查询词典 culture-medium

METHOD Two in vitro experiments were performed for comparative study to evaluate the effect of extract, namely, adapting the addition of the contained medicine serum to culture medium and adding extract to the medium directly.

采用两种离体方法对比观察，即以家兔灌服该提取物后获取的含药物血清加至细胞培养基中的血清药理学方法和将提取物直接加至培养基中的直接体外给药法。

To establish the appropriate system for selection of Agrobacterium-mediated transformation of protocorm-like bodiesof Dendrobium nobile Lindl,materials were cultured in liquid paper medium,solid culture medium and liquid filter paper bridge,with Hyg at 7 different concentrations such as 0,20,40,50,60 and 80 mg/L.

为确立农杆菌介导法转化金钗石斛类原球茎的适宜的选择体系，利用液体纸基、固体培养基与液体滤纸桥3种培养方式，以0，20，40，50，60，80 mg/L质量浓度的潮霉素梯度试验，发现不同培养方式的选择效果有所差异。

No xylanase activity was found when the culture was carried out without xylan in the medium or with the saccharose as the only carbon source in the medium. Xylose inhibited the xylanase production.

发酵培养基中不含木聚糖时，米曲霉RIB128不产生木聚糖酶，蔗糖为底物时也不产生木聚糖酶，葡萄糖可以诱导产生少量木聚糖酶，木糖则抑制木聚糖酶的产生。

Results Fusarium sambucinum was the main pathogenic bacteria on C. deserticola stem rot which was first recorded; its suitable medium was PSA culture medium; among the carbon and nitrogen sources supplied, saccharose and peptone were most available; the temperature range of its growth was 10-30℃ and it grew best between 6-8 pH value. Duojunling, Junxianwei (1.5% methane dithiocyanate), Lüxiang No. 2 80% carbendazim, thiram, ziram, etc. were effective for controlling the pathogenic bacteria in laboratory.

结果 接骨木镰刀菌Fusarium sambucinum是肉苁蓉茎腐病的主要病原菌，为首次记录；适合其生长的培养基为PSA培养基，对碳和氮的吸收主要以蔗糖和蛋白胨为主，适宜生长温度为10~30℃，适宜生长pH为6~8；多菌灵、菌线威、绿享二号在室内可以有效地抑制病原菌。

Based on above results, the fermentation medium and cultivation conditions were investigated and the uniform design method was used in the optimization of fermentation medium. The techniques of fed-batch culture were also investigated in this paper, with this techniques acarbose fermentation titre increased from 1416 μg/ml to 2700 μg/ml, basically reached the level of industrial production.

在此基础上，又对发酵培养基的组成及发酵条件等方面进行考察，并采用均匀设计的方法对发酵培养基的组成进行了优化；对补料等发酵工艺进行了考察，使发酵单位由原来的1416μg／ml提高到2700μg／ml，基本上达到了工业化发酵的水平。

When the plantlet with induced roots were shifted to tuberous root inducing culture medium, the root obviously augmented which consequently led to the formation of test tube tuberous root. The optimized medium was MS+6-BA2mg/L+NAA 0.1 mg/L+sucrose 5%.

已生根的试管苗转入块根诱导培养基中，根部明显增粗，形成粉葛的试管块根，最佳培养基配方为：MS+6-BA 2mg/L+NAA 0.1mg/L+蔗糖5%。

The optimal medium consisted of 2% glucose, l.5% soluble starch, 2% peptone, 0.5% soya powder, 2.5% unpurified salt, initial pH 6.0~6.5. The optimum culture conditions was: 5% inoculation amount, seed cultural time 24h, 150mL liquid medium in 500mL Erlenmeyer flask, 29℃ and 180r/min for 3d.

对菌株B2817的发酵工艺进行了研究，得到其最佳的发酵培养基配方：葡萄糖2%，可溶性淀粉1.5%，蛋白 2%，黄豆粉0.5%，日晒盐2.5%，pH6.0~6.5。

Two culture methods, sterilized MS medium and garden soil medium, were comparatively studied for spore germination of Dryopteris varia, with the observation of its gametophyte development under light microscope.

应用无菌培养和常规泥土培养两种方法对变异鳞毛蕨孢子进行了比较研究，并在光学显微镜下观察了其配子体的发育过程。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。