查询词典 culture-medium

With the corm and scale of A. albus in Zigui County in Hubei Province as tested material, MS was used as the basic medium with different hormones to respectively match 10 kinds of media for induction, differentiation for tissue culture of A. albus The induced clumpy buds were cut into single plants and were put into the rooting medium of MS+NAA 0.1-0.5 mg/L and the effects of different hormone matching on callus formation, bud differentiation and rooting were studied.

方法］以湖北省秭归县的花魔芋球茎和鳞片作为外植体，以MS培养基为基本培养基，添加不同激素，分别组配成10种诱导培养基和分化培养基进行组织培养，然后将诱导的魔芋丛芽切成单株后接入MS+NAA0.1~0.5 mg/L生根培养基，研究不同激素配比对愈伤组织形成和芽分化以及生根的影响。

The cells were preincubated at first in liquid phase for 24 hours at a concentration of 5×106 nucleated cells/ml of RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and concanavalin A5μg/ml of culture medium.

先将脾脏有核细胞(5x10~6/ml)在含有ConA(5μg/ml)的液相中培养24小时，然后收集非粘附细胞，将其接种到含0.88％甲基纤维素、10％FCS，ConA 5μg/ml和15％ConA-LCM的半固体系统中培养，细胞的终浓度为5x10~5/ml。

The results showed that transformed buds could be obtained after the epicotyl segments were cultured on co-culture medium (MS+3mg/L BA) for 3 days and then on selective medium with 100 mg/L Kanamycin and 500 mg/L Cefotaxime for 2 weeks. The transformation efficency of Sweet orange with the chit42 and phyB genes reached 27.8% and 35.6%, respectively.

上胚轴经农杆菌工程菌液浸染后，在共培培养基(MS+3mg/LBA)上培养3d，然后分别转移到含有100mg/L Kan（卡那霉素，Kanamycin）和500mg/L Cef（头孢噻肟霉素，Cefotaxime）的选择培养基上培养14d后，上胚轴开始分化抗性芽，chit42基因和phyB基因的抗性芽再生率分别为27.8%和35.6%。

When culture condition was changed abruptly from LES medium to RPMI-1640 medium, different reproductive mode including binary fission, ternary fission,multiple fission and gemmation were found after growth retardation of 24 ～ 72 hours. This phenomenon continued less than 24 hours and binary fission remained reproductive mode then.

当从LES培养基突然变为RPMI-1640培养基时，人芽囊原虫出现短时间(48～72h)内生长停滞，随后出现多种形式的繁殖方式，可见较多的二分裂、出芽生殖、三分裂、多分裂、裂体生殖，持续24h后恢复为二分裂为主。

This protocol incorporates treatment of early differentiating embryoid bodies with actiin A to enhance the specification of the endodermal germ layer, followed by adherent culture in serum-free medium and the final application of a commercial, lung-specific medium.

这一个记录包含（a 用活化素A提高早期分化中的胚状体向内胚层的分化（b在无血清培养基中进行黏附培养和应用商业化的肺特异性的培养基。

To isolate forebrain and hindbrain of human fetuses about 9 weeks and 12 weeks from spontaneous abortion. Neural stem cells were cultured in special culture medium and were compared with growth characterization.The differentiation from these neural stem cells was investigated in both a fetal bovine serum-containing and a non-supplemented,serum-free basal medium.

材料与方法：利用神经干细胞的条件培养基，对从9周和12周胚龄的自然流产胎儿分离的前脑、后脑进行神经干细胞培养，比较其生长特性，并分别观察在无生长因子的基础培养基以及含有胎牛血清的培养基中神经干细胞的分化情况。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化，用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

Since reforming and opening up, it is clear that form convergent, architecture convergent and culture convergent in China. The problem of town individuated has became a hot issue. Depend on the issue "Study on town individuated location in planning of medium-small towns "that directed by Professor Cheng Daoping in Shandong Normal University, this paper choose "Study on Individuation Measurement of Medium-Small Towns" for research, the purpose is that study on individuate level with quantitative method, is an integral part of the subject and important foundation for positioning of individuate towns.

改革开放以来，我国城镇发展中形态趋同、建筑趋同、文化趋同现象明显，城镇个性化问题成为近年来城市研究的热点问题，籍于山东师大程道平教授主持的"中小城镇规划中的城镇个性化定位研究"课题，本文选择"中小城镇个性化测度研究"为题进行研究，目的是为了从定量的角度研究城镇个性化水平，作为课题的有机组成部分，为定位研究提供基础。

And on the basis of Grace medium, different mixes of Fetal Bovine Serum, MM medium, Hydrolysate lactalbumin and Yeast extract was developed on different levels to culture these tissues and cells.

本实验以灰飞虱的四种组织：中后期胚胎、若虫头部组织、成虫的精巢和卵巢为材料进行了培养实验。

Mixed anther of seven alfalfa varieties or Strains (MT-2, MT-146, MT-328, MT-335, Xinmu No.1, Xinjiang Daye and KS220-97) were inoculated on the double layer medium which contained N6 macroelement, MS microelements, MS molysite, B5 organic compound, 2,4-D 2 mg/L, 6-BA 0.5 mg/L, and induced callus, then the callus was induced on the medium of the different composition and the additives, to observe its result of culture.

将7种苜蓿品种（MT-2、MT-146、MT-328、MT-335、新牧1号、新疆大叶和KS220-97）的混合花药接种于N6大量元素＋MS微量元素＋MS铁盐＋B5有机物＋2，4-D 2mg/L+6-BA 0.5 mg/L的固液双层培养基上，诱导出愈伤组织，再转接到不同成分及添加物的培养基中培养，观察培养效果。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。