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By culturing on malt extract agar medium with temperature of 25 degrees Celsius,strains w...

结果从红腐乳中分离的两个菌株经鉴定M-1属丛毛红曲菌，M-3属红曲红曲菌。红腐乳中分离的纯培养为红曲属真菌。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

The leaf blade,leaf stalk and stem of Xanthium sibiricum were served as explants,MS medium added different kinds of hormone and proportions was used for inducing and culturing the callus of Xanthium sibiricum.

以苍耳叶片、叶柄及茎段为外植体，在MS附加不同种类和浓度激素配比的培养基上，对苍耳愈伤组织进行诱导培养，并对生长迅速、性状好的愈伤组织进行了研究。

Can improve the survival ratio of seeding. Culturing in hormone media can make meristem tip survive once time, and the result is better than two-time-cultivation.3、The meristem tip with two phyllomes(0.3-0.4mm in size)were found to suitable for virus-free seeding culture that were cut from aseptic seeding, which led to higher survival and virus-free ratio, up to 72.1%and 54.2%.

在培养基中添加椰汁、香蕉汁等有机添加物可以明显提高成苗率。3、采用二次剥离茎尖的方法，从无菌试管苗上切取带2个叶原基(大小0.3-0.4mm)茎尖分生组织培养，成苗率为72.1%，脱毒率为54.2%。

To get the highest cell concentration and spore forming rate, we optimized the culturing conditions.

为使酪酸梭状芽孢杆菌的菌体浓度和芽孢转化率都达到最大，我们对其培养条件进行了研究。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

Using the technique of culturing shoot apex under aseptic conditions, we found that there is a sensitive stage for the sex expression of earlier flower buds, this stage is located before the second leaf full expansing.

利用黄瓜茎尖离体培养系统结合生物切片技术观察了黄瓜早期发育阶段中成花分生组织的发生及发育情况，结果表明：就黄瓜早期发育的花芽而言，可能存在着一个性别决定的敏感点，而这个敏感点位于第二片真叶展开前的某个阶段，此时的花芽绝大多数尚处在花分生组织阶段。

He began each trial by culturing a thick pea-green soup of algae -- the scum of a pond out of whack -- directly under the bank of noon-lights.

在每一次尝试开始的时候，他都会首先培养出一份浓稠的淡绿色海藻汤——一池紊乱的浮藻——直接置于一排犹如午日阳光的灯下。

He began each trial by culturing a thick pea-green soup of algae -- the scum of a pond out of whack -- directly under the bank of noon-lights.

在每一次尝试开始的时候，他都会先培养出一份浓稠的淡绿色海藻汤——一池紊乱的浮藻——直接置于一排大开的灯下。

Under the condition of water solution and soil culturing, the influence of tetracycline and oxytetracycline on the wheat seed germination, root elongation and shoot elongation were investigated.

在水溶液和土壤培养条件下，测定了四环素和土霉素对小麦种子发芽、根伸长和芽伸长的影响。

She immediately set about undoing the work of the Iconoclast emperors.

她立即著手打倒的工作孤星叛逆者皇帝。

As noted in the classroom management section, establishing the classroom rules with your homeroom or co-teacher is very important.

在上面班级经营部分我们已经提及和你的协同教师一同建立班级常规是重要的。