查询词典 cornea

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

His academic career includes appointments as an assistant in ophthalmology at harvard medical school, clinical instructor at cornell university medical college, assistant professor of ophthalmology at the state university of new york at brooklyn, and attending staff surgeon at the cornea service at manhattan eye, ear and throat hospital.

他的学术事业包括担任哈佛大学眼科系的助理、康奈尔大学医学院诊所顾问、纽约州立大学布鲁克林分校眼科学的助教、曼哈顿眼耳喉医院眼角膜科担任外科医生以及纽约州立大学布鲁克林分校实习医生培训主任。

The distribution of the cornea with many sensory nerve fibers in the ability of the ciliary, ciliary nerve is to make the face and head of the first half of the produce touch, temperature and pain perception of a large branch of the trigeminal nerve.

角膜的许多在纤毛，纤毛神经感觉神经纤维的能力分配是使脸部和对生产触摸，温度和痛苦的一大分支三叉神经感觉上半年头。

Ocular tumors in dogs are found in the eyelid, conjunctiva, third eyelid, cornea, sclera, iris, ciliary body, retina, choroid, optic nerve, and orbit.

犬类的眼部肿瘤发现于眼睑，结膜，第三眼睑，角膜，巩膜，虹膜，睫状体，视网膜，脉络膜，视神经和眼眶。

The cornea is also distributed in a relatively small number of voluntary movement can stimulate the nerve fibers in the lacrimal gland.

角膜是还分布在自愿的运动数目较少，可以刺激泪腺的神经纤维。

Vacuum freeze-drying experiments and methods on keeping active cornea of animal and human being were researched.

通过对动物和人类眼角膜进行冻干保存实验，对冻干保存方法进行了系统的研究。

In human beings, infection may cause inflammation of the cornea and external ear.

人类若感染上这种病菌就会导致角膜和外耳发炎

This paper try to summarize the analysis of the crystalline lens change, the effect of the cornea; the biomechanical effects of age and presbyopia during the process of eye accommodation.

作者对人眼在调节过程中有关晶体的改变、角膜的影响，以及年龄和老花眼有关调节的生物力学研究进行综述介绍。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。