查询词典 control assay

The results were confirmed by virus isolation. The RNA positive control in the assay was invitro transcribed dsRNA which was stabler than single strand RNA used in other similar test.

本研究建立的检测方法以体外转录的dsRNA为阳性对照，具有高度的稳定性，解决了类似检测方法中RNA阳性对照易降解的难题。

Methods The serum p53 antibody level was detected in 120 patients with lung cancer, and in 30 patients with benign pulmonary lesion and 120 healthy adults as control by enzyme linked immunosorbent assay. The blood samples were collected on the day before operation and on the 7th, 30th and 90th days postoperatively.

采用酶联免疫吸附法分别于术前1天、术后7、30、90天动态检测120例肺癌患者血清p53抗体，并以30例肺部良性疾病患者和120例正常健康人作对照。

MethodsThe levels of CEA,CA199 and CA242 in serum and gastric fluid that gathered from 28 cases of gastric cancer and 32 cases of precancer were analyzed by chemiluminescence immunoassay and enzyme linked lmmunosorbant assay, which control by 28 healthy.

方法采用化学发光和ELISA法测定28例胃癌、32例癌前疾病患者和28例对照者血清及胃液中CEA、CA199、CA242的含量。

The SNPs were genotyped by homogenous mass extend assay in nondiabetic control population.

根据所发现的单核苷酸多态性，通过同源引物延伸质谱分析法对非糖尿病对照人群进行基因分型。

Objective: the correlative study of the expression of MMP-9, E-cd and PTEN in glioma. Methods: the expression of MMP-9, E-cd and PTEN were examined in 50 patients with glioma and 11 cases of control brain tissues by immunohistochemical assay.

目的：探讨基质金属蛋白酶-9(Matrix Metalloproteinases-9、MMP-9)，钙粘素(E-cadherin、E-cd)和PTEN在脑神经胶质瘤中的表达意义，并做出相关性分析。

The protein expression level of Syk was lower than the control by Western Blot assay,but that of IFN-γwas higher.

Western Blot提示：试验组SYK蛋白表达降低，而IFN-γ蛋白表达增高。

after treated with glu (5 mmol/l) for 24 h, the viability of pc12 cells depressed and the viable cells was only 58.3%when compared with cell control group tested by mtt assay.

体外培养pc12细胞，建立谷氨酸诱导pc12细胞损伤的模型；采用mtt法检测细胞活力，流式细胞术和hoechst 33258 dna 染色法检测细胞凋亡。

METHODS: Fasting serum levels of IL6, IL8, TNFα were measured in 30 cases of T2DM with insulin resistance and 30 cases of T2DM without insulin resistance and 26 normal control subjects by enzymelinked immunosorbent assay.

采用酶联免疫吸附法测定T2DM伴有IR患者30例和不伴有IR患者30例及正常对照26例的空腹血清白细胞介素6（IL6），白细胞介素8（IL8）及肿瘤坏死因子的浓度，并同时测定空腹血糖、糖化血红蛋白（GHbA1c）及空腹胰岛素水平，分析其与IL6，IL8，TNFα的相关性。

The reasons for using E.coli system to express HCV non-structure proteins are as follow:(1) Hepatitis C virus infections are serious in China. Using anti-HCV screening assay to control the quality of blood is the main measure of preventing the transmission of HCV.

本研究利用大肠杆菌系统表达丙型肝炎病毒非结构蛋白主要是基于以下考虑：(1)丙型肝炎在我国流行严重，利用HCV检测试剂控制血源质量是现阶段的主要预防措施。

Control group was also set up.Then the AO/EB fluorescence double-dye technique,immunocytochemistry staining,Fluo-3 staining and Rhodamine 123 uptake assay approaches were adopted to observe the apoptotic rate,expression levels of Bcl-2 and Bax proteins,concentration of cytoplasmic free Ca2+ and mitochondrial transmembrane potential of NCI-H460 cells.

采用AO/EB荧光双染、免疫细胞化学染色、Rhodamine 123摄取法及Fluo-3/AM染色分别观察NCI-H460细胞的凋亡、Bcl-2及Bax蛋白的表达量、线粒体跨膜电位及细胞内游离钙离子浓度。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。