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In this study, cell counting was performed by using an inverted optical microscope and a hemocytometer.

三聚氰胺的IC50值通过由六条生长曲线回归得到的代时方程计算得到。

We examined cellular morphology under phase contrast microscope,cell proliferation using MTT assay,viable cell numbers by hemocytometer counting and cell apoptosis with flow cytometry.

当RA在高浓度(1μM、10μM、100μM)时产生明显抑制作用,细胞形态出现显著变化,48h较24h更明显。

Combined with the data of cell density by hemocytometer counting or MTT staining, the equations that account for the dependence of apoptotic/necrotic percentages on the fluorescence intensities of YP/PI were derived.

结合细胞密度的计数结果(细胞计数板、MTT比色法等),建立了用YP、PI荧光强度值计算细胞凋亡和坏死百分数的方法。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1,利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞,通过Western blot法检测转染细胞Bcl-XL蛋白的表达,流式细胞仪检测处理后细胞的凋亡情况,锥虫蓝染色法计数存活细胞。

Quiescent RASMCs were incubated respectively in 10% FBS DMEM as control group, 10% FBS DMEM supplemented with 0.1% dimethyl sulfide as vehicle group and 10% FBS DMEM supplemented with panaxynol(at concentration of 1,3,9×10-6 mol·L-1) as panaxynol groups. 1. Cell counting and double time. Cell viability was examined with trypan blue exclusion test and the cell number was counted at 0, 24, 48, 72h after the incubation using a hemocytometer.

实验中细胞分成如下几个处理组:①正常对照组:含10%FBS的培养液;②溶剂对照组:0.1%DMSO;③药物处理组:panaxynol 1、3、9×10-6 mol·L-1组。1、细胞计数及倍增时间测定:于接种并同步化后处理0、24、48、72h,用血细胞计数板计算活细胞数量,绘制生长曲线并计算细胞倍增时间。

Thus the Orks make remarkable progress by trial and error, without counting the cost, while other races steeped in high-flown philosophy simply fall into the same traps time and again, doomed to stagnate and decline.

就这样,绿皮们在不考虑代价的不断的尝试中有了进步。与此同时,其他种族迂腐夸张的世界观,人生观,价值观,让他们一次又一次的陷入绝境,他们注定走向停滞和倒退或者被蘑菇们征服。。。。

They're probably also counting on the prevalence of horse-race reporting, so that instead of the story being "McCain campaign lies," it becomes Obama on defensive in face of attacks.

"他们很可能也算对流行的赛马报告,使不是这个故事的"麦凯恩运动在于,"它会变成"奥巴马对防御在面对攻击。

Recent work by Susan Houseman concludes that US statistical data systems, which were set in place prior to the development of offshoring, are counting some foreign production as part of US productivity and GDP growth, thus overstating the actual performance of the US economy.

最近由苏珊豪斯曼通过研究总结出,美国的数据统计系统一直将海外生产发展作为优先考虑,一直将此部分生产作为美国生产能力和国民生产总值的一部分,因此夸大了美国经济的实际成就。

There are, indeed, gloomy and hypochondriac minds, inhabitants of diseased bodies, disgusted with the present, and despairing of the future; always counting that the worst will happen, because it may happen.

确实存在阴郁的和偏执的头脑,那些患病的躯体,厌恶当前,对未来绝望;因为也许坏事可能发生,总期待更坏的就要发生。

I am counting on young people with their idealism and energy to create a better future….

我期望着青年人以它们的理想和精力创造更美好的未来……)。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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