查询词典 culture-medium

Two specieses, Lycoris radiate and Lycoris aurea, were selected for the studying of artificial propagation in vitro. A few specieses of Lycoris, from the southeast area of China and part of locations in Japan, were chosen to research their karyotype differentiation and to measure their genetic diversity by ISSR analysis. The results could be summarized as follows.1. The vegetative propagation conditions of Lycoris aurea and L. radiate in vitro was studied by two-scaling. Different illuminate condition had an effect on the bulblet formation rate. To the species of L. radiate, the rate was higher in the state of 16h 800-12001x illumination than in the darkness. The medium also affected the new bulblet formating rate. When the culture was MS medium 0.2 mg/L NAA 4 mg/L 6-BA, the bulblet formationg rate of L. aurea was 220%;at the same time, when the medium was MS 0.2 mg/L NAA 2 mg/L 6-BA, the rate of L.

本研究选择西南部分地区及日本的几个石蒜品种，从细胞学和DNA分子角度，分析了它们的遗传多样性水平和遗传结构状况，对红花石蒜和忽地笑的离体繁殖技术进行了初步研究，得出如下结论： 1 进行了红花石蒜和黄花石蒜双鳞片快速繁殖条件的研究，结果表明：红花石蒜在16小时800-1200 lx光照下比黑暗条件下出芽率要高；黄花石蒜在MS NAA0.2mg／L 6-BA4mg／L下出芽率为220％，红花石蒜在MS NAA0.2mg／L 6-BA2mg／L下出芽率为108％；NAA比IBA有利于石蒜生根；硅藻土显著提高黄花石蒜双鳞片出芽率，活性炭起抑制作用；6％蔗糖浓度有利于红花石蒜小鳞茎增重，MS 6-BA4mg／L NAA0.5mg／L培养基有利于小鳞茎增殖，切割一刀比两刀有利于小鳞茎增殖。

N6 medium, MS medium and BN medium were adopted in tissue culture of Nipponbare Scutellum for performance comparison.

采用B6、MS和NB三种培养基，以粳稻品种日本晴的成熟胚为受体材料进行组织培养，比较了三种培养基的效果。

This paper studied on domestication of plantlets in tissue culture and rapid propagation of Dendrobium loddigesii Rolfe which dealed with the different medium composed of gravel, rotten soil, perlite, turves, sawdust and china clay, in the different light intensity and temperature. The results showed that the best selection of medium on the domestication was 10cm gravel in bottom, rotten soil + sawdust + perlite,(5:1:1) in the middle part ,5cm greenness bryophyte on the surface.the best selection of medium on the transplantaion of plantlets in vitro of bramble,and light intensity was employed harf overshadow by adumbral net,temperature confined 14℃－28℃.

本文研究了环草石斛组培苗炼苗的影响因素如光照强度，温度以及基质等，初步获得环草石斛组培苗炼苗生产技术：能进行人工光、温、水控制的如塑料薄膜大棚内进行环草石斛组培苗炼苗，光照强度的要求是使用遮阳网半遮阴；温度控制在14℃－28℃；出瓶苗要求株高2～5cm，叶片4～7，根数3～8；组培苗炼苗基质为底层是约10cm厚的碎石，在碎石上面是腐殖土+锯末+珍珠岩(5：1：1)，最上面是一层5cm厚的新鲜苔藓。

BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10%fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C).

通过改良的骨髓培养法分离成年大鼠骨髓间充质干细胞，对照组以基础培养液(10%胎牛血清+高糖DMEM+100U/L青霉素+100 U/L链霉素+2 mmol/L谷氨酰胺)培养，实验组用条件培养液(基础培养液，10 mmol/Lβ-甘油磷酸纳，10-7 mol/L地塞米松，50 mg/L维生素C)进行诱导培养。

This experiment was conducted with swamp buffalo to compare the effects of different acquisition methods, serum-free medium or serum-contained medium and serum-contained medium with many growth factors, on the culture of oviductal epithelial cells.

