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The corneal thickness was measured with ultrasonic corneal pachymeter before enucleation and the corneal endothelial cell density was counted with non-contact specular microscope after enucleation in two groups.

两组均在眼球摘除前以角膜测厚仪测量角膜厚度，眼球摘除后以非接触角膜内皮显微镜行角膜内皮细胞计数。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

Cells in the bron choalveolar lavage fluid were counted and differentiated, as well as the amounts of total protein, albumin and the activities of alkaline phos phatase, lactate dehydrogenase and type Ⅳ collagenase (matrix metallco proteinases, MMP-2, MMP-9) were detected.

计数支气管肺泡灌洗液中的细胞数并行分类，测定总蛋白、白蛋白含量及碱性磷酸酶、乳酸脱氢酶及Ⅳ型胶原酶(MMP-2、MMP-9)活性。

Additionally one kind of novel that enrols discharge is phyletic, novel of blame military affairs not was counted.

另外一种招流量的小说种类，非军事小说莫数了。

Then we counted the money and found Tracey collected 50 precent more.

在点了钱数后，我们发现特蕾西筹集的钱款比米歇尔多了 50%。

Methods: A brain concussion was produced in rats using a simple pendulum device model. One control (n=12) and six experimental groups were used(n=12 in each group). The cerebral concussion groups were assigned to sacrifice times ranging from 1 day to 24days after injury.A Morris Water Maze memory paradigm was used to assess learning and memory function. Pycnosis degeneration or necrosis neurons in the locus cerebral cortex, dorsal hippocampus, dentate fornix and brainstem reticular formation were observed and counted after brain concussion. Cholinergic neurons in the basal forebrain and brainstem reticular formation were identified and quantitated using choline acetyltransferase.

应用自制单摆式闭合性机械打击装置建立脑震荡大鼠模型；成年SD大鼠随机分为1个对照组(n=12)和6个脑震荡组(即伤后1、2、4、8、16、24d，n=12)；应用Morris水迷宫(Morris Water Maze，MWM)实验评价大鼠的学习记忆功能；用光镜对大鼠的上述脑区的固缩变性坏死细胞进行观察和计数；用免疫组化方法检测基底前脑和脑干网状结构的胆碱乙酰转移酶(cholin acetyltransferase，ChAT)阳性细胞的表达。

In 2001 he was asked to participate in the management of www.treddi.com a small italian CG portal ,that at the time counted up a bit more than 1000 users ;at the present it is a point of refence for all italians fascinated by computer graphics ,by January2008 it had over 30,000 registered users,In 2001,he also became aware of a small plug-in,VRay,developed by chaosgeoup ,a Bulgarian software-house .since then he has not stooped being interested in everything that has to do with the world of rendering systems ,especially VRay .

In 2001年，他被要求参与管理 www.treddi.com 一个小意大利协商小组的门户，这在当时清点了超过1000位用户；在目前它是一个点refence所有意大利迷上了计算机图形学，由January2008它已超过30000个注册用户，在2001年，他也开始意识到，一个小插件， VRay ，制定chaosgeoup ，保加利亚软件内部。从那以后他一直没有弯下腰感兴趣一切，是同世界的渲染系统，特别是VRay 。

After the animals were killed, retrogradely labeled surviving RGCs were counted in the flat-mounted retinas and the mean RGC density was estimated.

于相应存活时间点处死动物，取出各组大鼠左侧视网膜平铺后计数存活RGC并得出RGC的平均密度。

And "baptism of desire," which is received by those who, implicitly or explicitly, desire baptism but are prevented from receiving it sacramentally. Even those who through no fault of their own do not know Christ and his church may be counted as anonymous Christians if their striving to lead a good life is in fact a response to his grace, which is given in sufficient measure to all.

，和&洗礼的愿望，&这是所收到的那些谁，暗示或明确，欲望的洗礼，但均无法接受sacramentally &即使那些谁没有犯错，自己不知道基督和他的教会，可能被视为无名氏的基督徒，如果他们的努力导致一个良好的生活其实是在回应他宽限期，这是由于在足够的措施，向所有&。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新