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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Neither cleric nor conjurer could fathom what could be the cause of this change in the lands.

牧师和魔法师都弄不清什么导致了这片土地的变化。

In Crystal Palace it was a different game. I was feeling they could be a defensive side with everybody closed behind the ball on a small pitch. I felt three players in the midfield could be enough to control it and some more width up front would be better for us.

"对水晶宫的比赛非常的艰难，我觉得他们会全线退守，所以我安排了3个中场这足以控制比赛，而前场多一个人会更加有机会，一切都非常好！"

Josie : You could be a clown,And I could be one of your references.

你可以当小丑，而我可以当你的推荐人之一。

Who but a cockeyed optimist could possibly imagine that relationships between the currencies of widely diverse national economies could remain rigidly fixed over prolonged periods, if not indefinitely?

除了昏头昏脑的乐观主义者，还有谁能想象，差别很大的不同国家经济体，它们的货币之间的关系能够长期固定不变呢？

The Colburn heat transfer factors Jh correlation could describe 96.6% of experimental data and the Colburn mass transfer factors Jm correlation could describe 81.4% of experimental data with an error of ±15%.

开发了反映空气侧传热特性的Colburn传热因子Jh和反映传质特性的Colburn传质因子Jm的关联式，所开发关联式在±15%误差范围内分别能涵盖96.6%和81.4%的实验数据，其平均误差分别为5.3%和8.5%。

The gaseous impurities in the alloys were well controlled with the sum of C, N and O less than 500wppm. For the first time it was found the strong interaction between Cr-Ti-CON, which reduces the mobility of Ti and CON, enhancing high-temperature strength and the aging-hardening property, and keeping the cold-work induced hardening at higher temperature. Aging-hardening could increase the tensile strength of V-4Cr-4Ti by 30% and could be utilized at 00 C for better economical performane of a vanadium structure. Hydrogen release will occur for the alloys tensile loaded at room temperature, which will affect the safety of a vanadium structure due to the resulting dimensional instability. For hydrogen embrittlement, impact toughness and fracture toughness of the alloy showed higher sensitivity than the tensile test elongation,so the toughness should be used as a new standard for the assessment of the embrittlement. Vanadium alloys were easily oxidized in air.

合金的气体杂质含量得到有效控制，C、N、O总量低于500wppm；首次发现Cr-Ti-CON之间的强相互作用，它使Ti、CON的高温可移动性降低，提高合金的高温强度、增强时效强化特性、使冷加工强化保持到更高温度；时效强化可使V-4Cr-4Ti的强度提高30%，可在低温（00C）下应用，提高构件的经济性；室温下，钒合金在拉应力的作用下，存在着氢释放，带来部件使用中的尺寸不稳定性，影响构件安全；氢脆是钒合金聚变应用的一个关键问题，合金的冲击韧性和断裂韧性表现出比拉伸延伸率更强的氢脆敏感性，应作为评价合金氢脆行为的新标准。

Pharmacological experiments indicated that low concentration of GABA could promote the growth of radicle and coleoptile and high concentration of GABA could restrained their growth, Presumedly the inhibitory effect had something to do with increase of endogenous ethylene level.

胞质中积累大量GABA与谷氨酸脱羧酶定位于胞质中有关，细胞壁中GABA则是与GABA的运输和胞间分配有关，核中存在大量的GABA的功能尚不清楚。

A calibration model was established by introducing a planar target that could move freely with three collinear and known mutual location points. The calibration point coordinates in a camera coordinate system could be calculated by using the imaging information of three points and the light stripes on image plane of the camera.

引入一个可自由移动的平面靶标，靶标上只需要共线且相互位置确定的3个特征点，利用共线三点建立三点透视数学模型，根据3个特征点以及光条纹在摄像机像面的成像信息，获取了光平面上标定点在摄像机坐标系下的坐标。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。