查询词典 control assay

And the rats in the control were executed at 5 months to observe the develope of pancreatic cancer by macrograph and microscopy, The EnVision TM immunohistochemistry was used to assay the expression of EZH 2 and PTEN in above pancreatic specimens.

较大剂量DMBA置入胰实质内可获得较高胰腺癌发生率，TSA能抑制胰腺癌的发生和生长；EZH 2基因的激活及PTEN基因的失活在大鼠胰腺癌发生中可能起关键作用。

Methods: One hundred and twenty-six patients with UAP were randomly divided into two groups. The control group was treated with Xueshuantong injection, and the treatment group was treated with propylgallate. The clinical therapeutic effects were observed and the blood parameters such as C reactive protein, interleukin-6(IL-6) and tumor necrosis factor-α were assayed by rate nephelometry and enzyme linked immunoadsorbent assay.

选择126临床确诊为UAP的患者，随机分为血栓通注射液对照组和注射用棓丙酯治疗组，进行临床疗效观察，并分别采用速率散射比浊法及酶联免疫双抗夹心法检测C反应蛋白、白细胞介素-6(IL-6)及肿瘤坏死因子-α水平，观察其治疗前后的变化情况。

Ointment extracted from Plastrum Testudinis was separated by silica gel column chromatography, gradiently eluted by petroleum ether-ethyl acetate, and 16 (Ts-1～Ts-16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells. Results showed that Ts-12 induced proliferation compared with the control group ( p ＜0.05), while Ts-4 showed inhibitive effect. Other groups showed no significance ( p ＞0.05). GC-MS was used to analyze the chemical components in different groups.

中药龟板提取物浸膏经硅胶柱层析，用石油醚-乙酸乙酯作洗脱剂，梯度洗脱，得到16个组分；采用MTT法和流式细胞技术研究了它们对鼠骨髓间充质干细胞的增殖作用，实验结果显示组分Ts-12具有促进rMSCs增殖的作用( p ＜0.05)，而组分Ts-4则表现出抑制rMSCs增殖的作用( p ＜0.05)，其它样品对rMSCs的增殖作用不显著( p ＞0.05)，不具统计学意义。

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞，并在有或无美洲商陆有丝分裂原存在的情况下培养10天后，收集上清液，采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

The fungal control spectrum of α-pyrone 0.2% WP was investigated using a mycelial growth rate assay. PDA culture medium containing 20 μg/ml of the pesticide inhibited all of the 11 pathogenic fungi tested.

采用菌丝速率法测定了0.2%α-吡喃酮WP的抗菌谱，含药浓度20μg/ml的PDA培养基对测试的11种植物病原真菌均有抑制作用，其中对禾谷丝核菌的抑制作用最强，抑制率为89.3%。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Ribonuclease protection assay ; kit ; quality control

核糖核酸酶保护分析；试剂盒；质量检测

Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件，最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1：25倍稀释、最佳反应时间为2h；与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应；阳性样品经64倍稀释后依然可检出，检测灵敏度是传统微孔板ELISA的4倍。

ACA was detected in the sera of 176 patients and 98 members in control group by enzyme linked immunosorbent assay.

用酶联免疫吸附试验对176例急性脑血管病患者和98例健康对照者进行ACA检测。

And cell differentiation was assayed by measuring the cellular alkaline phosphatase activity with dexamethasone as positive control. The influence of ethanol and dimethyl sulfoxide on cell proliferation and differentiation assay was also investigated.

成骨样细胞的分化采用测定细胞内碱性磷酸酶活性的方法，地塞米松为阳性对照药，同时考察了溶剂乙醇和二甲基亚砜对细胞增殖和分化的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。