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Father said not to mind him, he said that he could just as well stay home , and that he was sure that he could put in the time woking in the garden; he said that there was a lot of rough dirty work that he could do, like digging a trench for the garbage, that would save hiring a man, and so he said that he'd stay home; he said that we were not to let the fact of his not having had a real holiday for three years stand in our way; he wanted us to go right ahead and be happy and have a big day, and not to mind him.he said that he could plug away all day, and in fact he said he'd been a fool to think there'd be any holiday for him.

父亲叫我们不必管他，他说他留在家里也不错，而且他相信他能利用这段时间在花园里干点活；他说那里有一大堆他可以干的粗活和脏活，比如挖个垃圾坑什么的，这就免得雇人来干了，所以他愿意留在家里；他说我们也用不着顾虑他三年来一直没有过过一个真正的假日这回事；他要我们马上出发，快快活活地过这个节，不要为他操心。他说他能够整天埋头干活，而且，真的，他还说，本来，他有过个什么节这种想法就是想入非非。

Conclutions: Under ischemic condition, the decrease of Iks could prolong the mid-late repolarization phase of APD. It could cause EAD and then arouse TdP. Because the decrease of M cell was significantly greater than that of sub-endocardial myocytes and sub-epicardial myocytes, it could increase TDR. Cardiac arrhythmias could last due to reentrance that secondary the increase of TDR.

缺血时，心肌细胞的Iks电流减弱，使得细胞动作电位复极中后期延长，容易出现EAD并引发TdP；由于M细胞的Iks电流减弱程度明显大于心内、外膜细胞，造成心室跨壁复极离散度增加，有利于折返的发生，使得心律失常得以持续。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

In the future, I could manage a kindergarten, a training company, a toggery or a jewelry store. I want to marry to a reasonable gentleman. I wish I could have a daughter who would be a pianist in the future. If I have a son, I wish he could be a CEO of a company. In my old age, I would like to translate some poems to count my thumbs. These ideas could be realized in any sciential family.

现在做教师，将来可以自己开幼儿园、培训学校、服装店、珠宝店，嫁一个讲道理、能持家的人，一定要生一个女儿，长大了让她当钢琴家，如果是男孩就让他自己开公司。

And yet I could only sense, could only see, could only imagine. There was nothing but silence from the very unexceptional human girl. I could hear nothing.

可是，我也就只能感觉，只能看，只能猜测，这个极其普通的人类女孩脑子里什么声音都没有，我什么都听不到。

When the gas flux is constant, the ratio of reduced resistance is improved with the increase of the water stream velocity. However, the relationship between the gas flux and the ratio of reduced resistance is more complex. When the water stream velocity is constant, if the gas flux is small in contrast, the effectiveness of reduced resistance is not obvious. But when the gas flux increased, the jetted gas will form """"archform"""" which will augment the shape resistance. In this condition, resistance could not be reduced and could even be increased, if the gas flux continues to be increased. So that there needs setting a baffle called step, which introduces the jetted gas into the turbulence boundary layer. In this case, the shape resistance may be fairly great. Besides, the waterline of model has also some effect on the ratio of reduced resistance, which acts on the size of wave. If waterline is deep, the wave could not affect the deployment of microbubble layer, so there is almost no effect on ratio of reduced resistance. However, the wave would affect distributing of microbubble layer under the model so that the gas could leak out from the two sides of model and directly affect the ratio of reduce resistance if the waterline is very small. Whether installing the dashplate or not has also the great influence on the effectiveness the ratio of reduced resistance. From the results of experiment, conclusion is that adding dashplate will greatly improve the effectiveness of reduced resistance.

当喷气量一定时，水流速度增大，减阻率提高；喷气量与减阻率之间的关系比较复杂，速度一定时，如果喷气量较小，减阻效果就比较差，但当喷气量增大的话，喷射出的气体就会形成一个&拱度&，增加了船模的形状阻力，在这种情况下最好采取断阶喷气，把气体引入到湍流边界层中，在这种情况下，船模的形状阻力比较大；船模的吃水对减阻率也有一定的影响，主要表现在波浪大小上，如果吃水较深，波浪影响不到微气泡层的分布，对减阻率不会造成太大影响，如果吃水较浅，波浪就会影响到微气泡层在船模底部的覆盖情况，可能会有部分微气泡从船模两侧泄漏，这样就直接影响到了减阻效果；是否安装压浪板，对减阻率也有较大的影响，从试验结果可以看出，在船模尾部加设了压浪板时，减阻效果明显得到改善。

The results is two strains of bacteria Pseudomonas alcaligenes (No. 1203) and Cellulomonas (No. 1120) that could endure 60 times of the V water quality standard were selected, that is, 2 strains could grow in 6mg Pb /L and 60mg Cu /L media. Another 2 strains could endure 70 times of the standard were attained, Rhodococcus (No. 1314) and Acinetobacter (No. 1410), which could grow in 7mg Pb /L and 70mg Cu /L media.

