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Geotrichum candidum was cultured in liquidized WWWSI and saccharificated WWWSI respectively and the varitity of reducing sugar, biomass, the activities of glucoamylase and proteinase,amino-N,pH in the culturing process were studied,The results showed that the Geotrichum candidum grew better and gave high biomass in saccharificated WWWSI .

将白地霉分别培养在液化及液化后再糖化的小麦淀粉废水中，对培养过程中的降糖、生物量、蛋白酶和糖化酶活力、氨态氮、pH值的变化进行了研究。

Preliminary studies on the ecological characters of freshwater cyanophage were carried out under both field condition and culturing system. The field investigation result showed that 6 in 19 water samples contained cyanophages lysing plectonema and the lysis phenomenon were readily observed without concentrating the water.

对淡水噬藻体的生态学进行了初步研究，包括淡水中噬藻体分布及感染力的调查与人工混合培养条件下噬藻体裂解宿主引起微生物和藻类种群变化的研究。

Methods Primary human embryonic germ cells were obtained by tissue culturing of embryonic genital ridges and mesenteries from the embryos aged 8 weeks, and then were passaged on the 8th and 16th day using pick-up and digestive passage method. Three kinds of digestive juice were used.

取8周人胚胎的生殖腺嵴和肠背系膜，进行组织块体外培养人胚胎生殖细胞，选择原代培养第8天和第16天的细胞，采用挑取传代法和消化传代法，其中的消化传代法采用三种不同的消化液进行传代培养，记录各代培养的细胞集落数。

Research on rhizosphere microbe is essential for microbial ecology and industrial biotechnology. However, the inability of culturing most rhizosphere microorganisms (around 99%) in the laboratory obviates the research progress.

根际微生物研究对微生物生态学和工业生物技术开发均十分重要，妨碍其研究进展的主要原因是99%的根际微生物物种在实验室中无法成功培养。

Different callus were obtained by culturing the leave explants of one double flower Petunia plant on the media supplemented with different combinations of auxin and cytokinnins.

方法］以重瓣矮牵牛叶片为外植体，使用不同浓度BA与NAA的组合在黑暗条件下诱导愈伤组织。

Therefore, it is urgent to develop a precision production system for culturing soilless Anthuriums in green houses to solve the confronted problems simultaneously. The objective of this project in this year is to develop related technologies for producing Anthurium cut flowers.

有鉴於此，以精致精准农业生产模式概念为主，研发一套火鹤花精准管理的生产体系，一并解决缩短育苗时间、省却添加介质之劳力与物料，及避免土生性病菌感染危害的问题，实为当前火鹤花产业提升之迫切需求。

Therefore, it is urgent to develop a precision production system for culturing soilless Anthuriums in greenhouse to solve the confronted problems simultaneously. The objective of this project in this year is to continuously develop related technologies for producing Anthurium cut flowers.

有鉴於此，以精致精准农业生产模式概念为主，研发一套火鹤花精准管理的生产体系，一并解决缩短育苗时间、省却添加介质之劳力与物料，及避免土生性病菌感染危害的问题，实为当前火鹤花产业提升之迫切需求。

Development of celluloses that are stable outside the cells of microorganisms enables the culturing of fungi and with yeasts on cellulose hydrolyzates.

开发在微生物细胞外部能稳定存在的纤维素酶使真菌和酵母在纤维素水解产物上培养有了可能。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

Methods The astrocytes were isolated from cerebral cortex of neonatal rats which are born within three days, cultivating by DMEM media added with 15% fetal calf serum and depurating by swinging and passaging, to simulate nulli-nutrition model by substituting medium for PBS buffer solution 3h then applying methylprednisolone and recnutrition immediately, we will go on culturing for three days, verificating astrocytes by immunofluorescent technique; detecting activity of AST; determining GFAP of change by PCR.

采用出生3d内的SD大鼠的新生鼠的大脑制成细胞悬浊液后，应用含15%胎牛血清的DMEM培养液中培养，通过摇床和逐渐传代纯化星形胶质细胞，通过PBS缓冲液代替培养基模拟缺营养模型3h后即刻应用甲基强的松龙(10umol/l)，然后继续培养3d，免疫荧光技术鉴定星形胶质细胞；MTT法检测细胞的增殖活性和凋亡；PCR技术测定细胞GFAP表达的变化。

But this is impossible, as long as it is engaging in a market economy, there are risks in any operation.

但是，这是不可能的，只要是搞市场经济，是有风险的任何行动。

We're on the same wavelength.

我们是同道中人。