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Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

After cultured for 4 days,the cells were stained with TRAP. Multinucleated(≥ 3 nuclei) TRAP-positive cells were counted as osteoclasts, and the expression of TRAP mRNA were determined by RT-PCR.

2.0？10.0？50.0 ？滋g/ml，对获得的各组破骨细胞行抗酒石酸酸性磷酸酶染色计数阳性破骨细胞(核≥ 3)的数目；提取总RNA用逆转录-聚合酶链反应检测TRAP mRNA的表达？

Naoto Matsukura has made his intentions clear here in Thailand by topping the time sheets when it counted most, as qualifying for the 1:12 IFMAR World Championships got underway at the RCS in Bangkok.

直人松了他的意图显然在泰国的时间超过表计时，最，因为排位赛的1：12 IFMAR世界田径锦标赛上获得正在进行的RCS在曼谷举行。

METHODS:The content of the total naphthoquinone was counted in L-shikonin ,methanol was used as solvent and the wavelength was set at516nm.

总萘醌的含量以左旋紫草素计，溶剂为甲醇，检测波长为516nm。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组，以体外直肠不全结扎法造模，以动态浊度法检测各组大鼠血浆内毒素水平，放射免疫分析法检测各组血清TNF-α含量；②大鼠处死后，取左肺进行支气管肺泡灌洗，以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平；③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数，计数兰染的细胞数，求出死亡百分率；在光镜下计数BALF沉渣200个细胞，计算中性粒细胞所占百分比。

If we counted all the people who visited the exhibition last week,we will get a surprise numble

我们如果把过去一周的展览会参观人数加起来，我们会得出一个惊人的总数。

Methods After ZTO injection acted on human ovarian cancer SKOV3 cells, the changes in the nucleus were observed by fluorescence microscope, oncosis index was counted by projection electron microscope, the situation of cell DNA breakage was observed by agarose gel electrophoresis, and cell Bcl-2 gene expression was observed by immunohistochemical method.

以不同浓度莪术油注射液作用人卵巢癌SKOV3细胞后，通过荧光显微镜观察细胞核变化、透射电镜计算细胞胀亡指数、琼脂糖凝胶电泳观察细胞DNA断裂情况、免疫组化观察细胞Bcl-2基因的表达。

In the programs of prevention and care of ophthalmopathy,the programs whose provide ratio is high than 80% counted for 81.82 percent of the total 11 programs.

在眼病防治中，提供率达到80%以上的项目占所有眼病防治项目的82%。

Mosquitoes captured and the egg positive traps were counted and recorded every day. Results Comparing with the control group, yeast-generated CO2 could take a strong attraction to mosquitoes in the field (P.05), but had an adverse effect in laboratory. P. maximum infusion, tyre infusion and L-lactic acid dilution could not attract Aedes albopictus to oviposit in Mosq-ovitrap. The colour change of Mosq-ovitrap could not raise its attractant ability to mosquitoes, on the contrary, increase the probability of mosquito escaping from Mosq-ovitrap.

结果 实验室和现场的结果显示，酵母发酵产生的CO2在室内实验中没有增强诱蚊诱卵器的诱蚊效果，而在现场实验中却产生较好的诱蚊作用，实验组的诱蚊指数和诱蚊密度指数与对照组比较差异均有统计学意义(P.05)；大黍叶、轮胎浸出液和乳酸稀释液在试验中未显示出吸引白纹伊蚊雌蚊产卵的能力；在改变诱蚊诱卵器杯身颜色的试验中，杯身上2/3变为黑色并不能提高它的诱蚊和诱卵能力，反而增加成蚊逃逸的概率。

The corneal thickness was measured with ultrasonic corneal pachymeter before enucleation and the corneal endothelial cell density was counted with non-contact specular microscope after enucleation in two groups.

两组均在眼球摘除前以角膜测厚仪测量角膜厚度，眼球摘除后以非接触角膜内皮显微镜行角膜内皮细胞计数。

Summary In copyright infringement cases,if the alleged infringer uses or even plagiarizes the copyrighted works or parts thereof,which causes no substantially adverse effect on the normal exploitation of the work,nor does it inflict any substantial damages to the right owner,the de minis doctrine should be applied to exempt the liabilities of the defendant and seek the balance of interests between the right owner and the general public embodied by the copyright law.

在侵犯著作权案件中，如果被控侵权行为人虽未经著作权人的同意，使用甚至抄袭了受著作权保护的作品或作品的片段，但若情节轻微，未对该作品的正常使用产生任何实质不利的影响，亦未对权利人的权利造成实质性的损害，则应适用&不计琐细原则&，免除行为人的责任，以寻求在权利人利益与著作权法所体现的社会公共利益之间的平衡。

This new product will become our best seller.

这个新产品会成为我们最畅销的产品。