查询词典 coverslip

Fig.4 GFAP positive cells after plating clones onto poly-L-lysine coverslip 7 days in vitro(SABC-Cy3 immuno-fluocence).

图4 在多聚赖氨酸包被玻片上诱导分化7d后克隆细胞呈GFAP阳性(SABC-Cy3免疫荧光)。

The purified vWF was immobilized onto a coverslip. Cell rolling was induced in a parallel-plate flow chamber and observed by phase-contrast video microscope.

纯化的vWF固着在盖玻片上，在平行板液流室中进行细胞滚动研究，用相差电视显微镜观察。

When the cells have adhered to coverslip after being cultured for 24h, we use 70% ethanol to fix the cells for 20 minutes standby.

CD133表达流式细胞仪分析：分析对数生长期的骨肉瘤细胞系Saos-2细胞的CD133表达百分率。

Methods With cell floating method, which is separating the cultured cell from coverslip and putting the cell in front of perfusion tubing, whole cell patch clamp technique was used to record hASIC1a currents evoked by low pH external solution.

使用全细胞膜片钳技术，将记录细胞与玻片分离，并使细胞位于输液管前，呈漂浮状态，以记录在低pH值外液中，HEK293细胞的酸敏感性1a型离子通道的电流。

Fig.5 CNP positive cells after plating clones onto poly-L-lysine coverslip 7 days in vitro(SABC-Cy3 immuno-fluorescence).

图5 在多聚赖氨酸包被玻片上诱导分化7d后克隆细胞呈CNP阳性(SABC-Cy3免疫荧光)。

METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10^8/L were incubated in 12-well plate with a coverslip.

全骨髓法体外分离培养、纯化大鼠骨髓间充质干细胞，传至第4代时，按4×10^8 L^(-1)密度接种于置有盖玻片的12孔板内，制备细胞爬片。

Methods The neural stem cells derived from 12-12.5 day embryonic rat cortex were planted in suspended condition (three-dimension) and on coverslip precoated by poly-L-lysine.

取孕12～12.5天SD大鼠胚胎的大脑皮层细胞体外培养，建立神经干细胞模型。

METHODS: Co-culture without cell-to-cell contact: ZHJ-MAPCs and L02 hepatocytes were speared on coverslip separately, and then were put in a culture dish.

(1)间接共培养：将第4代ZHJ-MAPCs和人肝细胞系L02仅培养液相通行间接共培养。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

Controlled laminar flow through a rectangular tube was used to generate fluid shear stress (a range of 0-400dynes/cm〓) over a cell-lined coverslip or heart valve materials placed on the bottom of tube.

本文研制了内皮细胞切应力应力反应测试装置，对培养在Teflon表面的EC进行了切应力测试。这种装置可以调控切应力的大小。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。