查询词典 culture-medium

Inoculate a single colony in BMGY medium, grow at 30 °C with 250 r/min until culture reatch an OD6oo=2.0. Harvest the cells by centrifuging at 1,500 r/min for 5 min at room temperature. Resuspend cell pellet to an OD6oo=10 in BMMY medium to start induction.

将经过酶切鉴定和测序正确的阳性克隆电转入毕赤酵母GS115中，挑取酵母转化单菌落，接种于BMGY培养基，30℃、250r/min培养至OD_(600)=2.0时室温1，500r/min离心5min，用BMMY培养基重悬菌体使OD_(600)=1.0，重新放入摇床进行诱导。

Taxus cell lines were cultured on fresh medium succeedingly at regular intervals andantioxidant such as PVPP was added into culture medium in order to prevent browning of taxuscultured cells.

3红豆杉离体培养细胞在增殖过程中，自身富含的酚类被氧化成醌类物质，导致细胞褐变甚至死亡，在与农杆菌共培养时褐变现象更为严重。

ColipET32/IL-11/BL(DE21 recombinant human interleukin-11 in the flask were studied by orthogonal experiment design,including the of culture medium(glucose,tryptone,yeast extract and pH),shaking speed,medium volume and inoculum.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响，利用正交实验设计，对工程菌的生长条件和人白细胞介素-11融合蛋白表达进行优化。

Coli pET32/IL-11/BL(DE21 that expressed recombinant human interleukin-11 in the flask have been studied by orthogonal experiment design, including the components of culture medium (glucose, tryptone, yeast extract and pH), shaking speed, medium volume and inoculum concentration.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响，利用正交实验设计，对影响工程菌的生长及重组人白细胞介素11融合蛋白的表达因素如培养基中的葡萄糖浓度、蛋白胨浓度、酵母提取物浓度、无机盐浓度、pH及摇床转速、装液量、接种量等在摇瓶中进行了初步的研究。

Beijing DELING International Culture Media Limited Company is an integrative professional media company, which focuses on medium publication, internet marketing, animation industry, medium deputizing, project planning and executive conference organizing.

北京德领国际文化传播有限公司是以媒体出版、网络运营、动漫文化、媒介代理、项目策划、高端会议组织、资本运营为经营方向的专业文化传播运营机构。

The results showed that MS medium was the optimal basic culture medium; the regeneration frequency of leaf was the highest , then etiolated internode, internode was the lowest , their highest regeneration frequencies were 74.1%, 51.9%, 42.6%, respectively. The optimal dark treatment time for leaf and internode was 3 weeks; however the optimal dark treatment time for etiolated internodes was 2 weeks.

结果表明3种外植体的最适基本培养基均为MS培养基；叶片的再生效果最好，其次为黄化茎段，茎段最差，最高再生频率分别为74.1%，51.9%，42.6%；叶片和茎段的最佳暗处理时间为3周，黄化茎段的最佳暗处理时间为2周。

Therefore textual 1 and 2 part difference to Quan Zhou of geography foundation, maritime affairs transportation and race believe in and medium the culture blend phenomenon to carry on some kind of discuss with set up a textual writing understanding prophet with medium the literature stalemated background.

因此本文的第一、二部分分别对泉州的地理基础、海事交通和民族信仰及中阿文化交融现象进行一番论述，以构建本文的写作认识先知和中阿文艺胶着的背景。

Finally indicated, the best seed raise conditions of T11-1 and O7-7 is: 100mL seed culture mudium in 200 mL triangle bottle ,16 hours in shake bed;It′s best component fermentation medium is: peptone 5.0,yeast extract 3.0, beef paste 2.0,xylose 60.0,K_2HPO_4 2.0,MnSO_4·4H_2O 0.04,MgSO_4·7H_2O 0.20,FeSO_4·7H_2O 0.05;The best fermentation condition is: 150mL fermentation medium in 250mL bottle,vaccinates 10 %, 48 hours in shake bed under 46℃;The output of L-lactic acid using T11-1 and O7-7 from corncob respectively were 31.30g/L and 30.88g/L,and the conversion rate respectively were 52.17% and 51.47%.

结果表明，T11-1和O7-7的最佳种子培养条件为：在200mL三角瓶中于盛装100mL种子培养基，摇床培养16h；其最佳发酵培养基组分为：蛋白胨5.0，酵母浸粉3.0，牛肉膏2.0，木糖60.0，MnSO_4·4H_2O 0.04，MgSO_4·7H_2O 0.2，FeSO_4·7H_2O 0.05，K_2HPO_4 2.0；最佳发酵条件为：250mL输液瓶装载150mL发酵培养基，接种10%，在46℃条件下摇床发酵48h；利用T11-1和O7-7进行玉米芯L-乳酸发酵，其L-乳酸产量分别为：31.30g/L和30.88g/L，转化率分别为52.17%和51.47%。

This can be done by adding 1 mL of not less than 10 3 dilution of a 24-hour broth culture of the microorganism to the first dilution (in pH 7.2 Phosphate Buffer, Fluid Soybean–Casein Digest Medium, or Fluid Lactose Medium) of the test material and following the test procedure.

方法如下：将1mL该微生物24小时肉汤培养物的10 3倍稀释液加入供试品的首次稀释液（于pH7.2磷酸盐缓冲液，液体大豆酪蛋白消化物培养基，或液体乳糖培养基中）。

If tissue culture medium is incubated in dialyzers and then rat dorsal root ganglion cultures are exposed to this medium, characteristic changes occur.

EO残留量主要是指EO灭菌后留在物品和包装材料内的EO和它的两个副产品氯乙醇乙烷和乙二醇乙烷。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。