查询词典 culture flask

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Result］ It could be concluded through analyzing the model that both the mass uncertainties of quinoline phosphomolybdate precipitates and tested samples were from the resolving power and precision of balance and its maximum tolerance in assay certificate; the uncertainty brought in by volumetric flask was from the permissible error of 250 ml volumetric flask, the operation of increasing the volume to scale of volumetric flask while diluting solution and the difference between experimental temperature and the temperature at the time when the volumetric flask was calibrated; the uncertainty brought in by pipette was from the permissible error of itself, the operation of increasing the volume to intake scale while taking solution and the difference between experimental temperature and the temperature at the time when the pipette was calibrated.

结果］磷钼酸喹啉沉淀和试样质量的不确定度均来自天平的分辨力、精确度、鉴定证书上天平的最大允差，容量瓶引入的不确定度来自250 ml容量瓶本身的允许误差、稀释溶液时将体积增加到容量瓶刻度的操作和试验温度与容量瓶校准时温度的差异，吸量管引入的不确定度来自于吸量管本身的允许误差、吸取溶液时将体积增加到吸取刻度时的操作和试验温度与吸量管校准时温度的差异。

NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中，进行斜面培养，培养温度28-37℃，培养时间2-6天；(2)种子培养：用无菌水将斜面上培养的孢子制成单孢子悬液，并接种到种子培养基中培养，温度为28～32℃，转速为200rpm，培养2-6天；(3)发酵培养：将种子液接种到发酵培养基中培养，温度控制在28-37℃，pH控制在6.0-9.0，转速180-220rpm，发酵5-9天，得到链霉菌，采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离，再采用树脂方法进行精制，获得多肽类抗生素安来霉素。

The author mainly discusses the relationship between the two culture phenomena: culture identification and culture uniform tendency, describes the culture identification in the minds of people in the period of culture globalization, detailedly traces the historical evolvement of culture identification, and expatiates the theory of cultural nationalism 3identification, strong culture identification and entirely westernization identification, at last objectively gives some opinions on the realistic function of western culture in the development of modern society. Meanwhile, the author does not echo what other says, and entirely opposes the what is called culture imperialism or western culture intimidation, on the contrary, the author impersonally analyses some other voices which is arisen with deconstructionism, reminds people must start from national practice, recognize the predominance and shortcoming of one's own national culture, neither narcissism nor self-humiliation.

本文在经济全球化的基础上，分析了经济交往过程中的文化全球化以及文化交往，阐释了文化交往的过程中，不同的文化由始初的相互冲突、对立、差异到相互理解、接受、直至达到认同的发展过程，论述了文化认同与文化趋同两种不同的文化现象之间的相互关系，叙述了在经济全球化、文化全球化时期人们的文化认同的心路，以及文化认同中的种种文化现象，详细地梳理了文化认同的历史演变，阐述了文化民族主义的文化认同、强势文化认同以及全盘西化的文化认同学说，比较客观地评价了西方文化在现代社会发展中的现实作用，同时也比较客观地分析了全球化时期由于解构主义兴起而出现的几个关于文化认同的其他声音，提醒人们要从本国实际出发，认清自己民族文化的历史优势与不足，既不要自恋，也不要自卑。

The logic way of thought of this thesis is culture, teacher culture and teacher culture of balkanization. This thesis starts with the definition of teacher culture, and teacher groups, and teacher culture of balkanization. Secondly, on the basis of definition and comparing domestic and overseas studies, this thesis analyzes the features of teacher culture of balkanization and its form of representation. Thirdly, this thesis explores the reason of the forming of teacher culture of balkanization from three angles of view, namely individual angle, organizational angle and social angle. Fourthly, this thesis analyzes the advantages and disadvantages of teacher culture of balkanization in a dialectic way. Fifthly, the thesis ends up with principles and strategies for modifying teacher culture of balkanization.

