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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

YEH S D%GONSALVES D Evalution of induced mutants of Papaya ringspot virus for control by cross protection Phytopathology , 1984,9

郑贵彬%符云华 TMV弱毒DN60-3等的诱变及其对番茄病毒病的保护效果陕西农业科学, 1986,6

Its pilots, Bertrand Piccard and André Borschberg, plan to cross the Atlantic in it and later to fly it around the world.

它的飞行员Bertrand Piccard和André Borschberg计划用它穿越大西洋,然后,环球飞行。

Ted Piccard of France and Daron Rahlves of the US crashed in mid-air at 80 km/h during the men's ski cross 8th final Sunday at the Vancouver 2010 Olympics in Canada.

法国选手特德·皮卡德和美国选手达伦·拉夫斯在21日温哥华冬奥会双板滑雪障碍争先赛1/8决赛中,以80公里的时速在空中相撞!

Other interesting exhibits include the Apostles' Stone, a fine 9th Century example of Pictish art , the Cross Slab from the 9th Century monastery, the Old Bell, removed during the 1975 restoration and marble statues of the 4th Duke of Atholl and Sir Donald Currie the 1908 restoration benefactor.

其他有趣的展品包括使徒石,罚款第九次皮克特艺术世纪的例子,从9世纪修道院十字板,老贝尔,在1975年恢复和杜克大学的阿瑟尔第四柯里和曾荫权先生的大理石雕像移走1908年恢复的恩人。

The character he shaped for "field" was a pictograph of ploughed field with furrows and cross-paths:.

于是象形文字的"田"便是一块有犁沟和田埂的耕地。

Such a picture box packed finished cross-stitch price is $800, embroidered semi-finished price is 160 yuan.

这样一幅装好框的成品十字绣售价是800元,没绣好的半成品售价则是160元。

In the hall uses the picture, the writing, the material object unifies, omni-directional application technologies and so on modern sound, light, electricity, unfold the idea annotation by the brand-new cloth four to cross the Chishui this Mao Zedong always most self-satisfied pen!

馆内采用图片、文字、实物相结合,全方位应用现代声、光、电等技术,以全新的布展理念诠释四渡赤水这一毛泽东平生最得意之笔!

The piercing principle of 2-roll cross-rolling piercer and its application range are discussed.

论述了二辊斜轧锥形辊穿孔机的穿孔原理及其应用范围。

It is pointed out that it is not a purpose for a rational technology of 2-roll cross-rolling piercer to try best to restrict cavities or eliminate them.

指出最大限度地抑制孔腔的发生,消除孔腔,不是二辊斜轧穿孔合理工艺追求的目标。

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推荐网络例句

Add it into your food such as to yogurt, cottage cheese and such.

你可以把它加入到像酸奶、奶酪一类的食物中一起进食。

The man was rescued about three hours later when his partner arrived home, SAPA said.

SAPA说,这为男子在三个小时后妻子回家后被解救

At that time, I am very happy!

那个时候,我很快乐!