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olatiles of different Chaenomeles sinensis cultivars in Shandong Province were investigated by means of HS-SPME coupled to GC/MS,and fruit aroma of different cultivars was studied.

中文摘要:用固相微萃取—气相色谱/质谱联用技术测定了山东省主栽光皮木瓜品种的挥发性物质,并对不同品种果实的香气成分进行了探讨。

Ethyl alcohol and-2,4-Decadienal were the primary volaties. Some unique and high relative content volatives, playing an important role in the formation of specific aroma of different Chaenomeles sinensis cultivars, were detected from most cultivars.

乙醇和-2,4-癸二烯醛等化合物是光皮木瓜果实内最主要的挥发性物质,绝大多数品种内还含有自己独特的且相对含量较高的挥发性物质,这对构成不同品种光皮木瓜特异香气起到重要作用。

The RAPD molecular marker can be used identify Chimonanthus praecox cultivars and study their relationships of these cultivars.

结果表明,RAPD标记可用于蜡梅品种鉴定和亲缘关系探讨。

To investigate Vitamin C and lycopene contents in different parts of watermelons fruit, 7 triploid seedless watermelon cultivars, including 6 red and 1 yellow flesh, were sampled to determine their total ascorbic acid, ascorbic acid, dehydroascorbic acid and lycopene contents. TAA, AA, DHAA and lycopene contents of 3 uncut red flesh seedless watermelon cultivars were measured after storage at room temperature for 2 months.

为了研究西瓜不同部位维生素C、番茄红素含量,对6个红瓤和1个黄瓤无籽西瓜品种果实不同部位总维生素C、还原型维生素C、氧化型维生素C和番茄红素含量进行测定,并检测红瓤无籽西瓜整瓜贮藏2个月后各成分损失率。

Besides, there still have never been reported about the situation of wild species originated from China in evolution of strawberry, research on cytogenetics of hybrids from the cross of wild species originated from China with cultivars of Fragaria will also be helpful for understand the relationship between wild species and cultivars and strawberry origin and evolution.

另一方面,原产我国草莓资源在草莓属进化中的作用至今尚未见报道,通过种间杂交及细胞遗传学研究可有助于探明草莓种质间的亲缘关系,为了解草莓的起源和进化提供参考。

Three donor wheat all show good character and had little genotypical difference in the aspect of inducement、differentiation、regeneration etc. They could all nice genotypical cultivars for wheat transformation. 3160 embryos have been used to transformation (960 embryos belongs to Atlas66, 1000 embryos belongs to EM12, 1200 embryos belongs to Scout66 respectively), At last, we got twenty-six of which were positive cultivars through PCR. The preliminary whole transformation frequency was 0.82 percent. Also the citrate synthase activities of the leaves of T_0 progeny show 5~9 higher than the control lines.

转化过程中分别使用了960个Atlas66、1000个EM12和1200个Scout 66幼胚(总记3160个),经抗性筛选、CS与标记基因的PCR检测初步表明有26株为阳性植株,总转化率为0.82%;同时进一步对T_0代植株叶片柠檬酸合酶以及T_1代植株经铝胁迫后对其根尖分泌物进行检测,结果在上述转化植株中检测到了柠檬酸合酶的活性,且转化材料比对照材料平均高出了5~9倍;同时铝胁迫后,T_1植株根尖检测到了苹果酸及柠檬酸的分泌。

The physiological and biochemical resistant mechanism of tea cultivars to tea brown blight caused by Guignardia camelliae. Making use of the mensuration of spectrophotometric analysis to study the enzyme activity of Peroxidas, Polyphenol Oxidas, Catalas, Phenylalamine Ammenia-lyase. The result showed that there was definite difference to the metabolic rule of defence enzymes activity in the annual leaf tissue of the resistant and susceptible cultivars after infecting.

茶树抗云纹叶枯病的生理生化机理研究利用分光光度计对茶树叶片中过氧化物酶、多酚氧化酶、过氧化氢酶,苯丙氨酸解氨酶的活性进行了测定分析,结果表明:感染茶云纹叶枯病菌前后,抗病品种和感病品种当年生成叶中防御酶活性变化规律存在一定的差异。

In this context the wild mango germplasm at Lapuo County, Guangxi, China were studied by using their biological characteristics and the amplified fragments length polymorphism molecular marker. These wild mangoes were found morphologically similar to Mangifera indica; and the AFLP markers showed that at the similarity coefficient of 0.63, 92 mangoes including cultivars, Mangifera sylvatica Roxb., wild mangoes, Mangifera. Siamensis Warbg. were divided into 14 groups and that the wild mangoes at Lapuo were close to M. sylvatica Roxb. and M. siamensis Warbg., but remained to fall in the middle of the cultivars and could not be separated from them.

结果表明:那坡县野生芒果在形态学上与普通芒果较为相似;AFLP标记显示,包括部分栽培品种、林生芒果、广西百色那坡县野生芒果、泰国芒果等在内的92份芒果种质在相似系数0.63的水平上可分为14组,广西百色那坡县野生芒果虽然与林生芒果、泰国野生芒果等芒果属其它种较为接近,但仍处于栽培芒果的中间,不能与栽培芒果分开。

These primer combinations produced a total of 1 119 bands, 229 specific loci (including 54 absent bands, and 175 monomorphic bands). Among them, 983 polymorphic bands, accounting for 88%, were detected. The percentage of identification per primer combination was as high as 100%. The average PPB of 14 foreign cultivars was 35.86% and the average PPB of seven domestic cultivars was 31.51%.

结果表明:8对引物共产生1 117条谱带, 229个特异位点(其中缺失带54条、单态带175条),多态带983条,多态带的比例为88%;每对引物鉴别效率为100%。14个国外银杏观赏品种平均多态带的比例35.86%,7个国内品种平均多态带的比例31.51%。21个观赏品种之间相似系数为0.4899~0.8499。

One could nodulate the soybean cultivars tested and showed wider host range, the other only nodulate limited soybean cultivars. Plasmid could not be detected from twenty-three slow-growing strains and twenty-nine fast-growing strains were found containing 1-4 plasmids by Echardts method.

质粒快速检测结果表明:慢生型大豆根瘤菌均不含有质粒,快生型大豆根瘤菌均有1-4个质粒,质粒类型与宿主亲和性也有一定的相关性。

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