查询词典 culture-medium

Strains D1 and D2 have the highest lipolytic activity in culture supernatent, great amounts lipase produced by remainder strains was located on the outer membrane of the cell envelope and was not released into the culture medium .

在摇瓶发酵水平上进行产酶优化试验，确定D2产脂肪酶的最佳培养方案为：培养温度9℃，摇床转速180r/min，培养时间40h，以2216为基础培养基，5g/l牛肉膏做碳源，6g/l氯化铵做氮源，分别替代酵母膏和蛋白胨。

The greatest efficiency in Agrobacterium-mediated transformation of lettuce cotyledons(2～3 days-old) was obtained by 10-time dilution of Agrobacterium cells, 15-min inoculation time,1-day pre-culture,4-day co-culture and without AS using the MS(Murashige and Skoog\'s,1962) medium supplemented with 0.2 mg/L 6-benzylaminopurine,0.1 mg/Lα-naphthaleneacetic acid,100 mg/L Kan and 500 mg/L Carb,.

在农杆菌介导的皱叶生菜子叶(2～3 day苗龄)转化试验中，采用含BA 0.2mg/L，NAA 0.1 mg/L，Kan 100 mg/L，Carb 500 mg/L的MS培养基，最优的转化条件为农杆菌稀释倍数为10倍，预培养时间为1 day，共培养时间为4 day，浸染时间为15 min，不添加乙酰丁香酮。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞，通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色，比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

Pyriformis cells cultivated in liquid complex culture media containing 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine were plotted by recording the number of cells present in the culture medium during the course of a 52-hour cell growth experiment, with a strictly controlled time interval of 4 hours.

本实验利用倒置光学(来源：A83B38f6C论文网www.abclunwen.com)显微镜下血球计数板计数法进行细胞计数，每两次细胞计数之间的时间间隔为4小时。

The effects of culture conditions,including carbon source,nitrogen source,mineral salt,temperature,pH,culture time and medium volume,on the production of mycelium by submerged fermentation of Russula vinos were investigated.

研究了正红菇在发酵过程中菌丝体及胞外多糖产量的变化趋势，碳源、氮源、无机盐以及发酵温度、pH值、时间和装液量等因素对正红菇液体深层发酵菌丝产量的影响。

The variation in production of mycelium and extracellular polysaccharide during the fermentation of Russula vinosa was studied. The effects of culture conditions, including carbon source, nitrogen source, mineral salt, temperature, pH, culture time and medium volume, on the production of mycelium by submerged fermentation of Russula vinos were investigated.

研究了正红菇在发酵过程中菌丝体及胞外多糖产量的变化趋势，碳源、氮源、无机盐以及发酵温度、pH值、时间和装液量等因素对正红菇液体深层发酵菌丝产量的影响。

The purpose of the paper was to establish the tissue culture and propagation technique system of Malus Teli. The shoot segments of Malus Teli as explants were cultured in vitro with MS culture medium and sterile system was established.

以MS为基本培养基，以观赏性小苹果&特丽&的茎段为外植体，建立了无菌培养体系，研究了不同的激素配比和不同茎段类型对增殖培养的影响以及不同的生长素组合对试管苗生根的影响。

Then Vero cells were inoculated with a final cell density of 1×105/mL . After 7 days of culture, cell density can reach 6~7×106/mL, then rabies virus VaG strain was inoculated with a MOI of 0.01. After 24h inoculation, the cultured viruses were harvested continuously for 12 days. The titer of collected virus in the culture medium was about 6.0 to 8.5 logLD50/mL. From these collected viruses, vaccines were prepared through the steps of concentration, inactivation and purification, The quality of these vaccines meets "The Chinese Requirements for Biologics Year 2000".

在5L体积的生物反应器中，加入含10g/L微载体的199培养基，接种Vero细胞至细胞浓度达到1×105/mL，培养7d后细胞可生长至6~7×106/mL，然后以感染复数为0.01接种狂犬病毒VaG株，接毒后24h开始收获，连续收获12d左右，收获的病毒滴度范围在6.0~8.5logLD50/mL，收获的病毒原液经浓缩、灭活和纯化等步骤制备成疫苗，各项质量指标均达到《中国生物制品规程》2000年版要求。

2DRG co-cultured with sciatic nerve undergoing Wallerian degeneration for 14days in vivo in serum free DMEM/F12,1sciatic nerve segment was added into co-culture system at the first moment,the axons were strong and straight and could adhered to each other,and the number of axons dramatically reduced compared with that of DRG cultured alone in serum free medium.2DRG cultured in serum free DMEM/F12for 4days,then the sciatic nerve segment was added into the culture system for 3days,the axons became straight and part of them adhered to each other.

分别在无血清和有血清的条件下将新生鼠的背根神经节和体内变性14天的大鼠坐骨神经段进行联合培养作为实验组，并以在相应条件下单独培养的DRG的轴突的生长情况作为对照组来观察比较轴突的生长情况。

Then, the relationship of the time for culture to its flocculability and the distribution of the flocculating activity in culture medium was studied.

研究了微生物产生菌BS-5培养时间与其培养液絮凝性能的关系，培养液中絮凝活性的分布。

However it can often be frust ating for a child when people are unable to pronounce their name.

但它往往可以令人沮丧的一个孩子当人们无法断言他们的名字。

All these compounds were obtained from this genus for the first time. Compound, 4-cumylphenol, was a new nature product.

所有化合物均为首次从本属海藻中分离得到，其中4cumylphenol为新天然产物。