

In contrast to the ubiquitous rising-sun-with-rays military flag of the Japanese, Chinese banners and ensigns feature a range of designs.

与遍地都是的太阳军旗不同，中国人的旗帜和徽章设计得各式各样。

From their small corner of Feng's Guangzhou headquarters -- a jumble of pink leashes, squeezable rubber steaks, and plastic doggy Santas for Fido's stocking -- Soleil's designers come up with at least five new products a month.

从Feng 设在广州总部的产品展示柜台上可以看到，Soleil的设计师每月至少设计出5件新产品。