查询词典 cut both ways

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Child scissors taipans both under the realistic graphics and image, they have to use skilled civil paper-cut technique, the tentacles of animals, the characters hair, plant growth dynamics, the more subtle cut child care more vividly.

大班幼儿剪刀下的图形既逼真又形象，他们已经能熟练地运用民间剪纸技法，动物的触角，人物的发丝，植物的生长动态，越是细微之处幼儿越是剪得惟妙惟肖。

For afore the harvest, when the bud is perfect, and the sour grape is ***pening in the flower, he shall both cut off the sp***gs with pruning hooks, and take away and cut down the branches.

18：5 收割之先，花开已谢，花也成了将熟的葡萄，他必用镰刀削去嫩枝，又砍掉蔓延的枝条。

Cut file, sawing skills by learning the grip, standing position, body movements, and many other points force factors and the impact of the traditional grip of the emergence of file-grip handle to stand up after slightly outside the site of the palm, when a file cut Force, after the file handle a gradual shift,"hold" to "grasp", resulting in the introduction file enough; saw grip handle full point higher than the thrust of the saw blade to install center hole of about 30mm, the high point of thrust is likely to result in the bow saw On both sides of the swing, resulting in a broken saw blade and saw seam askew.

锉削、锯削技能的学习是受握法、站立部位、身体动作、作用力点等诸多因素的制约和影响的，传统的握法出现握锉柄后顶住部位稍偏离手心，锉削时一用力，锉柄逐渐后移，&握&变成&抓&，造成锉刀推出力度不足；满握锯柄的推力点高于锯条安装孔中心约30mm，偏高的推力点很容易造成锯弓向两侧摆动，造成锯条折断及锯缝歪斜。

Do fill gaps in the "operation" could be first ribbon in the "loophole" in the region cut off, and then cut the ribbons,"weld" together,"welding" as long as the first band at the interface at both ends of alignment and It is a little overlap, and then use transparent tape to the inside of the two ribbons together interface adhesion; if afraid of sticky tape is not strong, you can use thin wire in the ribbon at the joint and under the edge of the seam on the edge of a needle (This does not affect the normal work of the ribbon, ribbon work, after all, the main use of the middle region).

在做补漏&手术&时，可以先将色带中有&漏洞&的区域剪掉，然后再将剪断的色带&焊接&起来，&焊接&时只要先把色带两端的接口对齐并稍微有一点重合，然后使用透明胶带将色带内侧的两个接口粘连在一起；要是怕胶带粘性不强的话，可以用细线在色带接头处的上边缘和下边缘各缝上一针(这样不影响色带正常工作的，毕竟色带工作时主要使用中间的区域)。

The reason for regarding the architectural education of France and America in this period as an organic whole of Academicism system is that both of them treated the idea of"Beaux-Arts"as key, and there were common systematic characteristics between them in thoughts and methods: universities and professional bodies ran schools cooperatively all the time; the methodology is based on the combination of university teaching with atlier teaching; they both belived in"antique"and accepted Ecleticism; they both had obvious aestheticism and strict attitude in study; they both had the intention to adapt to the practical situation in their respective countries. The whole dissertation contains about 185, 000 words, 132 illustrations and 12 forms

之所以将法、美两国这一时期的建筑教育视为紧密相关的"学院派"体系，是因为它们都以"鲍扎"思想为核心，在认识论与方法论上有着体系化的共性特征：学校与行业学会始终协同办学；教学模式以大学体系和画室制结合；学术上有尚古和折衷主义倾向；治学上有着明显的唯美和严谨的风范；实际运作中又都有着为适合各自国情发展的意识。

Power cable route protection zone: the areas between two parallel lines formed by 0.75 meters on both sides of the ground mark of underground cable route; for sea-bottom cables, the zones should be the water surface between the two parallel lines formed by two nautical miles on both sides of the line (100 on both sides of the line in port) and no less than 100 meters on both sides of the line in rivers (not less than 50 meters for mid-sized and small rivers).

电力电缆线路保护区：地下电缆为线路两侧各零点七五米所形成的两平行线内的区域；海底电缆一般为线路两侧各二海里，江河电缆一般不小干线路两侧各一百米（中、小河流一般不小于各五十米）所形成的两平行线内的水域。

The second House and his Lord signifies the gain of the Querent : and the eighth House and his Lord signifies the gain of his fellow, or his part : and if they be both good, they shall both gain ; and if both evil, they shall both lose : and if one be good and the other evil, he whose significator is good, shall gain ; and he whose is evil, shall lose.

2宫及其守护星象征的问卜者的收益；8宫及其守护星象征着对方或其隶属之方的收益；如果两方都情况良好，则双方皆得利；若都不佳，则双方皆损；若一方吉而另一方凶，则吉方获益，凶方受损。

The comparative research results showed that:(1)Thediurnal variation of the net photosynthetic rates in the aquatic leaves was a "double-peak", with an various "midday-decline", the time of the peak value appeared at about 10:00 A.M. and 13:00 P.M., while that in the xeric leaves was showed with invarious peak value and "midday-decline"; The diurnalvariation of transpiration ratesof both aquatic and xeric leaves appeared as a single-peak, the appearance time of peak value of the latter was earlier than the former and gradually decreased in the afternoon; The stomatal conductances appearance time of both of them were close to that of photosynthesisrate ,reached to the maximum in the morning and then gradually decreased: Diurnal variation of intercellular CO2 concentrationshows that: both aquatic and xeric leaves increased at first, and then exhibit a tendency of ascending within narrow range.(2) The net photosynthetic rates, photosyntheticallyactive radiation, stomatal conductances and transpiration ratesof both two kinds of leaves showed positive correlation, The correlation degree among them was prominent, there was significant negative correlation between The net photosynthetic rates and intercellular CO2 concentration.

研究结果表明：(1)水生叶片净光合速率的日变化曲线呈双峰型，出现明显的&午休&现象，峰值出现在10：00左右和13：00左右，但旱生叶片表现出的峰型和&午休&现象不明显；两种叶片的蒸腾速率日变化曲线表现为明显的单峰型，旱生叶峰值出现时间早于水生叶，中午过后逐渐下降；两种叶片气孔导度峰值出现时间和光合速率相近，均表现为上午升高至最高，随后逐渐下降的趋势；两种叶片胞间二氧化碳浓度日变化表现为：整体上先下降，然后有一上升趋势：(2)两种叶片的光合速率与光合有效辐射、气孔导度、蒸腾速率呈正相关，全部达到显著水平；光合速率与胞间CO2浓度显著负相关。

You can buy sweetened beverages, if that suits you.

你若喜欢的话，可以买些含有糖精的混合饮料。

As many of you are probably aware, Nick and Vanessa were recently faced with an embarrasing sex scandal after naked pictures of the two getting jiggy with it in a hot tub surfaced the net.

正如很多你可能知道，尼克和 Vanessa 最近面临一个尴尬的绯闻事件后，赤裸裸的图片两个越来越jiggy与它在浴缸中的一个热点浮现净。