查询词典 culture-medium

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

MS + NAA0.2 mg · L-1+6-BA2 mg · L-1 is better culture medium for shoor growth,and MS+NAA0.1 mg · L-1 is better root initiation culture medium.

栀子再生芽的生长以MS培养基加入NAA0.2mg·L~(-1)+6-BA2mg·L~(-1)为佳，而根的形成和生长以培养基MS+NAA0.1mg·L~(-1)为佳。

The results showed that the mycelial could grow better on the most of culture media, but worse on the Rose Bengal Medium culture medium than others. The temperature ranged for mycelial growth was 5~25℃, 20℃ was the optimum. The mycelial might grow from pH 2.5 to pH10, pH5 was the best. The effect of light on the mycelial growth was not evident and mycelial lethal temperature was 49℃, 10min. The sclerotia could germinate in the most kinds of nutrition.

试验结果表明：病菌菌丝在大多数培养基中均能良好生长，而在孟加拉红培养基上生长较为缓慢；菌丝在5~25℃范围内均能生长，最适温度为20℃；菌丝生长的pH值范围为2.5~8，最适pH值为5；光照对菌丝生长影响不大；菌丝致死温度为49℃、10min。

At first, we investigated the optimal components of culture medium for thereafter purification. We found that the optimal culture medium could produce better yield of red-pigmented antibiotic when 1.5% fructose and 1.5% malt extract included, and incubated at 30℃ under shaking conditions for 6 days.

我们逐步由探讨培养基条件为始，以利后续的生产纯化的进行，发现以1.5%果糖与1.5%麦芽抽出物之液态培养条件下，以30℃震荡培养6天后，有最适红色抗菌物质之产率，并且可减少其他色素的产生，再试著将其纯化。

The influences of culture conditions, such as medium compositions (including carbohydrate, nitrogen, mineral salts, amino acid and stimulus), medium pH, temperature, culture time, inoculation amount and aeration, on the growth and antimicrobial activity of Bacillus natto were evaluated.

培养基的组成和培养条件对纳豆菌生长及抗菌物质的产生有很大的影响。

Methods:Specimens were inoculated on sabouraud culture medium and that on CHROMagar Candida at the same time, then the yeast fungus which grown on sabouraud culture medium was identified by API20C AUX, while that on CHROMagar Candida was judged directly according to the color and appearance of colonies .

将标本同时接种沙氏基和CHROMagar念珠菌显色培养基，沙氏基上生长的酵母菌经API20C AUX酵母菌鉴定系统鉴定，CHROMagar念珠菌显色培养基根据菌落颜色、形态直接判读。

The methods of sticking tissues piece to culture primary hFCECs and mouse 3T3 fibroblast conditioned medium to culture passage hFCECs were established;2.The Proliferative capacity of corneal limbus hFCECs is better than central hFCECs.3.3T3 conditioned medium could markedly promote the proliferation of epithelial cells

结论一、组织块贴壁法原代培养人胎儿角膜上皮细胞以及小鼠3T3成纤维细胞条件培养液传代培养方法基本建立；二、人胎儿角膜缘部上皮细胞增殖能力优于中央部；三、3T3细胞条件培养液能促进角膜上皮细胞的增殖。

Results: Compared with I/R+insulin group and I/R+pioglitazone group. the ultrastructure of cardiomyocytes suffering ischemia reperfusion injury recovered markedly, the fluorescence intensity of the intracellular calcium ion decreased, the survival rate of cardiomyocytes increased, LDH, CK, MDA concentration in culture medium decreased and SOD concentration in culture medium increased in I/R+insulin+pioglitazone group.

结果：吡格列酮与胰岛素联合应用可提高缺血再灌注损伤心肌细胞的存活率，降低细胞培养液中的LDH、CK和MDA含量，提高SOD的含量，降低心肌细胞钙离子染色的荧光强度，部分恢复心肌细胞的超微结构，与单独应用吡格列酮或胰岛素相比，具有统计学意义。

The fermentation culture media for pNSR32/BL21(DE3) was investigated with shake culture method.Based on M9 medium and for the induction of lactose ,so as to determine the optimal concentration of ingredients of medium.

在M9培养基的基础上，以乳糖为诱导剂，对基因重组蛛丝蛋白工程菌pNSR32/BL21(DE3)的发酵培养基进行了优化。

Optimization technology was acetidin: N-hexane (3:7) as low-pole solvent system, methanol: dichloromethane (1:9) as polar solvent system, 20% glycerol filter paper as moisturizer, 14 h age Bacillus subtilis as indicating strain seed on thin layer plate cultivated for 14~18 h, 1% TTC staining time 1~2 h, bacterial mix method suitable to 0.2%~0.6% agar semisolid bouillon culture-medium, bacterial spread method suitable to 1% agar semisolid bouillon culture-medium.

结果：该方法展开系统筛选简单，抑菌斑位置准确、斑点清晰，其优化条件为：以乙酸乙酯：正己烷(3：7)为弱极性展开系统，甲醇：二氯甲烷(1：9)为强极性展开系统，用20%甘油滤纸对湿室保湿，以14h菌龄的枯草芽孢杆菌为指示菌种子液，自显影薄层板36℃培养14~18h，1%TTC显色时间为1~2h，含0.2%~0.6%琼脂的半固体肉汤培养基应使用混菌法生物自显影，含1%琼脂半固体肉汤培养基应使用涂布法生物自显影。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"