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The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

In the same time, water-soluble bidental phosphines ligand BISBIS sulfonated 2,2''-bis(diphenylphosphinomethyl-1,1''-biphenyl was synthesized, and water-soluble rhodium complex Cl was synthesized in situ. This complex which was used as catalysts to catalyze the hydrogenation of avermectin was also tested. Although the rhodium complex containing bidentate phosphine BISBIS showed a higher catalytic activity than one containing TPPDS, the selectivity for ivermectin was lower. How to improve the selectivity will be needed to studied in futur.

同时我们用磺化的2，2''-二-- 1，1''--联苯，以及它们的水溶性威尔金森型铑配合物和环辛二烯的阳离子铑配合物，在利用这些配合物作催化剂，考察它们在各种反应条件下对阿维菌素的选择加氢，由于反应条件以及复合催化剂体系的组成还需要进一步优化，虽然双齿膦配合物显示了较高的活性，然而选择性还有待进一步提高。

The invention comprises a cyclopalladated catalyst with axial chiral binaphthyl backbone containing phosphine ligand as well as the synthesis thereof, and the application of the catalyst with cyclopalladated catalyst with axial chiral binaphthyl backbone containing phosphine ligand with a similar structure to the hetero atom norbornadiene ring cleavage reaction through an asymmetric catalytic organic boron agent.

本发明包括一类具有轴手性联萘骨架含膦配体的环钯催化剂及其合成，以及其与具有类似结构的轴手性联萘骨架含膦配体的环钯催化剂在不对称催化有机硼试剂对杂原子降冰片二烯开环的应用。

Research progresses in the oganoboron π-conjugated materials are reviewed in this paper, in terms of the molecular structures of the π-conjugated systems, including the linear π-conjugated systems with boryl groups at the terminal positions, the starburst π-conjugated systems containing boron atoms at the center, the polymeric π-conjugated systems containing boron atoms in the main chain, the boron-bridged ring-fused π-conjugated systems, as well as the π-conjugated systems with boryl groups at the side positions.

本文根据π-共轭体系分子结构的不同，对有机硼π-共轭材料，包括硼取代基位于末端的线形π-共轭体系、硼原子位于中心的星形π-共轭体系、硼原子位于主链的π-共轭聚合物体系、硼原子桥联的并环π-共轭体系以及硼取代基位于侧链的π-共轭体系等的研究进展进行了综述，并进一步对该领域的发展前景进行展望。

A process for the carbonylation of allylic butenyl ether (for example, methyl crotyl ether, 3-methoxybutene-1 and mixtures thereof) or mixture of butadiene and alcohol and production of beta-gamma unsaturated carboxylic acid esters (for example, methyl-3-pentenoate) utilizing a rhodium-containing catalyst for example, dicarbonylacetylacetonate rhodium(I or the like promoted with an iodide-containing compound (for example, HI, AlI3, SnI4, TiI4, CrI3, and CoI2 or the like).

一种将烯丙型丁烯基醚(如甲基巴豆基醚、3－甲氧基丁烯－1及其混合物)或者丁二烯与醇的混合物羰基化的方法，以及由含碘化物化合物(如HI、AlI3、SnI4、TiI4、CrI3和CoI2或等效物)促进，用含铑催化剂如二羰基乙酰丙酮酸铑(I或其等效物制备β－γ不饱和羧酸酯(如甲基－3－戊烯酸酯)的方法。

The purpose of this study was to investigate the effect of different doses o f dietary fiber of Tremella fuciformis Berk on lipid metabolism and inte stinal physiology in rats fed diets containing 1% cholesterol and 0.2% cholica cid. Forty-two male Wistar rats were randomly divided into six groups in a 2× 3 factorial design and were fed diets containing different doses (0%、5% or 10 %) of dietary fiber of TFB, with and without antibiotics 0.7g/100g of Nebaci tin (bacitracin: neomycin sulfate = 2:1,w/w) for 4 weeks. The parameters analyz ed included liver and serum lipid concentration , serum and caecal content of short chain fatty acids ,fecal neutral steroids and total bile acids content.T he results showed that rats fed the diet with antibiotics had lower levels ofs hort chain fatty acids in serum and caecal content than rats fed diet withouta ntibiotics.

中文摘要本研究乃欲探讨在有高胆固醇饮食条件下，摄取不同量的白木耳膳食纤维及添加抗生素对大白鼠脂质代谢及肠道生理的影响。42只 Wistar 品系雄鼠(重约260g)，随机分为六组，每组饲料中均添加 1%胆固醇及 0.2%胆酸，并以添加或不添加抗生素[ 0.7g Nebacitin(bacitracin：neomycin sulfate=2：1，w/w)/100g dry matter] 、白木耳膳食纤维量(0%、5%或 10%)作 2×3 factorial 之实验设计，实验期四周，分析血清及肝脏中脂质浓度，血清及盲肠内容物短链脂肪酸浓度，以及粪便中总中性固醇，总胆酸的含量。

With review of the role of Ti-containing catalysts in the modern catalytical industry and its impetus to the development of modern industry,the paper briefly describes the latest development of Ti- containing catalysts.

在概述含钛催化剂于现代工业催化剂中的地位和对现代化工的发展所起推动作用的基础上，就近年含钛催化剂研究的新进展及我国催化科学工作者所做的相关研究和有关文献作了简要介绍。

Various mole ratios of acrylonitrile and methyl methacrylate were used to copolymerize in aqueous solution by Redox and the copolymers were obtained. The copolymers containing MOCT were synthesized by adding MOCT before copolymerization. The copolymer were derived from the copolymerization of AN and MMA in 30L kettle and then were mixed with various contents of MOCT, the copolymer films were cast by solution of the copolymer containing MOCT.

本课题采用不同摩尔比的丙烯腈和甲基丙烯酸甲酯进行水相沉淀聚合，制备了AN／MMA共聚物；在聚合阶段加入正十八烷微胶囊，制得了正十八烷微胶囊含量不同的共聚物；选择合适的条件进行大釜聚合，得到共聚物后与不同比例的正十八烷微胶囊混合，通过流延制得正十八烷微胶囊含量不同的共聚物膜；并尝试进行了纺丝实验。

Said chemical matter comprises compound containing ammonium and halogen atom absorption, and lower carbon number hydrocarbons containing halogen atom and it's derivant; it can be used separately or together with urea, ammonia gas and natural gas.

这些化学添加物包括含有铵和卤原子的化合物、含有胺及卤原子的化合物以及含有卤原子的低碳烃及其衍生物，可以单独使用或与尿素、氨、天然气等主还原剂混合使用。

Accordingly, a new plasmid Cm1301 was constructed containing osGP fragment (-197～-56) from the osRACD promoter with the minimal promoter,-90 region of CaMV 35S which confers Gus activity detectably only in roots. In the transgenic plants containing Cm1301, GUS activity was detected mostly in the anther.

由于35S基本启动子是一个根特异表达的启动子，因此将包含osDP5区段的osGP片段（-197～-56）与35S基本启动子连接，构成植物表达载体Cm-1301并转化烟草，研究发现，osGP区段使gus基因在雄蕊中表达水平显著提高。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了