查询词典 cooling effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

In some conventional fan revolution speed control methods, variable control of the number of revolutions of a cooling fan is performed by a process comprising steps of detecting an actual temperature of cooling target fluid, ie fluid to be cooled by the cooling fan, such as hydraulic oil and circulating radiator cooling water for cooling an engine (such cooling water is hereinafter referred to as coolant), determining a target fan revolution speed by a system of a proportional integral control unit based on the difference between the actual temperature detected and a target temperature, and changing the fan revolution speed based on the target fan revolution speed so as to bring the actual temperature of the cooling target fluid down to the target temperature.

在一些传统风扇转速控制方法，控制变量的数目革命的一个冷却风扇，是由一个过程组成的步骤检测到一个实际温度冷却目标流体，即流体被冷却，由冷却风扇，如液压石油和散热器循环冷却水用于冷却发动机（如冷却水是以下简称为制冷剂），确定一个目标风扇转速由一个系统的比例积分控制单元基础上的差异，实际温度检测和目标温度，并改变风扇转速的基础上，针对风扇转速，使实际温度的冷却液指标下降到目标温度。

This paper hereby introduces the cooling process of the common 160,000 m^3-in-capacity atmospheric LNG storage tank in China, the preconditions and matters to which attention is paid upon the cooling of tank. It also discusses some commissioning techniques needed in the cooling process, including pressure control, cooling medium supply and its flow control, process monitoring and cooling rate control, etc. The paper points out that the displacement of pipelines is easily to be overlarge and flange leakage takes place frequently in the cooling process and it gives corresponding solutions.

为此，详细介绍了国内LNG接收站常用16×10^4 立方分尺常压LNG储罐的冷却过程，分析了LNG储罐冷却前应具备的条件及注意事项，讨论了LNG储罐冷却过程中的压力控制、冷却介质供应及流量控制、冷却过程的监控及冷却速率控制等调试技术，指出了冷却过程容易出现管线位移过大、法兰泄漏等问题，并给出了相应解决方法。

A cigarette production apparatus comprising a cooling water system for circulating a cooling medium along a perfume supply path connecting an application nozzle for applying a perfume to a roll paper with a pump for perfume supply to the application nozzle to thereby cool the perfume, wherein the temperature of cooling medium fed to the cooling water system is controlled by means of a cooling unit.

一种香烟制造装置，其沿着连接有在卷纸上涂敷香料的涂敷嘴和向该涂敷嘴供给香料的泵的香料供给路，设置有使制冷剂循环从而使上述香料冷却的冷却水系统，利用冷却单元来控制向该冷却水系统供给的上述制冷剂的温度。

This thesis developed physics model of radiant panel heating and cooling room based on previous studies. Computational fluid dynamics software FLUENT was applied to study the thermal environment of radiant panel heating and cooling room. The distributions of temperature and velocity of rooms of radiant floor heating and cooling, radiant ceiling heatingand cooling and radiant wall heating and cooling are gained.

本文在总结前人经验的基础上，分别建立了辐射板采暖和辐射板供冷房间的物理模型，利用商业软件Fluent对采用辐射板采暖系统和辐射板供冷系统房间内的热环境进行了模拟研究，分别得到了辐射板布置在地板、墙面和顶板三种不同方式下的采暖和供冷房间内的温度和速度分布情况。

The results showed that clay mineral of the sedimentary core B106 reveal seven palaeoclimate events since 16725 Cal.aBP, such as"Bolling Warming Events"in 13100±45 aBP,"Younger Dryas Cooling Events"in 10393±45 aBP,"Early Holocene Cold Event"in 8000aBP,"South of China Cooling Events"in 4624±35 aBP, Cooling Events in 3370±40 aBP, Drought Incident Cooling Events in 922BC and"Cooling Events of Northern and Southern Dynasty"in 514AD.

