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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

During the course of the short-cycle correlation, This paper assembleds the descending cycle of early cycle and the ascending cycle of late cycle, which are conjointed together , begin to correlate and draw the ichnography . By this method , writer can consider the two connected sandbodies as one unit to analyse , and can contact the sand units of exploitatition in the oilfield. Then analyse the distributing and the continuity of the sandbodies in the medium-cycle, the distributing and the continuity of oil and gas in the short-cycle.

每个中期旋回包括 3-4 个短期旋回,在短期旋回的对比中,将垂向上相邻的早期短期旋回的下降半旋回和晚期短期旋回的上升半旋回组合到一起进行对比划分和平面成图,这使得相邻两套的粉砂岩作为一个单元进行分析,与油田开发中的小层紧密的结合到一起。

Main points of the thesis are as follows:(1) The main function models and information relations between these models in the CAPP system in the integrated CAD/CAM project are explained, a new process plan design method is mentioned based on analyses of process plan design. This method is based on the process plan prototype, and develops the prototype little by little until the perfect process plan is completed, the prospect of this method is discussed;(2) The feature abstracting rules and methods are studied, a feature coding system is developed based on GT, all the defined feature models are described by the mathematical methods;(3) The integrated method of CAD/CAPP based on features is mentioned, this method defines a feature exchanging model, and develops an interface software to exchange design data into technology data base, this technology data base includes the design and technology information of the parts;(4) The process plan reasoning method based on features is created, this method uses Mycin algorithm to solve the reasoning of the feature process plan. A process plan making system is developed. In this system part process plan is made by the semi-intelligent method, this method uses the feature reasoning, feature process chain searching and man-computer talking together;(5) The main structure of the HOPE system is introduced, a shaft drawing and its process plan made by this system are shown, the data process problem in process drawing making is also discussed;(6) A new method of describing process rule is introduced, this method uses IfThen rules and neural nets weight values together to describe process rules, the BP algorithm is adopted in this method;(7) The optimal machining sequence problem of the process steps on the same fixturing is discussed, optimal algorithms SA is applied to solve this problem, the result shows that this method is more efficient than the traditional method;(8) All jobs in the thesis are summarized. In order to carry out the research in the future, some rational proposals are given.

全文论述的内容主要有下列几个方面:(1)较为系统地阐述了CAD/CAM一体化工程项目中CAPP系统的主要功能模块及其信息流程,在分析工艺设计过程的基础上,提出了基于特征原型的渐进式工艺设计方法,并探讨了该方法的应用前景;(2)研究了零件特征提取的原则和方法,在成组技术的基础上,开发了一套基于特征的分类编码系统,并在提取零件特征的基础上,定义了零件特征的数据模型,开发了基于轴类零件特征的参数化设计系统;(3)研究了基于特征的CAD/CAPP集成的方法,通过定义零件特征数据模型的存储结构,开发了将设计数据转换到工艺数据库中的接口程序,为后续的CAPP系统提供必要的零件形状和工艺信息;(4)研究了基于特征的工艺推理方法,将Mycin算法应用到工艺决策中,开发了基于特征的工艺推理及工艺方案生成系统,在系统中综合运用特征推理、特征加工工艺链查询及人机对话相结合的半智能化方法确定零件加工工艺;(5)介绍了整个HOPE系统的总体结构,通过运行HOPE系统绘出了一轴类零件的零件图,并生成了该零件的加工工艺方案,初步探讨了基于特征的工序图生成技术中数据处理问题;(6)研究了工艺设计过程中知识如何有效表示的问题,提出了将显式的IF-THEN规则表示与隐式神经网络权值表示相结合的工艺知识表示方法,并利用BP算法进行了工艺知识表示的初步研究;(7)研究了工艺设计过程中同一安装下工步优化排序问题,利用神经网络中基于模拟退火的理论来解决该工步优化排序问题,实践证明该方法较传统的算法具有更高的效率;(8)总结了本文所进行的研究工作,为今后进一步开展工作提出了较为合理的建议。

Then the combined cycle with the same gas turbine systemfuelled by coke oven gas was simulated by this method, and steam parameters ofdifferent steam cycle modes were optimized by thermodynamic method, pursuingthe target function of the maximization of combined cycle efficiency;based on foreoptimized results, using the thermoeconomic method of matrix pattern, the mode ofsteam system and the pinch point temperature difference under the optimal modewere optimized, pursuing the target function of the minimization of generating cost,and thereby the optimal mode of combined cycle fuelled coke oven gas wasobtained.

