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The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸橙1-氨基环丙烷-1-羟酸氧化酶基因cDNA片段,将片段序列在NCBI网站上进行同源性搜索,显示的皆为不同植物的ACC氧化酶基因,因而认为所克隆的片段就是酸橙ACC氧化酶基因;并运用DNAStar5.0软件进行序列分析,推导的氨基酸序列为278个残基;具有所有植物ACC氧化酶基因共有的保守区域;与多种植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上。

Some new concepts and algorithms are proposed,such as jobnomial sequence,job perturbation sequence,neighbourhood of job nomial sequence and jobsequence perturbation analysis.These job sequence PA algorithms extend PA techniques aboutcontinuous parameters to discrete parameters and provide some new kinds of tools for solving suchcombination optimization problem.

论文提出了工件序的邻域、摄动序等概念以及对工件序进行摄动分析的思想和算法,将传统的PA算法从连续参数领域推广到离散参数的组合优化领域,为此类组合优化问题增添了新的求解工具。

First 46 viral sequence's PA fragments in year 2009 are analyzed. Then the siRNA target genes with strong ability of interference are selected on the basis of the secondary structure of siRNA target sequence. Our research reveals that the outbreaking H1N1 virus in 2009 is characterized by steady heredity, high sequence conservativeness, uniform siRNA target gene sequence, and high conservative structure.

对2009年的46株病毒序列的PA片段进行分析,从经过序列分析所获得的众多靶系列中,采用结构分析手段对靶序列进行筛选,获得较易干扰的靶序列及设计出相应的siRNA。

Basing on theregional sequence stratal framework, carry through the elaboratecompartmentalization of sequence stratum in major horizon and basing on seismicand logging data, use seismic sequence stratigraphy and drilling sequence stratigraphyto discuss isochronous stratal distributed pattern in framework.

在建立区域层序地层格架的基础之上,对重点层位进行精细层序地层划分,在地震资料和测井资料的基础上,利用地震层序地层和钻井层序地层,探讨等时地层格架内的地层分布模式。

Highprecision sequence stratigraphy was applied to divide and correlate the different sequence stratigraphical units from the Upper Cretaceous to plaeogene in the Biyang depression, and a sequence stratigraphic framework which can correlate the different sequence stratigraphical units was set up to point out that the ramp region of the northdepression is an oil and gas reservoir featured by altitude progradational delta and complex fault blocks, the inner belt of the north slope hinge zone is a low order faultlithologic oil and gas reservoir, the development stages and high order terms of the deep depressed area is a fluxoturbidite and sublacustrine fan lens trap, and the south actic region is a lithologic hydrocarbon reservoir development area.

运用高精度层序地层学的理论,系统地划分和对比泌阳凹陷上白垩统至古近系不同级别的层序地层单元,建立了凹陷内各级层序地层单元的对比框架,指出凹陷北部斜坡带为高位进积三角洲复杂断块群油气藏,北部斜坡枢纽带内带为低位扇断层-岩性油气藏、深凹区湖扩展期和高位期为滑塌浊积体及湖底扇透镜体圈闭,南部陡坡带为岩性油气藏发育区。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术,分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因,以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,分别自43例、51例慢性HBV感染患者血清中扩增出目的片段,构建质粒Teasy-BCP和Teasy-pre S2,酶切鉴定后,采用聚丙烯酰胺凝胶电泳技术展示缺失突变,再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

The nucleotides sequence and deduced amino acid sequence of these cDNA figments mentioned above were divided into two fractions. One is carbon part of L gene , the other is 5 terminal,and they were compared with other rabies-related virus strains published in NCBI previously.The result show that the homology of nucleotide sequence and amino acid sequence of carbon part in L gene among these two rabies virus strains is 91.3% and 95.8%, and these two strains belong to type of gene I.

本实验以吉林鹿源DRV8202株及河南鼠源野毒株MRV的L基因的C端为主要研究内容,用RT-PCR和3'-RACE方法对其进行了克隆和测序,将所测得的核苷酸序列和推导的氨基酸序列分成两个部分,一是L基因C端序列,另一个是L基因5'端的非编码区,并与NCBI上公开发表的狂犬病毒其它毒株的相应片段进行分析比较。

The research efforts of seismites and seismic deposition, earthquake-tsunami sequence, vibrational liquefaction sequence in carbonate rock, seismites and seismic unconformity, sabkha seismites sequence, the autochthonous seismic liquefaction sequence in clastic rock were summarized.

对中国在海相和陆相地层的震积岩与震积作用、地震-海啸序列、碳酸盐岩振动液化地震序列、震积岩与震积不整合序列、萨布哈震积岩序列以及中生代陆相地层中碎屑岩原地系统的地震液化序列等方面的研究成果进行了综述。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明,6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异,表明HIV1的LTR及其下游基因置于痘病毒启动子控制下,在痘苗病毒中表达时有下述特点:(1)不同的痘苗病毒启动子与全长LTR相互作用,对gag基因表达有显著不同的调控效果;(2)NR序列对Gag蛋白表达没有明显影响;(3)EN序列不能被重组痘苗病毒表达系统识别;(4)TAR序列可提高Gag蛋白的表达量;(5)U5区及下游非翻译序列不影响Gag蛋白的表达。

Sequence analysis revealed that nucleotides sequence of 28 kD protein gene varied among different isolates of wheat yellow mosaic virus.28 kD protein genes of the Luotian and Huangchuan isolates comprised 762 nucleotidesand were less 3 nt than others,located at the site of 326,327 and 336 nt,respectively.The different isolates shared homologies ranging from 92.1% to 96.9% in nucleotide sequence and 89.8% to 97.3% in amino acid sequence.

序列分析结果表明,小麦黄花叶病毒不同分离物的28kD蛋白基因的核苷酸序列存在一定的差异:湖北罗田和河南潢川分离物在第326,327和336位较四川雅安、江苏扬州及日本分离物缺少3个核苷酸,前两者核苷酸长度为762nt,后三者为765nt;不同分离物核苷酸序列同源性从92 1%到96 9%,相应的氨基酸序列同源性从89 8%到97 3%。

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