查询词典 corneal rupture

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Corneal allograft is the most effective treatment for corneal blindness,but corneal graft rejective reaction is the main cause of corneal allograft. Corneal neovascularization is one of the most causes of corneal blindness and the high-risk factor of corneal graft rejective reaction.

研究背景同种异体角膜移植是治疗角膜盲最为有效的方法，而角膜移植排斥反应仍然是移植失败的主要原因；角膜新生血管是角膜盲的主要原因，亦是角膜移植排斥反应的高危因素。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明，处理后4周，全周角膜缘上皮板层手术切除，中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化，未发生睑球粘连，角膜基质胶原纤维完整未见溃疡、穿孔等病变，细胞印迹学检查为结膜表型，可作为实验性角膜缘干细胞移植的病理模型；全周角膜缘上皮板层手术切除，中央角膜上皮用生理盐水擦除的5只试验家兔，有2只为结膜表型，另3只为角膜表型，观察期内结果不稳定；半周角膜缘上皮板层手术切除，中央角膜上皮层用生理盐水擦除的5只试验家兔，角膜表面透明，全部为角膜表型；直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔，有4只角膜基质胶原纤维断裂、溶解，并伴有严重的溃疡、穿孔、睑球粘连等病变，不能用于移植试验，另1只角膜表面透明，未见结膜和新生血管长入，细胞印迹学检查为角膜表型。

Thirty-two pathological corneal samples were collected from the patients during corneal transplantation, including keratoconus (3 cases), endothelial keratitis (1 case), corneal endothelial decompensation after cataract operation (4 cases), Mooren's ulcer (3 cases), corneal implant opacities (5 cases), herpes simplex viral leucoma (1 case) and perforation (3 cases), trachoma corneal leucoma (1 case), bacterial (1 case) and fungal (1 case) corneal perforation, chemical trauma (5 cases), and burn (2 cases).

32例角膜植片标本来自我院角膜移植取下的病变组织，其中园锥角膜3例；角膜内皮炎1例；白内障术后角膜内皮失代偿4例；蚕食性角膜溃疡3例；角膜植片混浊5例；单疱病毒性白斑1例和单疱病毒性角膜穿孔3例；砂眼角膜白斑1例；细菌性和真菌性角膜穿孔各1例；角膜化学伤5例和热烧伤2例。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

This paper reviews whether forward movement of the posterior corneal surface occurred, changes in posterior corneal power and curvature, changes in posterior corneal astigmatism and tilt, changes in posterior corneal asphericity and BFS after the surgery.

本文综述了准分子激光屈光性手术后角膜后表面是否发生前移改变、角膜后表面屈光力、曲率、散光度、轴度、非球面特性以及最适球面等是否发生改变的研究进展。

Eye examination showed blepharospasm ball conjunctival hyperemia, edema, corneal punctate epithelial shedding, decreased corneal sensitivity, corneal fluorescein staining showed diffuse points, or flake coloring, the pupil to narrow as .24 hours, the symptoms and reduce the growth of new epithelium, 48 hour recovery, when the electro-optic stimulation of ophthalmia after symptom onset, can be partially points, 025% dicaine pain, point anti-inflammatory eye drops, hourly, pre-B anti-corneal infection .

眼部检查可见眼睑痉挛，球结膜充血、水肿、角膜上皮点状脱落，角膜知觉减退，角膜荧光素染色呈弥漫性点状或片状着色，瞳孔缩小。24小时内症状随着新生上皮的生长而减轻，48小时可痊愈，当电光性眼炎的刺激症状出现后，可局部点025％地卡因止疼，点消炎眼药水，每小时一次，预B防角膜感染。

According to the corneal thickness measured, a 7.0mm Hessburg-Barron corneal trephine was used to incise cornea into different deep defect in respective experimental group and placed 7.75mm SIS implant over the corneal defect.The implant was continuous sutured over the defect.The reaction of white rabbit corneal was observed and recorded everyday within postoperative observation period constituted 105 days.

根据所测量的家兔角膜厚度，使用直径7mm的HessBurg-Barron负压环钻分别对三组实验家兔的角膜植床取不同的深度，植入直径为7.75mm猪小肠黏膜下层，并将其连续缝合于植床上。

Corneal epithelial implantation is 5 in 2000(0.25%). The rate of diffuse intralamellar keratitis is 5.5%(110/2000); corneal opacity are 2 in 2000(0.1%). Group B: Free cap are 2 in 500(0.4%); Corneal epithelial implantation is 1 in 500(0.2%);Diffuse intralamellar ketatitis are 27 in 500(5.4%), not any incomplete cap,broken flap and corneal opacity happened.

结果 A组：不完全瓣21眼占1.05%（21/2000）；游离瓣13眼占0.65%（13/2000）；碎瓣1眼占0.04%（1/2000）；角膜上皮植入占0.25%（5/2000）；弥漫性板层角膜炎占5.5%（110/2000）；角膜混浊2眼占0.1%（2/2000）。B组：游离瓣占0.4%（2/500）；角膜上皮植入占0.2%（1/500）；弥漫性板层角膜炎占5.4%（27/500），无不完全瓣、碎瓣及角膜混浊发生。

It is possible that houses were outrageously priced one year ago and that they are still priced much higher than the national average.

情况有可能是，这里的房价曾贵得离谱，现在的房价仍然远高于全国的平均指数。

This is a mother's love for her child.

这是母亲对孩子的关爱。