查询词典 cotyledonary

Tumefaciens mediated transformation using cotyledonary node as explants was optimized; 3 methods including cutting chapiter, ovary injection and chapiter spreading for pollen tube pathway was compared. Cutting chapiter and ovary injection can both achieve insect resistant gene tranformants.

优化了以子叶节为外植体的农杆菌介导转化技术；比较了切割柱头、子房注射和子房涂抹3种花粉管通道法处理授粉后黄瓜子房的转化效果，切割柱头和子房注射均可成功获得黄瓜抗虫转基因植株。

A diarch or triarchsiphonostele with exarch primary xylem was found at lower cotyledonary node zone.

果表明：银杏幼苗的轴向器官以子叶节区下部的初生维管结构先建立，其中柱类型是外始式二原型或三原型管状中柱

The three day old seedlings grown in medium supplemented with 5 mg/L 6-BA were used to prepare the cotyledonary-node explants. The cotyledonary-node explants had one cotyledon and half of the embryonic axis.

选用在高达5 mg/L 6-苄氨基嘌呤(6-BA)的种子萌发培养基上生长3 d的蒺藜苜蓿幼苗，制备获得由1片子叶和半个胚轴组成的子叶节外植体。

The maturation process of somatic embryos could be divided into four stages: ESM. globular and torpedo embryoid, pre-cotyledonary embryoid, mature cotyledonary embryoid.

体细胞胚的成熟过程大致可分四个时期：胚性胚柄团、球形胚至鱼雷形胚、子叶前期胚和子叶期成熟胚。

The results of changes of endogenous hormone contents at early stage of floral differentiation in cotyledonary nodes of cucumber seedlings after decapitation were as follows: The contents of IAA and ZT in cotyledonary nodes of the cucumber seedlings withi...

ZT/IAA、GA3/IAA比值的变化趋势相似，0至2天上升，2至3天下降，3至4天再上升。根据实验结果，联系黄瓜去顶苗成花的形态变化过程，对激素质量分数变化与花芽分化之间的关系进行了讨论。

There were 2 peaks of starch accumulation in the process of somatic embryogenesis,the first time was at the stage of embryogenic callus,the second time was at the stage of globular embryoids;and a small raise appeared at the stage of torpedo-shaped embryoids,and the content of starch was slightly higher than those of heart-shaped embryoids and cotyledonary embryoids.With the development of embryos,the tendency of the changes of the content of DNA,RNA and the total nucleic acids was approximately similar,and the peak values were all at the stage of cotyledonary embryoids,and the change of the content of RNA was more remarkable than those of DNA and the total nucleic acids.The tendency of change of the content of proteins almost took on the S-shaped curve.

结果表明：龙眼胚性愈伤组织体胚发生过程中淀粉有2个累积峰值，第1次出现在胚性愈伤组织阶段，第2次出现在球形胚阶段，在鱼雷形胚阶段又有一个小回升，这时淀粉积累较心形胚和子叶形胚阶段高一些；随着胚胎的发育，DNA、RNA、总核酸含量的变化趋势大致相同，最高点均出现在心形胚阶段，其中RNA含量的变化较DNA、总核酸含量明显，蛋白质含量的变化曲线呈&S&形。

The results of changes of endogenous hormone contents at early stage of floral differentiation in cotyledonary nodes of cucumber seedlings after decapitation were as follows: The contents of IAA and ZT in cotyledonary nodes of the cucumber seedlings within 0~4 d after decapitation decreases while the level of GA3 first increases then declines within 1~3 d, after that it increases again.

对花芽分化初期子叶节中内源激素质量分数的测定表明，IAA质量分数在花芽分化初期处於较低水准，ZT质量分数呈下降趋势，GA3质量分数则出现升-降-升的波动状态。

Agrobacterium tumefaciens-mediated transformation system of Medicago truncatula cotyledonary-node explants Abstract The three-day-old seedlings of Medicago truncatula, which geminated in medium containing up to 5mg/L 6-BA, were used to prepare the cotyledonary-node explants which contained one cotyledon and 1-2mm of split hypocotyl. High-efficiency regeneration system of Medicago truncarula was developed by axillary shoot organogenesis of cotyledonary-node explants.

论文第二部分 Medicago truncatula遗传转化和植株再生体系的研究第一章根癌农杆菌介导的Medicago truncatula子叶节外植体转化系统的研究本文利用在高达5mg／L 6-BA的萌发培养基上生长3天的Medicago truncatula幼苗，制备获得由一片子叶和1-2mm切分的胚轴组成的子叶节外植体。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Longan somatic embryogenesis occurred through the following stages—embryogenic callus, globular embryoid, heart-shaped embryoid, torpedo-shaped embryoid, cotyledonary embryoid and matured cotyledonary embryoid, which was similar to that of zygotic embryo.

龙眼胚性愈伤组织的体细胞胚胎发生与合子胚的发育过程基本相同，依次经过愈伤组织、球形胚、心形胚、鱼雷胚、子叶胚以及成熟胚等阶段。

Do you know, i need you to come back

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。