共筛选出耐国家水质V类污水重金属Pb和Cu 60倍（Pb 6mg/L，Cu 60mg/L）的菌株共2株，1203号菌（产碱假单胞菌Pseudomonas alcaligenes）和1120号菌（纤维单胞菌Cellulomonas）；耐V类重金属Pb和Cu 70倍（Pb 7mg/L，Cu 70mg/L）的菌株2株，1314号菌（红球菌属Rhodococcus），1410号菌（不动杆菌Acinetobacter）。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

结果：酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间，给药剂量为80μg／kg／d时疗效最显著，达到69.24％，在20μg／kg／d-80μg／kg／d剂量范围内生命延长率为50-70％，给药剂量与荷瘤鼠生存时间呈现一定量效关系；酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长，给药剂量为160μg／kg／d时疗效最显著，抑制率为44.03％，并且在40-320μg／kg／d剂量范围内抑制率为25-50％，给药剂量与肿瘤抑制率呈现一定量效关系；酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用，在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显，其中浓度为100μg／ml时抑制率达19.36％；酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤：体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与效应细胞对照组相比有显著性差异(P＜0.05)杀伤率分别达到35.58％、61.2％；体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P 。05)，杀伤率分别达到21.39%、47.63%；裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P.05)，最高杀伤率分别达到32.86%、73.07%；酪丝亮肤能增强单核巨噬细胞系统的吞噬功能，吞噬指数与生理盐水组比较有显著性差异(P.05)；酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与效应细胞对照组相比有显著性差异(P.05)；酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与生理盐水对照组相比有显著性差异(P.05)；酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO，IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰，酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能，而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。

The result indicated that, although microorganism from paddy soil could directly reduce Cr, the reduecing rate and efficiency were limited; the higher Cr concentration was, the less reducing ability the microbial showed; the microbe from different paddy soils had different effects on Cr reducing abilities; The reducing ability of the microbe from Sichuan paddy soil was higher than that from Jiangxi paddy soil. Cr added could be rapidly reduced in paddy soil under anaerobic incubation, moreover, the process of dissimilatory iron reduction of soil could obviously promote the Cr reduction. The existent of Cr could delay the appearance of Fe; the higher Cr concentration was, the later Fe appeared. The delayed time of Fe appearance was in accordance with the ending time of Cr reduction.

结果表明，由水稻土中提取的微生物虽然能够直接还原Cr，但其还原速率和还原程度有限，且Cr浓度越高，微生物的还原能力越差；不同来源的上壤微生物对Cr的还原能力有所差异，其中四川水稻土中微生物对Cr的还原能力均大于江西水稻土；在厌氧培养的水稻土中，添加的Cr可以较迅速地还原，其中土壤氧化铁的微生物还原过程对Cr还原具有明显的促进作用；Cr的存在导致Fe生成的时间出现滞后，目Cr浓度越大生成Fe的滞后时间越长；Fe产生滞后的时间与Cr还原结束的时间具有一致性。

Positive rate was relatively high, and the foreign gene could also maintain in testicular tissue for a long time, it could still be detected in the tissue in 7 months after being injected. However. Deferent results could be gained when the detection were made with different primers on different sites of the recombinant plasmid. This could be explained by transgene lost when the foreign gene recombinated and integrated in heterogenetic cell, or transgene degradated by cell nucleases.

然而，我们也看到，在后代检测中，利用不同的引物扩增重组质粒不同部位时，检测结果存在差异，转基因阳性率不同，这种差异说明当外源基因进入到异源细胞内，在重组整合时发生了外源基因丢失的现象，并由此导致外源基因的整合率下降，也可能是受细胞内核酸酶的作用，使得外源基因遭到降解。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。