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Most recently, a transgenic silkworm system was used to produce a fusion protein containing a collagenous sequence.

最近，一种转基因蠕虫吐丝系统开始生产含有胶原构序的融和蛋白。

And, we selected some representative species of Collembola and representative groups of Protura, Diplura, Insecta and Arustacean to sequence and analyze their 18S rRNA completely gene sequences, 28S rRNA D6-D7 domain and nearly completely gene sequences to make the studies about the phylogeny relationships of the main families and orders of Collembola, discuss the questions such as the reliability about the new four orders system to Collembola, the monophyletic and ordinal phylogenetic relationships of them and the situation of the special groups, the phylogenetic relationships about Collembola with other groups and the evolution of Hexapod.

同时选取弹尾纲的代表种类和原尾纲、双尾纲、昆虫纲、甲壳动物等节肢动物部分类群，进行了18S rRNA基因全序列和28S rRNA基因D6-D7区以及近全长序列的测定、分析，对弹尾纲重要科属的亲缘关系、进化地位进行分子系统学研究，讨论弹尾纲最新分类系统中四目的确定性、各目单系性、四目的系统关系、特殊类群的分类地位，以及弹尾纲和其它类群之间亲缘关系、六足动物进化等问题。

Hox genes are expressed in a collinear fashion and temporo -spatial sequence.

Hox基因在表达上具有强烈的时空性和共线性。

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

Methods The yield of combinant human IL-18 expression in E.coli remains quite low because the sequence of mature hIL-18 has 37aa rare condons for E.coli in a total of 157aa.

它们的分子中没有N-糖基化位点，不存在二硫键，很可能巯基就是分子的活性中心。

Methods The yield of combinant human IL-18 expression in E.coli remains quite low because the sequence of mature hIL-18 has 37aa rare condons for E.coli in a total of 157aa.

设计特异性引物，以RT-PCR从外周血单个核细胞中扩增出人白细胞介素18成熟肽片段，克隆进入PBV220载体。

The data mapping modulation, by which the information data are mapped as the combinatorial sequence, and the security protocol method of the parallel combinatory SS communication system are presented in this paper.

本文提出了并行组合扩频通信的数据调制映射法，并以此为基础，给出了一种安全通信协议方式，既可作身份认证又有好的保密能力。

Obvious genetic divergence was observed between Nepal panda and those from Yunnan and Sichuan. Additionally, DNA sequence variations were also found in concolor gibbons.

此外，对来自云南和海南的黑冠长臂猿的线粒体DNA的序列分析证实了形态和地理分布上的亚种划分，并提示海南黑长臂猿可能是一个独立的种。

The floral morphogenesis of Coptis was observed under scanning electron microscopy. The initiation of all floral organs of the genus is spiral, the petal primordia are slightly retarded, the developmental sequence of the stamens is centripetal,the carpel primordia are conduplicate (i.e., horse-shoe shaped), the ovaries are half closed and the ovary stalk is formed in the developmental process.

本文运用扫锚电子显微镜观察了黄连属植物花的形态发生和发育过程，结果表明，该属植物所有的花部器官均为螺旋状发生，雄蕊为向心式发育，花瓣原基有微弱的延迟发育，心皮原基为对折型，子房为半封闭类型，子房柄是在发育过程中形成的。

The sequence of coned NGF gene was confirmed with the dideoxychain-termination method .

以双脱氧末端终止法对克隆的 NGFcDNA 进行测序。5。

Through comparing with the results by simulation to study the effects of theprojectile"s final velocity, the angle of rotation and the ballistic trajectory"s migration withdifferent projectile"s rotating speeds, different target"s moving speeds and differentpenetration angles.

通过比较数值模拟的结果来研究不同弹头转速、目标速度、侵彻角对侵彻过程中弹头最终速度、翻转角度和弹道偏移的影响。

I love stationery and all the accoutrement of writing.

我爱文具以及所有的书写的工具装备。