查询词典 culturing

Culturing of clinical medical special academic rank graduates has carried out for almost 10 years but only several years in military medical university.

医学专业生的培养在我国已经开展了近10年，而军队医学院校则刚刚起步。

High culturing temperature results in short interval between fertilization and eclosion, whereas more days are required at low temperature. The culturing temperature has a significant influence upon the numbers of P.

在连续60天的观察中，发现同一天的受精卵，在实验室中有两个羽化高峰，间隔呈现似半月周期的现象，两峰的间隔会受全期平均温度的影响，高温两峰的间隔短，低温则较长。

The invention discloses a method for cultivating river crab, comprising the following steps: choosing China crab as parent crab, temporary culturing female and male crab separately, then mating; culturing the crab with ovum at temperature under 5 Deg C for more than 2 months; the crab with ovum producing out ovum and the ovum growing into flea larval, megalops larva, young crab, crab strain and adult crab.

本发明是河蟹的养殖方法，包括以下步骤：选择长江系中华绒螯蟹为亲蟹，将雌雄亲蟹分开进行暂养后，进行交配；对抱卵蟹在水温5℃以下饲养2个月以上；抱卵蟹产出卵，生成蚤状幼体，长成大眼幼体；在经仔蟹，长成幼蟹、蟹种再到成蟹。

Mycelium bourgeoning test of solid culturing with liquid strain showed the optimum culture condition was as follows: 4d strain age, 10% inoculation quantity, stirring inoculation mode. Mycelia would overgrow 250ml culturing bottle within 1 week. Compared with traditional technique, the result showed that solid culturing with liquid strain by this means would reduce culture period greatly.

通过液体菌种固体栽培菌丝萌发试验，确定最佳液体菌种种龄为：摇瓶菌种6d，发酵罐菌种4d，接种量为10％，搅拌式接种，以这种方式进行固体栽培，1周内即可使菌丝于250ml培养瓶种发透，与传统方法比较，大大缩短了栽培周期。

A systematic method has been established for primary cultivating of cortical neurons. Observations indicated that the cortical neurons were affixed to the culture after 4h, and the nerve cells had 1-2 small processes. The Nissl's staining results show that the purity(≥92%) of the cortical neurons rose apparently on days 3 and 6 post-beginning of culturing with Ara-C inhibition. The MTT experiment show that the viability of the neurons was the highest,and they are oval,araneiform and pyramidal and formed an integrated neural network on day 6 post-beginning of culturing.

光学显微镜观察结果表明，培养至第4h绝大多数神经元已贴壁生长，胞体上可见1~2个细小突起；培养至第3d、第6d时，Nissl's 染色结果显示，加Ara-C能显著提高神经元纯度（≥92%）；MTT结合形态学观察结果表明，神经元培养至第6d时活性最强，细胞呈卵圆形、蜘蛛状、锥体状等多种形态，细胞胞体饱满，光晕明显，神经网络形成完整。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

Haversion system was clearly observed and matured bone tissues filled the ceramic pores and interconnected with each other. All the result suggested that, taking advantage of"living organic culturing medium"in vivo, culturing composite that consisted of autogenous periostea and biomaterials should be an effective approach to obtain satisfactory bioactive bone substitutes which have the potential to repair load-bearing bone defects clinically.

上述研究表明，1、双相多孔TCP/HA陶瓷是一种优良的骨组织工程支架材料，进一步优化其骨诱导作用后，有可能直接用于再生和重建负重骨干的缺损；2、在目前情况下，以双相多孔TCP/HA陶瓷为支架，通过体内非骨位置培养骨组织细胞形成活体骨修复器件，有可能用于负重骨干缺损的再生和重建。

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

Those parent abalones of wild or artificial selection with good economic characters must be chosen in order to avoid close breeding.. Correct breeding techniques must be adopted to obtain excellent germ cells and larvae. The survival rate and disease resistance of juvenile abalone can be remarkably increasedonce the further selecting and culturing are carried out. The rejuvenation is realized once a good breeding condition for the young and parent abalones is created, the adverse culturing condition and technical maloperation are reduced or eliminated. The survival rate of the breeding and young abalonesincrease about 28.9%-31.2%, the survival rate of the grown abalones is high up to 85%-90%.

通过挑选经济性状好的台湾九孔鲍和人工选育的九孔鲍亲体进行繁殖，避免近亲繁殖，选优除劣，达到正本清源的目的；采用正确的繁殖技术，获得优良的受精卵和幼虫，通过对幼虫进一步选优和培育，使附板后的幼鲍成活率及抗病力得到明显提高；为九孔鲍的苗种和亲本培育创造良好的养殖生态条件，减少和消除了不利的养殖环境和操作技术，达到品种复壮的目的；阐述了野生鲍、复壮鲍、退化鲍三个不同类群的免疫机能具有显著的差异，复壮后的九孔鲍，其免疫机能基本达到了野生鲍的水平；人工育苗和幼鲍的成活率提高了28.9%-31.2%；成鲍的养殖成活率高达85%-90%以上。

Those parent abalones of wild or artificial selection with good economic characters must be chosen in order to avoid close breeding.. correct breeding techniques must be adopted to obtain excellent germ cells and larvae. the survival rate and disease resistance of juvenile abalone can be remarkably increased once the further selecting and culturing are carried out. the rejuvenation is realized once a good breeding condition for the young and parent abalones is created, the adverse culturing condition and technical maloperation are reduced or eliminated. the survival rate of the breeding and young abalones increase about 28.9%-31.2%, the survival rate of the grown abalones is high up to 85%-90%.

通过挑选经济性状好的台湾九孔鲍和人工选育的九孔鲍亲体进行繁殖，避免近亲繁殖，选优除劣，达到正本清源的目的；采用正确的繁殖技术，获得优良的受精卵和幼虫，通过对幼虫进一步选优和培育，使附板后的幼鲍成活率及抗病力得到明显提高；为九孔鲍的苗种和亲本培育创造良好的养殖生态条件，减少和消除了不利的养殖环境和操作技术，达到品种复壮的目的；阐述了野生鲍、复壮鲍、退化鲍三个不同类群的免疫机能具有显著的差异，复壮后的九孔鲍，其免疫机能基本达到了野生鲍的水平；人工育苗和幼鲍的成活率提高了28.9%-31.2%；成鲍的养殖成活率高达85%-90%以上。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