查询词典 control assay

Reverse-transcription PCR assays were used to detect the mRNA expression level of related regulatory genes such as p15, p16, p21, p27, p57, surviving, cyclin B, cdc2 and chk1, and Western blot assay to detect the level of protein expression and phosphated change such as survivin, cdc2 and chk1. Results:(1) K562 cell arrested on G2/M phase were obviously increased after co-cultured with 2 to 10μmol/L Arsenic trioxide for 24 hours. The ratios of control group, 2μmol/L, 5μmol/L and 10μmol/L groups were 22.6±3.4%, 27.2±2.3%, 43.8±4.5% and 36.7±4.1%, respectively.

体外培养K562细胞，以不同浓度AS_2O_3作用不同时间后采用PI染色、流式细胞仪检测药物作用后细胞的周期分布改变；逆转录酶多聚酶链扩增方法检测细胞周期相关调节基因(p15、p16、p21、p27、p57、survivin、cyclinB、cdc2、chk1)的mRNA表达变化；Western blot方法检测细胞周期相关调节蛋白表达及磷酸化改变(survivin、cdc2、cdc2-p、chk1)。

As results, TEM showed characteristic ultrastructure morphology of apoptosis cell with chromatin marginal condense, nucleus pycnosis, mitochondrion vacuoles and cell shrinkage in IR injury group and no apoptosis cells in control group. Increased TUNEL-positive cell and typical DNA laddering were found and cells apoptotic rate were approximately 20% by flow cytometry assay in experimental groups.

值得探讨。本试验通过流式细胞仪、生物电镜、TUNEL法、RT-PCR以及DNA电泳等试验方法研究了带血供同种异体骨移植过程的细胞凋亡现象，并检测了Fas、FasL、Caspase-1、Caspase-3、Bcl-2等凋亡相关基因在IR损伤时的表达及变化情况，初步探讨了骨移植时IR损伤中细胞凋亡的发生机制及其在IR损伤中的生物学意义，希望为今后更深入的研究打下一定基础。

Experimental animals exhibited specific antibodies that were detectable 15 days after the first injection and increased steadily, the IgG2a-specific antibody titers of DNA groups were higher than IgG1-specific antibody titers, and the recombinant protein groups were contrary. The DNA vaccines elicited a T-cell-proliferative responses as well as IFN-γ production upon restimulation with the specific antigens using ELISPOT assay; the ratio of CD4+/CD8+ were lower than that of the control mice.

本研究成功构建了布鲁氏菌基因工程疫苗，在国内尚属首次，利用流式细胞仪的分型技术和ELISPOT法评价布鲁氏菌疫苗的细胞免疫指标将促进我国兽用疫苗及相关产品的实验室质控标准方法的建立和发展，为他们的临床应用奠定基础。

Methods:The level of E2 was evaluated by Progesrone Serozyme,the expression of ER was tested by Immunohistocheminal assay,ER gene expression was tested by RT-PCR in 35 cases with mammary hyperplasia of stagnation of Liver-qi type,15 cases with mammary hyperplasia of disorder of thoroughfare vessel and conception Vessel type and 30 cases of healthy people were taken as control group.

分别用磁分离酶联免疫测定法、免疫组化法和逆转录—聚合酶链反应法，检测35例肝郁气滞型乳腺增生病患者及15例冲任失调型患者和30例正常人黄体期血清雌激素水平（E2 ）、乳腺组织雌激素受体及受体基因的表达。

Seed and defatted Sesamum indicum L. were tested for two antioxidative assays, including DPPH free radical scavenging effect and Trolox equivalent antioxidant capacity assay. They were also examined for anti-aging activity using the "prolongation of C. elegans lifespan" as the model, and EUK-8 as the positive control.

再以两种评估方法检测抗氧化活性，包括清除 DPPH 自由基和 TEAC (Trolox equivalent antioxidant capacity)，另外以「线虫寿命延长」为模式，评估抗老化活性，并以 EUK-8 作为抗老化研究模式之正对照组。

Kinase activity assay method was used to estimate the activity of caspase-3 in the cells. RESULTS: Compared with control group and SOND group, in ASODN groups, the expression of survivin mRNA and protein were obviously weaken, apoptosis rate and caspase-3 activity apparently increased, cells growth was inhibited. In each ASODN group, the effect above-mentioned has time- and concentration-dependent manner.

结果： 转染ASODN各组OS-732细胞survivin mRNA和蛋白表达均明显弱于空白对照组、SODN组；细胞凋亡率显著增加，细胞生长相应受抑，caspase-3活性显著提高；上述效应在ASODN各组存在一定的时间浓度依赖性；而SODN各组与空白对照组间各项指标均无明显差异。

The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力；免疫磁珠分选技术纯化CD133+肿瘤细胞；MTT法检测CD133+细胞的体外增殖能力，并将其与CD133-及未分选细胞进行比较。

Ten normal people as a control group.Measured NO content in TMD synovia with improved fluorometric assay.

采用改良荧光分光光度法检测下颌关节液中一氧化氮含量。

A new trick using Trichogramma brassicae asvectors to transmit Dendrolimus punctatus cypovirus was developed (called"Biological missiles Dp-Ⅰ") to control the Dendrolimus punctatus in the field. Sincethen, we collect the single caterpillar from both areas used Dp-Ⅰand controlled areasevery year, and A nested-PCR-based assay for the detection of those samples wasdeveloped, The results showed that the positive reaction rate of the first year is 40～70％; and second year is 30～80％, on the other hand, the positive reaction rate of thecontrolled areas are 10～40％and 10～20％.

第二章对马尾松毛虫质型多角体病毒的流行病做了初步的调查，应用巢式RT-PCR方法检测从野外释放&生物导弹Dp-Ⅰ&的林区采集马尾松毛虫单虫样本，结果发现：2006年释放&生物导弹Dp-Ⅰ&的王福店林区的松毛虫体内DpCPV阳性率为40～70％，平均阳性率为58％，相应对照区的阳性率为10～40％，平均阳性率为24％；2005年释放&生物导弹Dp-Ⅰ&的丁家畈林区松毛虫体内DpCPV阳性率为30～80％，平均值也为58％，相应对照区的阳性率为10～20％，平均值为16％。

The has been studied. Radix isatidis, a Chinese medicinal preparation used commonly , effectively in clinical and unclarified in active substance, was chosen as a model to be studied. Based on the concept of production quality control, considering the features of TCM itself, the process of TCM preparation, especially the heating process has been studied. The physico-chemical properties assay, chemical analysis and bioassay, also the bio-thermal activity had been applied in this study.

根据药物生产质量控制理念，结合中药的特点，本文选取临床常用、疗效确切但药效物质基础不清的板蓝根为研究载体，以中药制剂过程单元操作特别是加热过程质量控制为主要着力点和切入点，在常规理化检测和化学组分分析的基础上，引入生物活性检测，探索建立基于理化—生物关联检测的中药生产过程质量控制模式和方法，以期为中药生产中原料药、半成品和成品实现全程质量控制提供技术支持。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。