查询词典 coverslip

They were cut into 3μm standby. Check logarithmic growth phase Saos-2 cells subcultured in Petri dish with the coverslip, which has been sterilized.

取Saos-2细胞系对数增长期细胞，传代于放置有消毒后盖玻片的培养皿中，培养24h制备细胞爬片，使用70%乙醇固定20分钟备用。

Septum bore of Transwell double-layer cell culture plate was 0.4 μm. In the co-culture group, osteoblasts were incubated in the upper chamber. In the control group, osteoblasts were not incubated in the upper chamber. Bone marrow mesenchymal stem cells were incubated on the pretreated coverslip in the lower chamber in both groups, for 20 days.

Transwell双层细胞培养板的隔膜孔径为0.4 μm，共培养组于培养上室接种成骨细胞，对照组培养上室未接种细胞，两组培养下室均于预处理的盖玻片上接种骨髓间充质干细胞，培养20 d。

Cervical mucus, semen consistency pilot test of time in the prediction of ovulation election period, a drop of glass on the first and fresh semen, cervical mucus and then take a drop of semen on the next, distance 2,3 mm, not to coverslip pressure to hand light light rock slide, so that two drops of liquid close to each other, under the microscope to observe sperm penetration capacity, such as the sperm can move through the mucus and to continue to run that sperm motility and cervical mucus of the characters are normal, mucus-free anti - sperm antibodies.

宫颈粘液、精液相合试验试验时间选在预测的排卵期，在玻璃片上先放一滴新鲜精液，然后取子宫颈粘液一滴放在精液的旁边，距离2、3mm，不要盖玻片加压，以手轻轻摇动玻片，使两滴液体互相接近，在显微镜下观察精子的穿透能力，如精子能穿过粘液并继续向前运行，表示精子活动力及宫颈粘液的性状都正常，粘液中无抗精子抗体。

Methods Macrophagea were cultured in 6-hole plate and coverslip was prepared. Then macropages were cultured one day with different concentrations(0, 12.5, 25.50, 100, 200 mg/L) of matrine, oxymatrine and sophordine; the expression of CD91 and CD13 by immunohistochemical dyeing was analyzed by Image-Pro plus system (Media Cybemetic, Inc., 6.0 edition).

(1)用6孔板培养巨噬细胞RAW264.7，制作细胞爬片，然后与不同浓度(0、12.5、25、50、100、200 mg/L)的苦参碱、氧化苦参碱和槐定碱共培养，细胞爬片免疫组化染色，检测CD91和CD13的表达情况，并采用Media Cybernetic、公司的图像分析系统进行定量分析，测量积分光密度值。

To establish the method of isolating the osteoclastic cells from the chick embryo, osteoclasts were isolated from long bones of the 18-day old chick embryo and the cultured on a glass coverslip or a bone slice.

从18日龄鸡胚长骨中机械分离破骨细胞於盖玻片和骨片上培养，以建立鸡胚破骨细胞体外培养方法。

The coverslip with differentiated cells were fixed at different timepoints and used for toludine blue or inmunohistochemical dying Meanwhile, different doses of antibody to Fibronectin were added to observe aggregating phenomenon.

用2，3传代细胞按一定比例种植于24孔板内，加入TGF-β1 1，5，10ng／mL，分别于不同时间点取材固定，用于甲苯胺兰组织学染色及抗纤维连接蛋白抗体免疫组化染色。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。