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Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Stephen, obviously addressed, looked down on the photo showing a large sized lady with her fleshy charms on evidence in an open fashion as she was in the full bloom of womanhood in evening dress cut ostentatiously low for the occasion to give a liberal display of bosom, with more than vision of breasts, her full lips parted and some perfect teeth, standing near, ostensibly with gravity, a piano on the rest of which was In Old Madrid , a ballad, pretty in its way, which was then all the vogue.

248经对方这么明确地一说，斯蒂芬就低头端详起照片来。那是个高大丰腴的女人，风华正茂，充分散发出肉体的魅力。她身着夜礼服，炫耀般地将脖领儿开得低低的，尽量突出那对轮廓鲜明的乳房。饱满的嘴唇是张着的，露出几颗皎齿，显得蛮庄重地伫立在钢琴旁边。乐谱架上摆着挺好听的民歌《在古老的马德里》的乐谱，当时正流行的。

The CAG repeat lengths in the AR gene were analyzed by somatic microsatellites. Results A significant difference was found in the CAG repeat number between the male acne patients （22.70 ± 3.09） and male controls （23.48 ± 2.83, P = 0.046）, but not between the female cases and controls （23.41 ± 2.87 versus 23.85 ± 0.21, P = 0.12）. In order to assess the risk associated with CAG repeats, the male and female subjects were dichotomized based on the median repeat length in the corresponding control group as the arbitrary cut-off point.

结果 男性病例组和对照组的CAG重复均数分别为22.70 ± 3.09和23.48 ± 2.83，两组之间差异有统计学意义（P = 0.046）；将对照组中CAG重复次数的中位数23作为分割点分组比较，长/短CAG片段在男性病例和对照中的分布差异有统计学意义，携带有CAG短片段的男性较携带CAG长片段的男性患痤疮的风险性明显增加（OR值2.07；95%可信限为1.21 ～ 3.54）。

I observ'd, that the two who swam, were yet more than twice as long swimming over the Creek, as the Fellow was, that fled from them: It came now very warmly upon my Thoughts, and indeed irresistibly, that now was my Time to get me a Servant, and perhaps a Companion, or Assistant; and that I was call'd plainly by Providence to save this poor Creature's Life; I immediately run down the Ladders with all possible Expedition, fetches my two Guns, for they were both but at the Foot of the Ladders, as I observ'd above; and getting up again, with the same haste, to the Top of the Hill, I cross'd toward the Sea; and having a very short Cut, and all down Hill, clapp'd my self in the way, between the Pursuers, and the Pursu'd; hallowing aloud to him that fled, who looking back, was at first perhaps as much frighted at me, as at them; but I beckon'd with my Hand to him, to come back; and in the mean time, I slowly advanc'd towards the two that follow'd; then rushing at once upon the foremost, I knock'd him down with the Stock of my Piece I was loath to fire, because 1 would not have the rest hear; though at that distance, it would not have been easily heard, and being out of Sight of the Smoke too, they wou'd not have easily known what to make of it: Having knock'd this Fellow down, the other who pursu'd with him stopp'd, as if he had been frighted; and I advanc'd a-pace towards him; but as I came nearer, I perceiv'd presently, he had a Bow and Arrow, and was fitting it to shoot at me; so I was then necessitated to shoot at him first, which I did, and kill'd him at the first Shoot; the poor Savage who fled, but had stopp'd; though he saw both his Enemies fallen, and kill'd, as he thought; yet was so frighted with the Fire, and Noise of my Piece, that he stood Stock still, and neither came forward or went backward, tho' he seem'd rather enclin'd to fly still, than to come on; I hollow'd again to him, and made Signs to come forward, which he easily understood, and came a little way, then stopp'd again, and then a little further, and stopp'd again, and I cou'd then perceive that he stood trembling, as if he had been taken Prisoner, and had just been to be kill'd, as his two Enemies were; I beckon'd him again to come to me, and gave him all the Signs of Encouragement that I could think of, and he came nearer and nearer, kneeling down every Ten or Twelve steps in token of acknowledgement for my saving his Life: I smil'd at him, and look'd pleasantly, and beckon'd to him to come still nearer; at length he came close to me, and then he kneel'd down again, kiss'd the Ground, and laid his Head upon the Ground, and taking me by the Foot, set my Foot upon his Head; this it seems was in token of swearing to be my Slave for ever; I took him up, and made much of him, and encourag'd him all I could.

在他们和我的城堡之间，有一条小河。

I can feel his standpoints and courage to the contemporary culture. But he is unable to use pungent language to criticize the tradition. He uses persuasive dreamland and seemingly sober state to face to it. In another aspect he has cut his ink language off the relations to traditional landscape paintings. Let the audiences in front of his works realize and moved, let the mark shape, kinking, variation objects appear repeatedly in his pictures, so that he subverts all requests in art to traditional pictures. Based on the above, I dare to conclude Liuming is not a "Chinese landscape painting painter", although on the "www. yanzhoucn. com" he insists on calling oneself "the renowned Chinese painting painter".