将此方法应用于燃气轮机系统设备不变时的燃用焦炉煤气的情况,并以联合循环效率最大为目标函数,对不同蒸汽循环方式时的蒸汽参数进行了热力学优化;在此基础上,利用矩阵模式热经济学法,以联合循环最小发电成本为目标函数,对蒸汽循环方式及最佳蒸汽循环方式下的节点温差进行了优化,从而初步得到了燃用焦炉煤气联合循环的最佳形式。

In the economic cycle, it is necessary to pay attention to the inventory cycle, corporate capital spending cycle and the real estate cycle, the three-cycle changes.

在经济周期中,要注意库存周期,企业资本支出的周期和房地产周期,三个周期的变化。

ABSTRACT Global carbon cycle is a very important issue in the study of global climate change. Forest ecosystem carbon cycle is one of the important part of global carbon cycle, but at the present time there are considerable uncertainties in the research of global carbon cycle, which also exist in forest ecosystems carbon cycle research, especially in the estimation of carbon storage of region forest ecosystems and CO〓 fluxes exchanging with other systems.

全球碳循环是全球气候变化研究的核心问题之一,森林生态系统碳循环是全球碳循环的重要组成部分,目前在全球碳循环的研究中还存在很大的不确定性,这种不确定性同样存在于森林生态系统碳循环的研究中,尤其表现在对区域森林生态系统碳库大小及其与大气CO〓交换通量的估计上。

Analytical results showed that the thermal efficiency and exergy efficiency increased with increasing condensation temperature of Rankine cycle, inlet pressure of turbines of Rankine cycle and LNG cycle, and decreased with increasing outlet pressure of turbines of Rankine cycle and LNG cycle.

分析结果表明,混合动力循环热效率和可用能效率随朗肯循环冷凝温度升高、朗肯循环透平进口压力和液化天然气循环透平进口压力增大而提高,随朗肯循环透平出口压力和液化天然气循环透平出口压力增大而降低。

The energy balance equations and exergy balance equations of heat exchanger and power equipment in the combined power cycle were established.Taking condensation temperature of Rankine cycle, inlet and outlet pressure of turbine of Rankine cycle, inlet and outlet pressures of turbine of LNG cycle as key parameters, the influences of these parameters on the thermal efficiency and exergy efficiency of the combined power cycle were analyzed.

建立了混合动力循环中换热和动力设备的能量平衡方程和可用能平衡方程,并以朗肯循环冷凝温度、朗肯循环透平进出口压力、液化天然气循环透平进出口压力为关键参数,分析了上述关键参数对混合动力循环热效率和可用能效率的影响。

The energy balance equations and exergy balance equations of beat exchanger and power equipment in the combined power cycle were established. Taking condensation temperature of Rankine cycle, inlet and outlet pressure of turbine of Rankine cycle, inlet and outlet pressures of turbine of LNG cycle as key parameters, the influences of these parameters on the thermal efficiency and exergy efficiency of the combined power cycle were analyzed.

建立了混合动力循环中换热和动力设备的能量平衡方程和可用能平衡方程,并以朗肯循环冷凝温度、朗肯循环透平进出口压力、液化天然气循环透平进出口压力为关键参数,分析了上述关键参数对混合动力循环热效率和可用能效率的影响。

The work had been carried out can be summarized as follows: 1. Thermal cycle analysisBased on the classical thermodynamics, influence of isobaric reheat and isovolumetric reheat on cycle output is discussed. Based on the finite time thermodynamic theory, the thesis discusses the optimization of Rankine cycle, Brayton cycle and Stirling cycle between constant temperature heat source and constant temperature heat sink or between variable temperature heat source and constant temperature.

第一、循环优化分析:采用经典热力学研究了等压再热与等容再热对循环功的影响;采用有限时间热力学深入研究了恒温热源一恒温冷源和变温热源一恒温冷源液氮发动机朗肯循环、布雷顿循环和斯特林循环的优化和对比;着重研究了工质导热率与热机换热器总导热率之比对循环性能的影响、高温侧换热器导热率与低温侧换热器导热率之比对循环功率的影响等内容;得到了一些对循环和系统设计具有现实指导意义的结论。

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相关中文对照歌词
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您的推杆方式,通过36个有趣的填孔迷你的三维设计的课程,以提升绿党,掩体,桥梁和水的危害,除其他疯狂的障碍。

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