查询词典 covalently

Otherwise, an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, the covalent binding of bioactive protein to the free terminal NH2 groups using glutaraldehyde as a coupling agent was followed.

并利用丙烯胺的电浆处理在钛金属表面键结上胺基(-NH2)的官能基，之后再以交链剂戊二醛来固定生物活性胶原蛋白於低温电浆处理后的钛金属表面。

Titanium plate first was cleaned by glow Discharge using argon plasma to eliminate surface contaminants and to produce a consistent and reproducible titanium oxide surface layer. Then an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, followed by the covalent binding of albumin to the free terminal NH2 groups using glutaraldehyde as a coupling agent. Surface morphologies were observed by scanning electron microscopy and atomic force microscopy.

样本以扫描式电子显微镜及原子力显微镜﹙AFM﹚观察表面形态变化，以能量分散光谱仪进行表面元素变化之定性分析，使用X光光电子能谱仪检测表面化学键结与元素变化作定性分析与半定量分析，并以胶样金免疫标示法配合使用场发射枪扫描式电子显微镜(Field Emission Scanning Electron Microscope， FEG SEM)，观察钛金属表面白蛋白连接情形作定性分析与定量分析。

The samples inhibited the binding of biotinylated lectins (from Canavalia ensiformis, Artocarpus integrifolia and Erythrina corallodendron, respectively) to microtitre plates with covalently bound carbohydrates (mannopyranoside, galactopyranoside and lactose, respectively).

将感染或未感染三代虫Gyrodactylus derjavini的虹鳟皮肤粘膜样品与甘露糖、半乳糖和乳糖反应以检测是否存在凝集素。

The tryptophan residues play important roles in DNA photoreactivation catalyzed by CPD photolyases. We have investigated the action mechanism concerning Trp in DNA photoreactivation, and obtained the results as follows: 1 We proposed a mechanism of photosensitized splitting of CPDs by Trp and Trp-containing peptides. 2 Quantum yields of splitting of the model compounds that a Trp linked covalently a CPD were obtained under 290 nm light in various solvents, these values changing with solvent, range from 0.01 to 0.5. 3 Utilizing pulse radiolysis technique, splitting processes were observed in aqueous solution of the CPDs with hydrated electron and some oxidative species (such as hydroxyl radical, bromic radical and azide radical), and obtained their rate constants. In the case of interaction between CPD and hydrated electron, splitting of the CPD anion, and pyrimidine anion transfer an electron to FAD or Rf, were observed. The process rate was slower one order more than that in photolyase. Minor, but highly mutagenic UV-light-induced lesions are (6-4) photoproducts, which are repaired by (6-4) photolyases. These enzymes, discovered in 1993, are not well characterized and their mechanism is still under investigation.

我们研究它的作用机理，获得以下结果：1）建立了色氨酸及其肽对嘧啶二聚体光敏化裂解的直接修复作用机理模型；2）获得了色氨酸共价键连接于嘧啶二聚体的化合物在多种溶剂中290nm光辐照裂解的量子效率，其值随溶剂而变化，范围在0.01-0.5；3）运用脉冲辐解观察到嘧啶二聚体与水合电子和一些氧化性的自由基作用裂解过程，得到相应速率常数；对二聚体与水合电子作用发生裂解以及向FAD返还电子的过程进行了体外模拟；4）（6-4）光解酶的作用机理中一个关键假定是氧杂环丁烷中间体在接受电子后能自发裂解，恢复二嘧啶的结构。

In this chapter, the folate-EDA was covalently connected with heparin- campotothecin by carbodiimide activation to get a folate-heparin-campotothecin conjugate.

以乙二胺作为连接臂，通过酰胺键将氨基化的叶酸与肝素-喜树碱复合物连接起来，制备了一种叶酸偶联的肝素-喜树碱复合物。

A new approach based on potential was firstly used to control DNA self-assembly covalently onto the SAM with the activation of 1-ethyl-3(3- dimethylaminopropyl)-carbodiimide and N-hydroxysulfosuccinimide.

研究了控制电位与DNA组装量的关系，并采用循环伏安、交流阻抗、俄歇电子能谱、原子力显微镜等手段对制得的电极进行了表征。

In order to mimic this repair process of apoenzyme, we synthesized two model compounds, in which tryptophan and/or tyrosine was covalently linked to cyclobutane thymine dimer, and measured the splitting quantum yield of dimer under 295 nm light. By investigating the photosensitized splitting properties of the dimer, some new insights into the intramolecular electron-transfer process were gained.

为了模拟酶蛋白的这一修复过程，合成了色氨酸和/或酪氨酸与胸腺嘧啶二聚体共价连接的化合物，作为酶-底物复合物的模型，研究了它们在295 nm光照射下氨基酸残基光敏化二聚体裂解的性质，测定了二聚体裂解量子产率，获得一些新的结果并对其进行了分析。

The fluorescence images that RhoCr1 coordinated with Cr~(3+)in PC12 cells showed RhoCr1 to be capable of penetrating into living cells and imaging intracellular Cr~(3+)changesFive probes have been designed and synthesized,where different aldehydes were covalently linked to rhodamine B dyes with diethylamine flexible chains.

吸收和荧光光谱的测试结果表明，探针RhoCr1在Tris-HCI中性缓冲溶液中能高选择性的识别Cr~(3+)并产生了荧光增强，通过对探针光谱性能的测试和TEPN滴定实验表明，探针RhoCr1与Cr~(3+)的络合是一个可逆的过程。

The plasmid DNA bands were detected clearly by agarose-EtBr gel electrophore sis.Two forms of plasmid DNA molecules,namely covalently closed circular DNA mol e-cules and open circular DNA molecules,were observed under electron microscope.

用琼脂糖-溴化乙锭凝胶电泳分析在两株菌的DNA中鉴别出明显的质粒带，用电镜方法在两株菌的DNA制剂中均观察到共价闭合超螺旋和开放型环状两种构型的DNA分子。

AAⅠcan bind covalently to the exocyclic amino group of purine nucleotides to form AAⅠ-DNA adduct. LC/MS/MS is a practical tool to detect AAⅠ-DNA adduct.

AAⅠ活化后能与腺嘌呤形成AAⅠ-DNA加合物，LC/MS/MS技术能够决速力便准确地检测AAⅠ-DNA加合物。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。