本文首先对教师文化、教师派别以及派别主义教师文化做了概念上的界定，在此基础上，参照中西方研究深入剖析了派别主义教师文化的特征，归纳了派别主义教师文化的具体表现形式；从个人层面、组织层面以及社会层面三个维度详实探讨了派别主义教师文化的形成原因；以辩证的观点全面分析了派别主义教师文化存在的合理性与弊端；在对合理性和弊端全面分析的基础上主要针对派别主义教师文化的弊端提出了矫正原则与策略；在对全文整体把握的高度上，本文认为应当采用审慎的变革态度和方式对派别主义教师文化进行恰当取舍，对于实质上是团体个人主义的派别主义文化，其解决的根本途径是走向合作。

Mainstream culture and none-mainstream culture, elate culture and mass culture, national culture and regional culture, Chinese culture and western culture composed for levels latterly and eight fields of content vertically, which requires us to classify the target audience according to their cultural background and provide more individualized communication strategy to serve for their core-value demand.

要求我们传媒针对目标受众群体文化背景进行选择分类与迎合，实施更为个性化的窄播策略，更为集中地服务于目标受众的中心价值。

Results The data of A were higher in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while lower in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration. The data of pH were lower in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while higer in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration.

结果 在0.5%、1%、2%浓度下，赤藓糖醇培养基的A值较木糖醇培养基高，pH值较木糖醇培养基低，说明变异链球菌在含0.5%、1%、2%赤藓糖醇的培养基内的生长和产酸能力明显较相同浓度木糖醇培养基高。

Chinese teacher culture develops from sacred culture,bureaucrat culture to public servant culture;meanwhile,western teacher culture develops from priestly culture,sacerdotal culture to expert culture.

圣贤文化、官僚文化和公仆文化依次是中国教师文化发展的历史轨迹，而西方教师文化的演变轨迹则依次表现为教仆文化、僧侣文化和专家文化。

By the comparison between archaeological stratigraphy, sediment characteristics of cultural layers and cultural interruptions, 46 ~(14)C chronological data of buried paleotrees and peat, the synthetical analysis of the distribution laws of sites, and the reference to the results from other scholars, we can see that there are 7 cultural interruptions in the three Gorges and 7 cultural interruptions in the Yangtze Delta. They are from the last stage of Chengbeixi Culture to the initial stage of Daxi Culture (7200～6000a BP)→the third stage of Guanmiaoshan Culture Style of Daxi Culture (5800～5500a BP)→the initial stage of Shangzhou Culture (4000～3500a BP )→the last stage of Shangzhou Culture (2500～2200a BP)→During the Song dynasty Culture (960-1276AD) During the Ming and Qing Culture (1368-1911AD) in the prehistorical and historical period in the three Gorges.

对长江三峡和长江三角洲考古遗址进行地层对比，考古遗址文化层和文化断层沉积特征、文化层的叠压关系、考古遗址淤砂和淤土层厚度、46处埋藏古树和39处泥炭的~(14)C年代统计数据和空间分布规律、文化遗址分布的时空变化规律等特征综合分析，发现长江三峡地区新石器时代到历史时期考古遗址地层中有7期文化断层：(1)城背溪文化末期到大溪文化初期(7200～6000a BP)；(2)大溪文化关庙山类型三期(5800～5500a BP)；(3)商周早期(4000～3500a BP)；(4)商周晚期(2500～2200a BP)；(5)宋代时期(960-1276AD)：(6)明清时期(1368-1911AD)；(7)近现代时期。

On the foundation to research achievements of home and abroad scholar about managing across-culture this text has analyzed our country across-culture corporation's present situation and problems, and finds out the problem source that effects the across-culture corporation ?culture divergence, and puts forward the method of handle culture divergence as well as culture mixing means of the across-culture corporation, then explores out concrete management approach of across-culture corporation.

本文在国内外学者对跨文化管理研究成果的基础上，剖析了我国跨文化企业现状及存在的问题，找出了影响跨文化企业发展的问题根源—文化差异，提出了处理文化差异的方法以及跨文化企业文化融合方式，继而探索出具体的跨文化企业管理实现途径。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。