查询词典 culture-medium

The present paper carries on the raise first to the camptotheca acuminata roots of the hair, then in the use chloroform extraction camptotheca acuminata culture medium the camptotheca acuminata alkali and so on effective component, and examines the law with HPLC to examine its effective component, the examination discovers the camptotheca acuminata secretion in the culture medium secondary metabolite besides the camptotheca acuminata alkali, the hydroxyl camptotheca acuminata alkali, the white birch fatty acid, but also includes other effective components.

本论文先对喜树发根进行培养，然后使用氯仿提取喜树培养基中喜树碱等有效成分，并用HPLC检测法检测其有效成分，检测发现喜树分泌在培养基中的次生代谢产物除了喜树碱、羟基喜树碱、白桦脂酸外，还含有其他有效成分。

Thepresent paper carries on the raise first to the camptothecaacuminata roots of the hair, then in the use chloroform extractioncamptotheca acuminata culture medium the camptotheca acuminataalkali and so on effective component, and examines the law withHPLC to examine its effective component, the examination discoversthe camptotheca acuminata secretion in the culture medium secondarymetabolite besides the camptotheca acuminata alkali, the hydroxylcamptotheca acuminata alkali, the white birch fatty acid, but alsoincludes other effective components.

本论文先对喜树发根进行培养，然后使用氯仿提取喜树培养基中喜树碱等有效成分，并用HPLC检测法检测其有效成分，检测发现喜树分泌在培养基中的次生代谢产物除了喜树碱、羟基喜树碱、白桦脂酸外，还含有其他有效成分。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系：选取生长20d左右的健壮的小黑杨组培苗叶片，通过主叶脉剪2～3下，预培养3～4d；将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3～0.4浓度，进行浸染；侵染时间为6～15min；共培养3d后，转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

Result］ The new buds and petals of I.germanica was proper to be used as explant of tissue culture, with lower browning rate compared with other positions. The well situated tissue block of explant (3 mm×3 mm×3 mm) might reduce browning rate. Adding 0.1%Vc or 0.3% active carbon in medium had obvious effect on preventing tissue browning. The exogenous hormone 2 mg/L 6-BA was easy to lead to brownness turning, and 2 mg/L Kt and 2 mg/L NAA brought less effect to tissue browning. When using disinfectants of alcohol, its dipping time of 5～10 s for explant was the best, with the minimum browning rate. The culture under full illumination condition was liable to induce brownness turning, compared with other cultured conditions, the brownness turning could be restrained effectively by using imitating artificial climate condition for culture.

结果］ 鸢尾的新生芽和花瓣较适宜作组织培养的外植体，与其他部位相比褐变率较低；外植体组织块适中（3 mm×3 mm×3 mm）可降低褐变率；在培养基中加入0.1%Vc或0.3%活性炭对防止组织褐化效果明显；外源激素2 mg/L 6-BA易引发褐变，2 mg/L Kt和2 mg/L NAA对组织褐变影响不大；使用乙醇消毒剂时，对外植体浸泡时间以5～10 s为佳，褐变率最低；全光照条件培养容易诱发褐变，采用仿人工气候条件培养较其他条件培养可有效抑制褐变的发生。

At the same time, South Korea formed capitalistic thought and culture by accepting Christianity as the medium of the nations traditional culture and western culture.

与此不同，韩国是以基督教为民族文化与西方文化的媒介，便形成了自己资本主义的思想文化。

Additional, it is natural resources of dish of the vivid land that put an amount, raise capital to use efficiency, combine the strategic disposition that industry of culture of the Chengdu City grows, and the whole of area of garden of industry of river of Chengdu bright and beautiful develops a program, what rich luck transmission plans to make be a target in order to collect enterprise of culture medium originality and resource is muti_function business affairs center, construction is compositive industry of movie and TV, ad industry, publication industry, presswork industry the platform of culture originality industry that wait, temporary denominate is the project " originality Chengdu ".

另外，为盘活存量土地资源，提高资金使用效率，结合成都市文化产业发展的战略部署，以及成都锦江工业园区的整体发展规划，博瑞传播拟打造一座以聚集文化传媒创意企业和资源为目标的多功能商务中心，建设集成影视产业、广告产业、出版产业、印刷产业等的文化创意产业平台，项目暂时定名为"创意成都"。

The opotimal experiment showed that the optimal culture medium contented lotus root knurl∶lotus root peel=1∶1, water 1.88mL/g, KH2PO45.63%, cotton cypress3.75%, corn powder 2.50%, and optimal culture condition was 27℃, strain age 2days, inoculum 10%, culture time 10days.

对发酵工艺条件进行优化，最优培养基配方为：藕节：藕皮(1∶1)，水1.88mL/g， KH2PO45.63%，棉粕3.75%，玉米粉2.50%，最优培养条件为：温度27℃，种龄2天，接种量10%，培养时间10天。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

Impact of PCL capsulation on the mesenchymal stem cells activity studied in vitro studied,one plate culture with complete culture medium for control group and three encapsulated culture for experimental groups installed.

以添加10%胎牛血清的完全培养液平皿法细胞培养为对照组，设置3个实验组进行聚己内酯囊管化骨髓间充质干细胞培养活性研究。

Methods: hMSCs were separated from human marrow with Ficoll Paque reagent or direct culture and expanded in different culture medium with newborn bovine serum. The proliferation and growth characteristics were observed in primary and passage culture.

采用Ficoll Paque淋巴细胞分离液和全血接种两种方法分离成人MSC，筛选体外培养hMSC适合的培养基和适宜的血清含量，流式细胞仪检测hMSC表面抗原表达。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"