查询词典 culture-medium

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖，结果表明：高约2cm的无菌苗在MS+0.6mg／L 6-BA+0.1 mg／L NAA或MS+0.4～0.6mg／L ZT+0.1 mg／L NAA培养基上培养15 d左右可诱导出不定芽，分化频率为100％，平均每株产芽35.7个；MS+0.2mg／L 6-BA+0.1mg／L NAA+10mg／L AD培养基有利于芽伸长；生根培养基为1／2MS+0.4 mg／L NAA+0.2mg／L IBA时，生根率可达75％。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Picking out single colony from ammonifying culture medium solid medium and seeding them in nitrosation culture medium to culture and purify to obtain heterotrophic nitrifying bacteria.

从氨化培养基固体培养基中挑取单菌落，接种于亚硝化培养基中培养、纯化，获得异养硝化细菌。

Results The pyronaridine-coated plate and culture medium were proved to be stable. The effective period of pyronaridine-coated plate, the ampule sealed liquid culture medium and the bottled lyophilized culture medium, all stored at 4 ℃ was 6 months, 2 months and 2 years respectively.

结果 研制的咯萘啶涂药板及现场用的培养基效果稳定，咯萘啶涂药板4 ℃贮存有效期为6个月，4 ℃贮存的安瓿封装液体培养基及瓶装冰冻干燥培养基有效期分别为2个月和2年。

In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明，嗜热链球菌在营养丰富的培养基中的生长延滞期缩短，到达对数生长顶点的时间变短，稳定期的时间延长，当经过二次培养后，嗜热链球菌几乎不经过延滞期直接到达对数期，平稳期持续的时间较短，很快到达衰亡期；嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系，菌数多，其代谢旺盛，菌数降低其代谢速度减慢；嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化，并可能存在着细胞凋亡，与菌体增殖和产酸规律密切相关，随着培养时间延长，培养基中营养物质变得贫乏，菌体形态变化和细胞凋亡的情况愈加明显。

The medium used is a modified artificial medium for growing Volvox globartor, devised by Provasoli and Pintner (1959). The process of culture is divided into three steps:(1) preliminary culture;(2) pure culture and (3) expand culture.

我们采取过渡性的初步培养、纯化培养和扩大培养三步法，利用光强度800—1200勒克斯，每日照光10—12小时，一般室温在15—22℃(冬季不低于9℃，夏季不高于28℃)下，细胞已完全能正常的生长、繁殖而被培养成功。

The results indicated:0.1 mg/L 2,4-D or 0.1 mg/L 2,4-D+0.2 mg/L KT was optimal to induct the callus of carrot hypocotyls.The majority of the callus induced on the medium containing 0.1 mg/L 2,4-D regenerated embryoid,whereas,the callus induced on the medium containing 0.1 mg/L (2,4-D)+0.2 mg/L KT produced adventive buds.During the growth of regenerated plant,MS medium is available for rooting of seedling,and seedling regenerated on B5 medium must be placed on the B5 medium containing 0.1% IBA to develop root.

试验结果表明：0 1mg/L2，4 D或0 1mg/L2，4 D+0 2mg/LKT的激素组合有利于胡萝卜下胚轴的愈伤组织诱导；在0 1mg/L2，4 D的培养基上形成的愈伤组织主要以胚状体的形式再生，而在含0 1mg/L2，4 D+0 2mg/LKT激素组合的培养基上形成的愈伤组织主要以发生不定芽的方式再生；再生过程中，B5基本培养基上的苗子不易生根，需要附加0 1mg/L的IBA，而在MS培养基上苗子可直接形成根。

A first recording medium (1) containing a content encrypted by a content encryption key, a portable second recording medium (2), a terminal (10) to which the first recording medium and the second recording medium can be detachably attached, a communication device (20) to which the second recording medium can be detachably attached, and a server (40) connected to a communication device.

