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Transgenic plants expressing GNA showed significant insecticidal activity towards \\omoptera insects such as aphids and brown planthopper in bioassay and feeding tests.In this paper a lectin gene was firstly isolated from leaves of Zephyranthes grandiflora, belonging to Amaryllidaceae, by the use of rapid amplification of cDNA ends technique. Characterization, functional prediction and construction of plant and prokaryotic expression vector of this novel gene were studied.

本实验采用cDNA末端快速扩增(Rapid amplification of eDNA ends，RACE)技术，首次从中国特有的野生植物韭莲(又名风雨花，Zephyranthes grandiflora)的叶片中分离克隆出了一个新的凝集素基因，对基因进行了序列分析和功能预测，并成功地构建了韭莲凝集素基因的植物表达载体和原核表达载体。

In this paper, the cDNA sequence from Ampullaria crossean stomach named Dy1300 was obtained by RT-PCR firstly and cloned into vector pMD 18-T. Its ORF named SXYN contains 882bp nucleotide encoding a 294 amino-acid protein. The percentage of similarity of SXYN with the reported cellulase EGX gene sequence from Ampullaria crossean was 89.8%.

本研究首先通过RT-PCR的方法从福寿螺胃中扩增出新的纤维素酶cDNA序列―Dy1300，并与克隆载体pMD 18-T相连接，通过对其全序列的分析，确定其开放阅读框ORF长度为882bp，命名为SXYN(GenBank， AY941794)，推定的氨基酸序列含294个氨基酸，与已报道的福寿螺纤维素酶C端基因的同源性达89.8%。

Malaria is a vector-borne communicable disease transmitted by Anopheline mosquito.

疟疾是由疟蚊传播的疾病，被受感染的疟蚊叮咬后，潜伏期通常为12至30日。

To explore the feasibility of the mitochondrial DNA as transgenic vector, we translated the mtDNAs of the Antheraea pernyi, Diaphania pyloalis Walker and the fish to the silkworm. Through microscopic examination and mtDNA polymorphism analysis, we tested the maintenance of the foreign mtDNA in the silkworm and investigated the influence of the foreign mtDNA to the physiological behavior.

为了探索线粒体DNA作家蚕转基因载体的可行性，本实验将柞蚕、桑螟和鱼的线粒体DNA转入家蚕体内，通过着色镜检、线粒体DNA多态性分析检测了外源线粒体在家蚕细胞中的存留情况，并调查了外源线粒体对家蚕生长的影响。

AIM: To realize an antiricin neutralizing monoclonal antibodybased ScFv proteins with high biological activity in the pCDNA3.1 vector expression system and to evaluate the neutralizing activities of ScFv.

目的：实现基于中和性单抗4C13单链抗体的真核表达，并对其生物学活性进行评价。

METHODS: The total RNA was obtained by TRIzol from an antiricin neutralizing monoclonal antibody hybridoma cell named 4C13 and heavychain and lightchain variable fragments were amplified by overlap PCR and accepted by Genbank of NCBI. VH and VL were linked by3 with a direction of VHlinkerVL and cloned to pCDNA3.1 vector expression system.

用TRIzol提取抗ricin中和性单抗杂交瘤细胞4C13总RNA，扩增其轻、重链可变区基因，登陆GenBank；用Linker3将VH和VL连接，用重叠延伸PCR克隆入真核表达载体pCDNA 3.1，用脂质体转染293T细胞，对瞬时表达产物进行生物学活性检测。

Cotton aphid, Aphis gossypii (GlovcrXHemiptera:Aphididae) is one of the most important pests to various plants,and also an efficient vector of several plant viruses.

棉蚜属同翅目蚜科蚜属，世界各地均有分布，属古北、东洋、澳洲、非洲、新北、新热带6大区系共有种。

System computes combined navigation system of GPS/IMU, VECTOR and ascendable geometry stable platform, proceeds in space registration and time synchronization, calculates the systems accurate space, time, stance and course. Planar laser scanning data was filtered, registered space-wisely and time synchronously and fuse computered with other sensors data, extracted to 3D solid model of characteristic information of the road and outside of street, continuously extracted to point, line and plane, reconstructed combined with CCD camera data and visualized to 3D model true scene, finally implement acquisition and process of 3D space data timely, initiatively and integrally.

该套系统通过对GPS/IMU组合系统导航解算、VECTOR和升降几何平台进行空间配准、时间同步处理，计算出系统精确可靠的空间时间和姿态航向信息，二维激光扫描仪数据经过滤波去噪、和其他传感器数据融合、三维空间计算，解算出道路和两侧街景的三维空间数据模型，对空间激光数据模型提取点线面特征值，结合CCD相机数据进行立体重建和可视化，实现了实时、主动、完整地获取和处理三维空间数据信息。

System computes combined navigation system of GPS/IMU, VECTOR and ascendable geometry stable platform, proceeds in space registration and time synchronization, calculates the system??s accurate space, time, stance and course. Planar laser scanning data was filtered, registered space-wisely and time synchronously and fuse computered with other sensor??s data, extracted to 3D solid model of characteristic information of the road and outside of street, continuously extracted to point, line and plane, reconstructed combined with CCD camera data and visualized to 3D model true scene, finally implement acquisition and process of 3D space data timely, initiatively and integrally.

该套系统通过对GPS/IMU组合系统导航解算、VECTOR和升降几何平台进行空间配准、时间同步处理，计算出系统精确可靠的空间时间和姿态航向信息，二维激光扫描仪数据经过滤波去噪、和其他传感器数据融合、三维空间计算，解算出道路和两侧街景的三维空间数据模型，对空间激光数据模型提取点线面特征值，结合CCD相机数据进行立体重建和可视化，实现了实时、主动、完整地获取和处理三维空间数据信息。

The 1 kb scaffold-attachment region at 5' non-transcription region of rRNA gene of silkworm Attacus ricini was cloned into eukaryotic expression vector pLu, which contained luciferase report gene and neo selecting marker.

蓖麻蚕核糖体RNA基因非转录间隔区有一长约1kb的核骨架结合区(scaffold-associated region，SAR)。

Liapunov—Schmidt method is one of the most important method in the bifurcation theory.

Liapunov—Schmidt方法是分叉理论的最重要方法之一。

Be courteous -- even when people are most discourteous to you .

要有礼貌──即使当別人对你最不礼貌的时候。