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The factors relevant to the running buffer and their effects on separation were investigated. The buffer solution composed of 30 mmol/L sodium borate and 2.4 mmol/L β-CD was found to be the most suitable electrolyte for separation. The second order dissociation constant range of honokiol was estimated. The content range of honokiol in Cortex Magnoliae Officinalis and was 3.42-5.98 mg/g, while the mean content of honokiol in its preparation 0.021 mg/mL.

优化了厚朴提取物的分离条件，考察了缓冲溶液浓度、pH 值、β-CD的添加浓度对分离的影响，确定了最佳的分离缓冲溶液组成为30 mmol/L 硼砂和2.4 mmol/L β-CD，pH 值为9.48；推测出和厚朴酚的二级离解常数pKa2 的范围为9.94-10.83；在优化的分离条件下，测定了厚朴药材和藿香正气口服液中和厚朴酚的含量分别为3.42-5.98 mg/g、0.021 mg/mL，和厚朴酚的线性范围是0.020-0.405 mg/mL，其线性相关系数R=0.998(n=5)，加样回收率为98.4-103.5%，RSD 为3.2-5.7%(n=3)。

To study the protective effect of N-acetylcysteine on the injury in mitochondria of cerebral cortex in rats caused by sodium cyanide.

目的］研究N-乙酰－L-半胱氨酸(N-acetylcysteine， NAC)对氰化钠(sodium cyanide， NaCN)所致大鼠大脑皮质线粒体损伤的保护作用。

As one kind of important drugs of ardrenal cortex hormone, dexamethasone sodium phosphate is widely used in clinical application.

地塞米松磷酸钠是一种重要的肾上腺糖皮质激素类药物，在临床上有十分广泛的应用。

There were some changes in the expression of sodium pump α-subunit gene in the cortex of kidney of the 1k1c hypertensive rat, which might be related to the development of hypertension in this hypertensive model.

1k1c高血压大鼠肾皮质钠泵α亚单位基因表达发生改变，这种改变可能参与该模型的血压升高机制。

Weeks later, all the rats were killed, the levels of sodium pump α1-,α2-, and α3-subunit mRNA and protein in the cortex of kidney were detected with reverse transcription polymerase chain reaction method and immunohistochemical assay, respectively.

分别应用RT-PCR及免疫组化技术，探讨1k1c高血压大鼠肾脏皮质钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。

RESULTS: The mRNA levels of sodium pump α1-subunit increased and α2- and α3-subunit were unchanged in the cortex of kidneys from 1k1c hypertensive rats compared with control rats, while no change has been found for all the three subunits gene expressions at protein level.

结果：正常大鼠肾脏皮质主要表达钠泵α1亚单位，α2与α3亚单位表达较弱；在mRNA水平，1k1c高血压大鼠肾脏皮质钠泵α1亚单位表达明显较强，α2与α3亚单位表达无改变，而在蛋白水平各亚单位基因表达与对照组比较均无明显差异。

Thus, salicylate is widely used for studying the mechanisms of tinnitus. The aim of the present study was to investigate the effects of salicylate on voltage-gated sodium, potassium and calcium channels in the inferior colliculus and the auditory cortex neurons by use of the whole-cell patch clamp method, in order to obtain insight into the relations of tinnitus and voltage-gated channels.

本研究首次将膜片钳的实验技术和离子通道的基础理论引入耳鸣中枢机制的研究中，利用全细胞膜片钳记录技术，观察水杨酸钠对大鼠听觉通路上的两个重要部位——下丘、颞皮层的神经元细胞膜上电压门控性钠、钾、钙离子通道特性的影响，探讨听觉通路上神经元的电压门控性离子通道与耳鸣的关系。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达，由原始生殖细胞产生卵原细胞，髓质退化成为卵巢腔，分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加，它们在分化结束后解体，分化前细胞内有较多的大颗粒，在分化中大颗粒减少，小颗粒增多，在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜，髓质有许多睾丸管构成，管腔内有散落排列的精原细胞。

The content of cortex spermidine and spermine decreased during 6h and 12h ischemia.

在相应时间，脑组织病理改变呈相应变化。

To detect effects on apoptosis of cortex neuron in DHCA rats, the rat model of occluding bilateral common carotid artery in the deep hypothermic (21C) rats had been established, and flow cytometry, TUNEL, electron microscope, immunohistochemistry and RT-PCR had been studied.: part I: To establish a new rat model of cerebral ischemia/protect during DHCA: 20 SD rats were used in the experiment, little animal respirator was to assist anesthetized rat's respiration after tracheal cannula, artery press through artery spile in femoral artery, electrocardiograph, temperature, blood saturation of O2 (SaO2) and arterial blood gases were monitored during the whole experiment, blood flow of bilateral common carotid artery of rat were occluded about 60 minute when its temperature was reduced to 21 C by ice, then normal temperature was resumed.

本研究采用大鼠深低温颈总动脉阻断模型，应用流式细胞仪、电镜、原位缺口末端标记法、免疫组化法和RT-PCR技术分别检测深低温脑缺血对皮层神经细胞调亡的影响方法：第一部分深低温停循环脑保护大鼠模型的建立：SD大鼠20只，麻醉后气管插管辅助呼吸，用冰块将大鼠的体温降至(21±1)℃，阻断双侧颈总动脉，记录体温、心率、血压、血氧饱和度和血气分析，60分钟后恢复颈总动脉血流，用Longa等的5级评分标准评定4小时和24小时后大鼠神经系统损伤情况。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。