查询词典 culture-medium

The best explant in the culture was brown leaf,and the explant sterilized in 0.1% aqueous mercuric chloride for 5min has the best effect;At the initial stage in induction,3% glucosan have the best effect following with 1% glucosan+2%sucrose are optimum in the process of culturing;the optimum culture medium is 1/2MS; the preferable medium of callus is 1/2MS+ KT(0.1mg/L)+6-BA(0.05mg/L)+2.4-D(0.4mg/l);differentiation1/2MS+6-BA(0.3mg/l)+NAA0.2+KT(0.05mg/l);and the preferable medium of rooting is :1/2MS+NAA(0.05mg/L).

安祖花组织培养的最佳外植体为棕色叶片；最佳消毒时间为0.1%的升汞消毒5min；最佳碳源：诱导初期3%的葡萄糖的诱导效果最佳，后期培养以2%蔗糖+1%葡萄糖的培养效果较好；最佳基本培养基为1/2MS。

Result The optimum medium formula for protocorm propagation in tissue culture of C. grandiflorium was MS+0.1 mg/L 6-BA. The suitable medium for protocorm induction and differentiation in tissue culture of C. grandiflorum was WS+1.5 mg/L 6-BA+0.3 mg/L NAA with the bud-inducing rate up to 130%. The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA+1.0 mg/6-BA and the survival rate of in vitro plantlets after transplanting was 78%.

结果］大花蕙兰组织培养原球茎粉殖以MS+0.1 mg/L NAA+2.0 mg/L6-BA培养基配方最好；适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L6-BA+0.3 mg/L NAA，诱导出芽率可达130%；适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L6-BA，试管苗移栽后成活率为78%。

Seed culture conditions of Bacillus licheniformis TS-01 were firstly investigated. The results showed that the optimal growth temperature was 40C;the suitable seed culture medium was beef extract soya peptone medium; the optimal initial pH was 7.5; the optimal seed culture time was

首先对地衣芽孢杆菌TS-01的种子培养条件进行了研究，得到的结果为：最适生长温度为40℃；较适合的种子培养基为牛肉膏大豆蛋白胨培养基；最适初始pH值为7.5；种龄为13h～14h。

The steps for preparing insecticide include: first shaking flask for fermentation, adding amidon, soya bean cake powder, cotton cake powder, protease leather, yeast powder, maize milk, magnesium sulfate heptahydrate, monobasic potassium phosphate, dibasic potassium phosphate, calcium carbonate alpha-amylase and make-up water by ratio in culture medium with 100 unit volumes; secondarily filling 100ml culture mediums in triangular bottle, then seeding BTH-13 after disinfection and culturing them until the brood-cell pulls off under finite temperature; third adopting the selected culture medium and fermenting them in full-automatic fermentation tank, acidifying the fermentation liquor after the fermentation, adding the emulsifier, and directly performing spray-drying after the compression of the fermentation liquor to prepare the wettable powder.

制备杀虫剂的步骤是：首先是摇瓶发酵，在100单位体积的培养基中，按比例加入淀粉、豆饼粉、棉饼子粉、蛋白胨、酵母粉、玉米浆、七水硫酸镁、磷酸二氢钾、磷酸氢二钾、碳酸钙、及α-淀粉酶，补充水；其次是在三角瓶中装100ml培养基，灭菌后接种BTH-13，在一定温度培养至芽孢脱落；第三是采用筛选出来的培养基，在全自动发酵罐中进行发酵，发酵结束后发酵液酸化，加入乳化剂，酵液浓缩后直接喷雾干燥制备可湿性粉剂。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

Phosphorus for corn under drought stress the impact of physiological characteristics - 05 graduates graduation session Abstract: In this experiment through the water culture method to cultivate maize seedlings will be planted corn seeds in the absence of P elements in the culture medium of Deficiency, the reprovision of a phosphorus-containing culture medium only for watering.

磷对于干旱逆境下玉米生理特征的影响——05届本科毕业生毕业论文摘要：本实验通过水培法来培育玉米幼苗，将玉米种子栽种在缺少磷元素的缺素培养液中，另配一份只含磷的培养液用于浇灌。

Among different organ explants from ginger lily, only sheaths and leaves cultured in VW medium containing 5mg/l 2, 4-D、0.5mg/l BA、3% sucrose and 200ml/l coconut water for 3 months could be induced to form embryogenic callus. It could produce lots of germinated or ungerminated somatic embryos per culture directly from the surface of calli after culture in MS medium containing 4mg/l BA and 0.05mg/l NAA. The shoots could develop into intact plants with vigorous appearance after 2 months in culture.

叶鞘及叶培植体在含5 mg/l 2，4-D，0.5 mg/1 BA、3%蔗糖及200 ml/1椰子水的VW固体培养基中，经3个月，可诱导产生具体胚分化能力之愈合组织；并於含4 ms/l BA及0.05 ms/l NAA之MS培养基中，直接由此愈合组织的表面产生数个至数十个数目不等之体胚，2个月后可形成一完整植株。

Rabbit zygotes were cultured in sequence culture system that contains: R1 culture medium which is with low concentration of glucose (1.0 mmol/L) and then R2 culture medium which is with high concentration of glucose (3.5 mmol/L).

试验采用含低浓度葡萄糖(1.0mmol/L)的R1培养液和含高浓度葡萄糖(3.5mmol/L)的R2培养液对兔原核期受精卵进行了体外序贯培养，并与传统的RID单一培养进行比较，研究了兔早期胚胎在体外序贯培养系统中的发育效果。

Culture medium, water volume, root exudates of different crops, temperature, illumination affecting germination conditions of oospores of Phytophthora sojae studied in this reasch and compared the difference of germination of oospores of different race of Phytophthora sojae, filtrated the higher germination rate of strain and suitbable conditions combination of germination of oospores; The pure-line(Race 1) in virulence was obtained through isolation and culture and virulence identify of the second generation. The pure-line was analyzed by molecule method of ISSR. The results were summarized as follows:There was quite difference of germination rate of oospores on different culture medium.

本文测定了不同培养基、水量、农作物根渗出液、温度、光照对大豆疫霉菌卵孢子萌发影响，比较了大豆疫霉菌株间卵孢子萌发的差异，筛选出萌发率较高的菌株和适于卵孢子萌发的条什组合；通过继代单卵孢株分离培养和毒性鉴定，建立了大豆疫霉菌单卵孢株毒性纯系，并分析了单卵孢株毒性纯系后代群体ISSR分子指纹，结果如下： 1。

ResultThe best formula of the solid culture medium was ascertained to be castanea spp particle contents 70 g, corn flour contents 35, gypse contents 2 g, white sugar contents 1 g; the best formula of the liquid culture medium have been ascertained to be corn flour 4.2 g/100ml , castanea spp juice 5 g/100ml, dissolve for oxygen condition static culture, not being put into rocking bed.

结果固体培养基的最佳配方为栗木屑含量70 g，玉米粉含量35 g，石膏含量2 g，白糖含量1 g；大白栓菌液体培养的最佳条件是玉米粉4.2 g/100 ml，栗木汁5.0 g/100 ml，溶氧条件为静置培养。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。