以广西沼泽型水牛输卵管为材料，比较研究不同取材方法、有无血清及生长因子对水牛输卵管上皮细胞培养的影响。

The results showed that the transformation efficiency of the leaves precultured for 9 d on preculture medium, infested in OD value equal to 0.7 bacterium medium for 10 min, and then co-culture in dark conditions and co-cultured medium with 100μmolL^(-1) acetosyringone for 3 d respectively; was optimal.

结果表明，预培养9d的悬铃木叶片在OD600值为0.7的根癌农杆菌菌液中浸染10min后，再在含有100μmolL^(-1)的乙酰丁香酮的共培养培养基上黑暗条件下共培养3d叶片的遗传转化效率最高。

The germination test of Sporocarps of Microlepia hancei prantl had been done, the callus can be induced from the delicate stem of Microlepia hancei prantl; The inducement and proliferation medium of callus was MS＋6-BA2 mg/L+NAA0.2 mg/L;the optimal combination of hormones of buds differentiation was MS+6-BA 0.4 mg/L; the effect of MS medium in radication culture was better than 1/2MS, the roots of plants were all efficiently induced in MS medium without reference to adding IBA、NAA or IBA＋NAA, the rate of radication was high; the effect of seedling cultured in the mixture of perlite and turf soil was better than perlite、garden soil and river sands.the mixture of perlite and turf soil has the excellences such as ventilate、water conservation 、nourishment and so on,the survival rate was higher and seedling substance was also hale.

试验进行了华南鳞盖蕨孢子果的萌发试验，并利用幼嫩茎段进行诱导愈伤组织；愈伤组织的诱导与增殖培养基为MS＋6-BA2 mg/L+NAA0.2 mg/L；其芽分化最佳激素组合为MS+6-BA 0.4 mg/L；生根培养中MS基本培养基比1/2MS效果好，MS基本培养基不论附加生长素IBA、NAA或IBA＋NAA，均能有效地诱导植株生根，生根率高；假植以珍珠岩和泥炭土的混合物为基质效果优于珍珠岩、菜园土和河沙，具有通气、保水、营养等优点，假植成活率高，苗势健壮。

Where sterility test media are to be used in the Direct Inoculation of the Culture Medium method under Test for Sterility of the Product to be Examined , modify the preparation of Fluid Thioglycollate Medium and the Soybean–Casein Digest Medium as follows.

当无菌检查培养基用于供试产品无菌检查项下的培养基直接接种法时，按如下内容变更巯基醋酸盐液体培养基和大豆-酪蛋白消化物培养基的制备方法。

The microorganisms was domesticated by adding a certain amount of lead in the soil, and three lead-tolerant strains including Pb-R-1, Pb-R-2 and Pb-H-3 accordingly originating from 300 mg/L, 750 mg/L and 900 mg/L lead medium were isolated with a series of PDA medium of different lead concentrations. Through the identification, Pb-R-2 was confirmed as Aspergillus sp., Pb-R-3 as Penicillium sp. The trials of transferring culture showed that all strains had the stable heredity of lead tolerance in different composition PDA medium.

通过向土壤中添加一定量的铅来驯化土壤微生物，然后利用一系列不同铅浓度的PDA培养基分离耐铅微生物，结果获得一株耐300 mg/L铅的细菌Pb-R-1和二株分别耐750 mg/L和900 mg/L铅的真菌Pb-R-2和Pb-R-3，通过鉴定得知，Pb-R-2菌株为曲霉，Pb-R-3菌株为青霉；不同成分培养基传代培养实验表明，3个菌株有稳定的耐铅遗传性；通过液体悬浮培养获得：Pb-R-1、Pb-R-2和Pb-R-3对Pb(上标 2+)的吸附率依次为35.5%、75.5%和48.2%。

Two weeks after of culture calli were initiated on the surface of sections. Light-yellow callus from one explants was selected and proliferated on LS medium. And then it transferrated to somatic embryo induction medium (LS basal medium with lmg/1 BA).

选择由一块切段产生的淡黄色愈伤组织，繁殖并连续选择几代至其外观稳定，转移到含1mg/1BA的LS培养基上，15天后即可观察到有大量的球形胚形成，但只有少数可按正常的胚胎发育途径发育。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"