查询词典 control assay

Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group（group A, n = 10）, diabetic group for 4 weeks（group B, n = 14）, and diabetic group for 12 weeks（group C, n = 16）. The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组：A组为正常对照组（10只），B组为糖尿病4周组（14只），C组为糖尿病12周组（16只），采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后，A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能，并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达，同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组：A组为正常对照组（10只），B组为糖尿病4周组（14只），C组为糖尿病12周组（16只），采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后，A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能，并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达，同时采用电泳迁移率变动分析法进行NF-κB活性检测。

To assay the activity of matrix metalloproteinases in the aqueous humor by the same way in four groups of albino rabbit eyes treated with Lumigan for 3, 7, 14, 21 days respectively and the control group treated with normal saline.

用同样的方法检测滴用Bimatoprost 3、7、14、21天后兔眼房水中MMPs的变化，并应用UVI凝胶电泳定量分析系统进行各个条带的定量分析。

This system is divided three modules: the section office of operation, the section office of assay and the section office of quality control.

该系统分为三个模块：业务科，检验科和质量控制科。

Methods HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs.

采用组织块培养法培养原代人牙周膜成纤维细胞并传代，经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定，取5~8代细胞用于实验；按茶多酚不同浓度分1mg/ml、0.5mg/ml、0.25mg/ml、0.125mg/ml、0.0625mg/ml组和空白对照组，采用细胞计数法、MTT法检测细胞增殖情况，流式细胞术检测细胞DNA含量。

Methods 60 patients with newly diagnosed ITP, their serum TPO concentration were detected by double-antibody sandwich enzyme-linked immunosorbent assay; and the bone Marrow megakaryocytes were recorded; HP was detected by 14C urea breath test. The control group of 40 cases was also detected by above methods.

60例初治ITP患者，在接收治疗前采用双抗体夹心酶联免疫吸附法检测血清TPO水平，计数骨髓涂片的平均巨核细胞数量，用14C尿素呼气试验检测患者HP感染情况。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group.Immune indexes were examined by using immune staphytococcal protein A rosette test,indirect enzyme-linked immunosorbent assay,cellular culture technique and MTT method.

将180只14日龄雏鸡随机分为毒害艾美球虫初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。

The endermic LD50 to rat and mouse were 926 mg/Kg and 1210 mg/Kg respectively. The results of subscute toxicity test in rats showed that the content of blood urea nitrogen in exposure groups was higher than that in control group, the histopathological changes of testis observed in exposure groups were obvious. Mutagenic tests including bacillus subtilis DNA rec-assay, micronucleus ...

其大、小鼠经皮LD_(50)分别为926mg/kg和1210mg/kg；大鼠90天经皮染毒，试验组BUN含量高于对照组，光镜下，试验组睾丸的组织学结构有明显改变；枯草杆菌DNA重组试验、小鼠骨髓多染红细胞微核试验和小鼠睾丸染色体畸变分析试验均获得阳性结果。

The in vitro antifungal assay provided scientific basis for applying GAFP to genetic engineering to prevent and control plant fungous diseases in the near future.

离体抑菌谱的测定为用基因工程的方法防治这些植物真菌病害提供了科学的实验依据。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。