我能感受到刘鸣对当代文化所持有的立场和勇气，但他又无法用一种辛辣的语言去批判传统，他婉转地用梦境、用似醒非醒的状态去面对；而在另外一个层面，他揭然斩断了传统山水画与其艺术的关系，让观众在其作品前省悟和木讷，让符号状、扭结、变异的物体，在其画面上反复出现，彻底地颠覆了传统画论中对山水画的所有要求；基于上述，我敢断定刘鸣绝不是一个中国山水画画家，尽管他在 yanzhoucn.com 网页上坚持称自己为&著名国画家&。

Space concept--sandwich biscuit or hamburger In respect of the present system of Crystal Island, space is singularized and homogenized in function setup; behavior activity assumes single space-time mobility, CBD in the daytime and empty city in the evening; the ground space was cut by fast roads, the space interface and walking system are not continuous; underground space is short of centralized management and the transfer system is very low in efficiency.

空间 水晶岛空间现有体系，功能设置单一化、均质化；行为活动单一时空流动性，白天CBD、晚上空城；地面空间被快速道路切割，空间界面与步行体系不连续；地下空间各自为政，换乘体系效率大为低下。

In the whole growth period of spring wheat, the response was different in yield and phase growing on climate change, such as the appearing time of sprouting, jointing, tasselling and maturity being ahead but milking being postponed. That is to say, it shortened the spring wheat's nutrition growth stage of seedingsprouting, elongated the sproutingjointing stage, cut down the generative growth in the jointingtasselling phase, prolonged the tassellingmilking stage, and shortened the milking~maturity stage. These finally resulted in the shortening of whole growth period.

在春小麦整个生长发育过程中，其阶段生长和产量对气候变化的响应不完全一致：气候变化使该地区春小麦出苗期、拔节期、抽穗期和成熟期的出现时间均提前，而乳熟期出现时间推迟，导致播种—出苗期的营养生长阶段缩短、出苗—拔节期的营养生长阶段延长、拔节—抽穗期的生殖生长阶段缩短，抽穗—乳熟期的生殖生长阶段延长，乳熟—成熟期的生殖生长阶段缩短，最终造成全生长期缩短。

In the whole growth period of spring wheat, the response was different in yield and phase growing on climate change, such as the appearing time of sprouting, jointing, tasselling and maturity being ahead but milking being postponed. That is to say, it shortened the spring wheat's nutrition growth stage of seeding-sprouting, elongated the sprouting-jointing stage, cut down the generative growth in the jointing-tasselling phase, prolonged the tasselling-milking stage, and shortened the milking-maturity stage. These finally resulted in the shortening of whole growth period.

在春小麦整个生长发育过程中，其阶段生长和产量对气候变化的响应不完全一致：气候变化使该地区春小麦出苗期、拔节期、抽穗期和成熟期的出现时间均提前，而乳熟期出现时间推迟，导致播种－出苗期的营养生长阶段缩短、出苗－拔节期的营养生长阶段延长、拔节－抽穗期的生殖生长阶段缩短，抽穗－乳熟期的生殖生长阶段延长，乳熟－成熟期的生殖生长阶段缩短，最终造成全生长期缩短。

Implementation of the aforementioned circle shear machine would result in major complications in the handling of the metal sheet, since now the metal sheet must be rotated in the middle of the automated line in order to generate a hole cut-out by the circle shear machine.

实施上述圈剪切机，将导致重大并发症，在处理的金属板，因为现在的金属板，必须在旋转中的自动线，以产生一个洞，割出所圈剪切机。

The entrance section of FuXi Double-arched Tunnel is modeled in the fact of factual shallow buried and unsymmetrical pressure condition. Adopting the three heading construction method to simulate twelve kinds of construction projects, confirm reasonable construction orders and left and right tunnel face interval, systematically analyze and research the changing law of the tunnel deformation and surrounding rock stress in soft and cracked condition of surrounding rock,bring forward pertinent advices against construction disease; Build the deep buried tunnel model in middle section of tunnel , separately adopt the central heading bench cut benching method and the central heading full cross method to simulate ten kinds of construction projects, establish the reasonable construction orders, sidestep length and tunnel face interval, which are due to the deep buried tunnel, systematically analyze and research the change law of the tunnel deformation and surrounding rock stress in the section with good surrounding rock condition, bring forward the reasonable construction projects which can make sure of construction safety and save construction time; Because the central wall is disturbed many times during constructing process, its displacement and stress distribution is very complex.

富溪隧道进口段依照实际的浅埋偏压状况建立模型，采用三导洞法开挖模拟了12种方案，确定了合理的施工顺序和左右主洞掌子面错距，系统地分析研究了软弱破碎围岩浅埋偏压段施工的隧道变形和围岩应力变化规律，提出了防止施工病害的针对性建议；洞身段按深埋隧道建立模型，分别采用中导洞台阶法和中导洞全断面法模拟了10种方案，确立了适合深埋隧道的合理施工顺序、台阶长度和掌子面错距，系统分析研究了围岩较好深埋段施工的隧道变形和围岩应力变化规律，提出了兼顾施工安全和节省工期的合理施工方案；由于受到多次扰动，中隔墙的位移和应力变化规律较为复杂，通过对各种方案的对比分析，得出了中隔墙在施工不同阶段的位移变化规律、应力变化规律和应力分布规律，尤其对于浅埋偏压段隧道施工时中隔墙位移和应力变化规律做了深入研究，提出了合理的施工建议。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。