研究结果显示，B106柱的粘土矿物学指标指示出16725 Cal.aBP以来的7次古气候变化事件，分别是13100±45 aBP的&博林暖期升温事件&、10393±45 aBP的&新仙女木降温事件&、8000 Cal.aBP对应的&全新世最大降温事件&、4624±35 aBP的&中国南方降温事件&、3370±40 aBP的降温事件、922BC的干旱降温事件和514 AD的&南北朝降温事件&。

The results indicate that the cooling rate of melt droplet that can not break further depends not only on its diameter, but also on its position in atomizing chamber. As a result, the same sized particles in different positions show different cooling rates and two different kinds of microstructures. The cooling rates of melt droplets range from 104 to 108 K/s, and the critical cooling rate for glass formation of Al-Ni-Y is about 105 K/s. Moreover, the corresponding maximum diameter of amorphous particles is 25 μm, which accords well to the theoretical analysis results. The new approach, reflecting the complexity of close-coupled gas atomization objectively, is available and effective to evaluate the cooling behaviors of close-coupled gas atomization.

结果表明：在一定的工艺条件下，紧耦合气雾化存在多种破碎模式，熔滴的冷却速率因直径、雾化位置的差异，相同直径熔滴经历结晶和非晶化2种不同冷却行为；依据Al基合金粉末的粒度分布和微观结构，结合熔体的雾化过程及冷却模式，确定熔滴冷却速率的上、下限；实验得出熔滴的冷却速率为104~108 K/s，Al-Ni-Y合金的非晶化临界冷却速率为105 K/s，能形成非晶态的熔滴最大直径为25 μm左右，实验结果与理论分析结果较吻合，比较客观地反映紧耦合气雾化的复杂过程。

This study experimentally examined the heat transfer characteristics of 180-deg turning duct under forced convection cooling, as the internal cooling structure of 180-deg turning duct is widely applied to industrial cooling. While several perforated holes were separately arranged within the 180-deg turning duct, the cold flow of better cooling capability was guided in the first duct into the second duct in advance, so as to reduce the overheating of the second duct and realize uniform cooling of the machine members. In addition, the centrally separated holes could yield extra turbulence against the air current, and the interaction between bypass air current and mainstream may possibly improve the overall heat transfer capability.

本研究系以实验方法探讨180度转弯通道於强制对流冷却下之热传递特性，180度转弯通道之内部冷却构型在工业冷却之应用非常广泛，本研究设计将180度转弯通道其中间分隔具多个穿孔，目的在将第一流道内具较佳冷却能力之冷流提早引入第二流道，以减少第二流道产生过热情况，如此可使得机件获得均匀之冷却，此外，中间分隔上之穿孔对气流也具有额外之扰动效果、流经穿孔之旁通气流与主流交互作用，这都有可能增益整体热传能力。

Given the same total flow effusion from holes, the results show that the heat shield gets a higher average cooling effectiveness when the opening ratio is 3% compared with 6%; cooling effectiveness is sensitive to the height of ripple board, the heat shield gets the highest cooling effectiveness when the dimensionless height of ripple board is 1% compared with 2% and 3.33%; but there is a little influence on the cooling effectiveness of the liner with the change of the diameter of holes and the height of cooling passage.

研究表明：在气膜孔出流总量相同的情况下，3%开孔率比6%开孔率的隔热屏平均冷却效率较高；波纹板高度对隔热屏冷却效果影响较大，波纹板无量纲高度为1%比波纹板无量纲高度为2%和3.33%的隔热屏平均冷却效率高；孔径和冷却通道高度的改变对隔热屏冷却效果几乎没有影响。

The main cooling system of the wind driven generator provided by the invention comprises a hydraulic pressure station heat exchanger, an electric generator heat exchanger, a gearbox heat exchanger, a gearbox temperature controlling valve, a generator temperature controlling valve, a main cooler and a cooling pump, wherein, the dimensional length of the cooling plate of the main cooler is 1 to 2 percent larger than the length of the original cooling plate, the thickness is 2 to 3 percent larger than that of the original cooling plate, and the width is not changed.

本发明的风力发电机主冷却系统，包括液压站热交换器、发电机热交换器、齿轮箱热交换器、齿轮箱温控阀、发电机温控阀、主冷却器和冷却泵，其中，所述主冷却器的散热板尺寸长度比原来散热板的长度大1％-2％，厚度比原来散热板的厚度大2％-3％，宽度不变。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48