查询词典 culturing

Researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects;2 Combining with the techniques of cell engineering (nuclear transplantation and electric fusion etc.), and through the process of culturing histiocyte of fish, cryopreservation and re-culture after thawing, carrying out somatic cell breeding of fish.

多年来，国内外学者对各种鱼类精液的冷冻保存进行了大量的系统研究，目前这项技术已达到实用水平，并日益发挥作用；二是对鱼类培养的组织细胞冷冻保存，通过鱼类细胞的培养、超低温冻存、解冻后再培养过程，结合细胞工程技术进行体细胞育种；大量的研究结果表明鱼类体细胞核具有发育的全能性，随着细胞培养技术、细胞工程技术日益发展成熟，完全具备实现鱼类物种种质长期保存的理论基础和技术条件。

Recently ,because of the wastewater discharge from living , net culturing, hoggery, runoff, etc., especially, excessive loading of dissolved and particulate organic matter and inorganic nutrients, the quality of reservoir has deteriorated.

近年来，由于生活废水、生产废水、网箱养鱼、养猪、地表径流等，尤其是大量的溶解态的有机和无机营养物质进入到水库当中，造成水质恶化。

According to the basicqualities, the culturing aims and the special action and position of the morality curriculum inmiddle schools ,the writer adopts some learners' opinions and generalizes the six roles ofteachers who teach morality curriculum: a. Knowledge Imparter b. Students' Guide c. Teaching Designer d. Developer and Builder of Curriculum Rosource e. Teaching and Educational Researcher f.

根据初中思想品德课基本性质、培养目标、及其特殊的地位和作用，根据初中生成长需要，结合了一些学者的观点，把新课程中思想品德课教师定位为六大角色：知识的传授者、学生的引导者，教学的设计者、课程资源的开发者和建设者，教育教学的研究者，品德示范者。

Objective:To explore the culturing of human ligamentum flavum cells in vitro.Method:HLF were harvested during surgery for thoracolumbar burst fractures.

目的：探讨成人黄韧带细胞的体外培养方法，为研究黄韧带退变的发病机制奠定基础。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

The result indicated:① In accordance with culturing in the medium containing MS, SH, N6 macroelement, respectively, the effect of MS or SH macroelement in the primary culture is better than that of N6 macroelement, and the effect based on N6 macroelement is the best from 5th to 9th subculture.

结果表明：①在含MS、SH、N6的大量元素的培养基上的初代培养，MS或SH的效果好于N6，愈伤组织第5~9次继代培养中，N6大量元素培养基的继代培养效果最好。

These results suggest that it is feasible to produce artificial muskrat scent using cell engineering by isolating, culturing cystglanitd epithelial cell and furthmore establishing a musdrat cystgland epithelial cell line.

提示，通过体外分离培养麝鼠香囊腺上皮细胞，建立香囊腺干细胞系，进而用细胞工程方法大量生产人工麝鼠香是可能的。

His observations of fungi in their natural habitat and his methods of culturing them enabled him to work out their developemt and life cycles, thus laying down the foundations of modern mycology.

他在真菌的天然栖息地观察真菌，并通过培养真菌的方法来获得有关它们发育和生活史的相关知识，继而建立了现代真菌学的基础。

Because of the slow growth rate, that increases the contaminant opportunity by other micro organisms, for these reason it risen the difficulty of culturing and purifying the nitrobacteria, and limit the counting method.

因自营菌生长速度缓慢，造成遭受其他微生物污染的机率上升，所以更增加了硝化菌在培养及分离上的困难，以及计数上的限制，使得现今环境中硝化菌的实际数量及其计数方式仍存有许多争议。

All of the specimens collected were divided into two parts, one part for immunohistochemical staining after fixated by 4% paraformaldehyde, the other part for culturing fibroblasts.

所有取材部位未经任何治疗，标本经无菌取材后分成2份，其中一份用40 g/L多聚甲醛固定后用于免疫组织化学检测，另一份用于成纤维细胞培养。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