查询词典 culture-medium

METHODS: The Chelerythrine was used as the experimental group with concentrations ranging from 24.4μg/ml to 390.6μg/ml prepared with BHI broth medium with contained 2% glucose, and BHI culture medium was used as the control group.

采用二倍稀释法，用含2%蔗糖的脑心浸萃液肉汤琼脂液体培养基，将白屈菜红碱稀释为从390.6μg/ml到24。

The biotechnology production efficiency of crocin II, which has anti-cancer activity, was improved in a solid-liquid two-step cell culture in which the callus cells obtained from the solid medium were transferred to the liquid medium.

为提高现代生物技术生产藏红花素的产率和效率，该研究采取固液两步培养法将在固体培养基中获得的藏红花愈伤组织转移到液体培养基中进行生物合成，结果显示生物反应器比摇瓶培养更有利于生物量的积累，也更有利于2号藏红花素的合成。

Be the result of attribute of this kind of culture, brought up home of a batch of China's successful medium and small businesses, but the survival that what we see is the opportunity that those good having only, namely somewhat home of factitive China medium and small businesses.

正是这种文化属性的结果，造就了一批中国成功的中小企业家，但是我们所看到的只是那些拥有好的机遇的幸存者，也就是有所作为的中国中小企业家。

The changes of total nitrogen and total phosphorus concentration in solutions with total saccharides released by two kinds of algae,the Hydrodictyon reticulatum in artificial culture medium and the microalgae in a medium whose nutrient is supplied by bed mud from lakes,were studied respectively.

分别研究了水网藻在人工培养液培养下，微藻在以湖底泥为营养源培养下，藻代谢总糖浓度与溶液中总氮浓度、总糖与总磷浓度随培养时间的变化情况，结果表明两两均呈现一种反向变化关系。

Using the self made liquid culture medium JOA, we isolate Mycoplana and Rothia from the wasted medium oil of a large size transformer. The authors studied their morphologies, characters of physiology and biochemistry, the chemical composition of the cell wall.

用自制的 JOA液体培养基从报废的大型变压器介质油中分离得到枝动菌和罗思氏菌，并对其形态、细胞壁化学组分及一些生理生化特性进行实验分析。

Well developed shoots were successfully obtained after 45 days of culture of shoot tip explants on MS medium supplemented with 4-8 mg/l Benzylaminopurine and 0.5 mg/l α-Naphthaleneacetic acid. Addition of 0.01-0.5 mg/l Thidiazuron in the medium was found to be beneficial for rapid growth of shoot tip explants.

培植体培养於MS基本盐类并添加4-8mg/l BA和0.5mg/l NAA的培养基45天后，对促进新茎节萌发效果良好；而接种於含0.01-0.5mg/l TDZ固体培养之茎顶培植体，也显示有促进茎节快速生长的作用。

Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10％ heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法，分离32例乳腺癌患者外周血中的单个核细胞，在6孔培养板上(106/ml，2ml/孔)，用含10％热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究，建立的'新红星'苹果遗传转化体系为：叶片黑暗预培养3天，经农杆菌侵染3分钟，在含TDZ0.5mg/L，NAA0.3mg/L的MS培养基上黑暗共培养3天，然后加250mg/L Cef黑暗维持培养3天，再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现；转为光照条件1-2次的继代筛选，获得抗性芽后，进行扩繁。

METHODS: Femur and tibia were obtained from Wistar rats for primary culture of BMSCs. MSCs at passage 15-20 were firstly incubated in preinduction medium, for 24 hours, and then incubated in induction medium containing α-MEM, 2% dimethyl sulfoxide and 200 μmol/L butylated hydroxyanisole.

取Wistar大鼠股骨和胫骨，进行骨髓间充质干细胞原代培养，传至第15~20代融合状态的间充质干细胞置于预诱导培养基中培养24 h后，换用诱导培养基。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者，具有显著降低污染率的效果分别为60%及50%；克服内生菌污染而建立初代无菌培养之腋芽培植体，则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者，芽体再生率最高70%-75%，惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个；另在醣种类及浓度与抗生素试验，显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基，显著降低污染率至20%；但未污染之培植体，形态发生潜能低至40%以内，可反应生长之培植体芽体形成数也仅达1-1.2个之间，再生芽体之生长也显现抑制现象。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。