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The DNA sequences corresponding to the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand were cloned to the transcription plasmid pGEM3Zf.

以1a型负链3'末端RNA为模板，NS5B1和NS5B2为聚合酶，进行体外的RNA合成反应，斑点杂交和RT-PCR检测产物，确定纯化的蛋白具有RdRp活性。

The tumor-bearing mice received corresponding treatments for 15 consecutive days, after which their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices. The tumor mass was also weighed and the tumor inhibition rate calculated.

连续用药15d。第15天处死小鼠，计算胸腺指数、脾指数；剥离瘤块称重并计算抑瘤率；用免疫组织化学方法检测各组荷瘤小鼠肿瘤组织的增殖细胞核抗原的表达强度。

It is important to develop new k-space trajectories and corresponding image reconstruction algorithms for higher imaging speed.

本文的主要工作是为了缩短国内在这方面与国外的差距，研究比较了基于螺旋轨迹采样数据的网格化重建算法，同时给出了一个基于PC机的磁共振图像重建软件。

FPM were classified into 3 degrees and corresponding treatments were offered on the basis of (1)The biomechanics mechanism of FPM,(2)The transposing characterization of FPM,(3)The degree that fractures injuried articular surface,(4)If surrounding tissues were injuried and the stable state of mortise.

依据(1)后踝骨折的生物力学机制；(2)后踝骨折移位的特点；(3)骨折波及关节面的损伤程度；(4)是否合并周围组织损伤及踝穴的稳定状况，将后踝骨折分为Ⅲ度，得出了与之相应的治疗方案。

At first, the typical 2D and 3D triangulation algorithm of scattered points is discussed in this paper. Then, a space partition strategy with its corresponding data structure and encoding method is proposed by the author in order to improve the triangulating and searching efficiency according to the trend of larger and larger points set.

本文在详细研究和分析了典型平面及空间散乱数据三角剖分算法思想后，根据散乱数据点规模越来越大的趋势，提出了一种大规模散乱数据的空间划分方法及相应的数据结构和编码方案，缩小了在剖分时搜寻和遍历数据点的空间范围，提高了算法效率。

The results corresponding hasbeen drawn a lot,however,studies on FRP Tubed reinforced concrete columns have been verylimited(The FRP tubes are used to transversly confine concrete,not bearing the axialloads).Based on experimental and theoretical analysis,the mechanical properties of the GFRPtubed reinforced concrete columns under axially loaded are researched.

而针对内置钢筋的FRF套管混凝土结构的研究较少(套管指FRP管不承受轴压力，仅起约束作用)，本文通过试验和理论分析，着重研究了GFRP套管钢筋混凝土组合柱的轴压力学性能。

Taylor, professor of psychology at Tufts and corresponding author of the study.

泰勒的心理学教授塔夫茨和相应的研究报告的编写者。

Based on the above investigation of ideal layer-by-layer growth process, the surface roughening phenomena during the real epitaxial growth are discussed. Some important microscopic dynamic models for molecular-beam epitaxy , which have attracted much attention recently, are studied. Using the master-equation method, the corresponding growth equations are directly derived, and then the scaling behaviors, the universalities, as well as the crossover effects are determined.

在这一理想层状外延生长研究的基础上对实际外延生长过程中十分显著的表面粗化现象进行了探讨，深入研究了几类近年来受到广泛关注的描述分子束外延生长的重要的微观动力学生长模型，采用主方程方法直接得到了相应的非线性生长方程，并确定了标度性质、普适类以及渡越行为。

With the development of society, the science and technology raises gradually, the use of automobile more and more be universalized, and the corresponding industries such as the motor beauty shop are also growing in number or quantity increasingly at the same time, and the scope is enlarged gradually, the before one motor beauty needs of shop is very hard satisfied in all vocational work distance traveled by a stream of water and document management work of handwork statistics of tradition.

随着社会的高速发展，科学技术的迅速提高，汽车的使用越来越普及，同时汽车美容店等相应的产业也日益增多，规模逐渐增大，传统的手工操作业务流程和资料管理已经很难满足当前汽车美容店的业务需求。

With the development of society, the science and technology raises gradually, the use of automobile more and more be universalized, and the corresponding industries such as the motor beauty shop are also growing in number or quantity increasingly at the same time, and the scope is enlarged gradually, the before one motor beauty needs of shop is very hard satisfied in all vocational work distance traveled by a stream of water and document management work of handwork statistics of tradition.

设计的指导思想是：一切为客户着想，界面美观大方，操作简单明了，而且作为一个实用的管理系统要有良好的容错性，在用户出现误操作时能尽量及时地给出警告，以便用户及时改正。计算机资源是受到限制的，因此要充分利用Visual Basic 6.0和Access 2000数据库的功能，设计出功能强大软件的同时，尽可能地减少对系统资源的占用。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。