配备存储用内容密钥加密的内容的第1存储介质(1)、可搬运的第2存储介质(2)、可装卸第1存储介质和第2存储介质的终端(10)、可装卸第2存储介质的通信装置(20)、以及连接通信装置的服务器(40)，终端将用公开钥对通信钥和持有人ID加密后的第1加密数据写入第2存储介质，安装于通信装置，并送到服务器。

In the spiral pipe heat exchanger exports terminal, the ammonia steam temperature has been heated up 85 ℃--95 ℃, by now, the ammonia steam had the 45kg-55kg/cm2 ammonia steam pressure, sent in the ammonia steam turbine through the constant temperature pipeline, impelled ammonia steam turbine revolving, led the generator electricity generation;After the ammonia steam makes the merit release energy, the temperature drop, the returns ammonia storage tank, passes through again adjusts the press pump to press into the spiral pipe-type heat exchangers to carry on the next circulation;Including the ammonia steam turbine entire ammonia steam road is becomes the independent closed cycle system, is isolates completely with the outside air;The ammonia steam only plays the carryhome and the shift energy role, in the electricity generation process does not consume the actuating medium, the stored energy carrier water also is only gets up the carryhome and the shift energy function,The waterway also is from becomes the independent closed cycle system, in the electricity generation process also the needless water consumption, through the actuating medium ammonia steam and the stored energy carrier water unceasing shuttle service, transforms through the heat interchanger the solar energy as the actuating medium ammonia steam heat energy and the kinetic energy,With the aid of the ammonia steam turbine heat - machine transformation function and the generator machine - electricity transformation function, has realized the solar energy hot - electricity entire conversion process, transforms continuously the solar energy into the electrical energy, power supply for foreign;The low temperature generating system must solve three big technical keys:One, the anticorrosion (has actuating medium has strong corrosiveness), two is Explosion-proof (Some actuating medium Can have the detonation with the air mix when divulging, controls warm malfunction, elevates temperature suddenly also can have detonation), three is guards against the revolution axis divulging

以太阳能低温发电系统为例，低温发电方法是这样进行的：以水作为储能载体的太阳能采集器将太阳能采集起来，将水温提升至85℃--98℃；用氨作为工作介质储于氨储罐及氨汽路中；采用螺旋管型热交换器；用调压泵将氨储罐中的氨汽压入螺旋管型热交换器的螺旋管内，用调压泵将携带太阳能的85℃--98℃的储能载体热水压入螺旋管型热交换器的螺旋管外壁空间进行循环式热交换，在热交换器的螺旋管出口端，氨汽温度已被加热到85℃--95℃，这时，氨汽具有45kg—55kg/cm2的氨汽压力，通过恒温管道送入氨汽轮机，推动氨汽轮机旋转，带动发电机发电；氨汽作功释放能量后，温度下降，返回氨储罐，再经调压泵压入螺旋管型热交换器进行下一次循环；包括氨汽轮机在内的整个氨汽汽路是自成独立的封闭循环系统，与外界空气是完全隔绝的；氨汽只起携带和转移能量的作用，发电过程中并不消耗工作介质，储能载体水也是只起携带和转移能量的作用，水路也是自成独立的封闭循环系统，发电过程中也不消耗水，通过工作介质氨汽和储能载体水的不断循环运行，通过热交换器将太阳能转化为工作介质氨汽的热能和动能，借助氨汽轮机的热—机转化功能和发电机的机—电转化功能，实现了太阳能的整个热—电转化过程，将太阳能源源不断地转变为电能，对外供电；低温发电系统要解决的三大技术关键：一是防腐（有的工作介质具有较强的腐蚀性）、二是防爆（有的介质泄漏与空气混合会产生爆炸，控温失灵，急剧升温也会产生爆炸）、三是防轴漏（汽轮机是动态旋转体，必须解决工作介质的防轴漏问题）。

The inducement medium was as follows respectively: for the leafstalk, B5 medium with 6-BA 0.5 mg/L+IBA 0.1 mg/L, and for stems, B5 medium with 6-BA 1.0 mg/L+IBA 0.2 mg/L, and subculture medium both were B5 medium with 6-BA 1.0 mg/L+2, 4-D 0.1 mg/L.

利用叶柄和茎获得松散愈伤组织时，其诱导培养基组成分别是：B5培养基+6-BA0.5mg/L+IBA0.1mg/L和B5培养基+6-BA1.0mg/L+IBA0.2mg/L，其继代培养基均为B5培养基+6-BA1.0mg/L+2，4-D0.1mg/L。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。