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After discussing the structural conditions of the existence of the active network solutions, the state equations in normal form over F of electrical network which are suited for studying the character of network structure are derived. If there is no loop containing only capacitor and independent voltage sources, and if there is no cut-set containing only inductors and independent current-sources, both matrix A and B in ?

在探讨了有源网络解存在的结构条件下,推导了适合研究网络结构特征的多元有理函数域上电网络的范式状态方程:如果不存在仅由独立电压源和电容器构成的回路,且不存在仅由独立电流源和电感器组成的割集,则无论是有源网络还是无源网络,在网络的范式状态方程?

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统:运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞:用含有400ug/mlG418的F12培养基培养14天,筛选出稳定阳性表达克隆,RPMI1640培养基扩增获得稳定表达细胞株,并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02,表达率分别为53.7%、54.5%;应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株;RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株,裂解细胞得到CD59蛋白质;通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达;50mM核糖培养72小时,获得突变人CD59糖化细胞株,BCECF染料释放试验结果显示,PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低,未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The iron nanoparticles in inner core have good magnetism and targeting heating effect. The temperature can go up to 52 ℃ in the case that 0.1 g carrier (carbon-coated iron nanoparticles) is injected in a local point of pig's liver,while the temperature can go up to 42℃ in the case that pig's liver uniformly containing carrier of 0.4 wt.% and 45℃ in the case that pig's liver uniformly containing carrier of 0.6 wt.%. Carbon-coated iron nanoparticles also has good antioxidation and stability by an X-rays diffraction structural analysis.

铁纳米粒子磁场强,靶向效果好,有较佳的磁靶向发热效应,在动物病灶局部放置0.1 g碳包铁纳米粒子将能使发热温度平均升到52℃;而将碳包铁纳米粒子均匀与猪肝混合也有明显的产热效果:含量0.4%碳包铁纳米粒子的一组猪肝能将温度升到42℃,而含量0.6%碳包铁纳米粒子的一组猪肝能将温度升到48℃。X射线衍射表明借助于碳的包裹,碳包铁纳米粒子有好的抗氧化性和稳定性。

The role of the reclaimed rubber: smoke sticky pine tar containing other renewable agent, during which small molecules can penetrate into uniform knot so that sulfur containing rubber between macromolecules to swelling, reticular structure of relaxation, an increase of molecular the distance between chain and oxygen penetration, the other can also serve as free radical acceptor, has inhibited the role of gelation, but also contribute to activating agent to the rubber matrix to speed up the regeneration process of proliferation and can enhance regeneration and plasticity of sticky glue and less polluting, process performance.

对再生胶的作用:粘稠的松焦油吸含其他的再生剂,其间的小分子能均匀渗透到含有结使硫的橡胶的大分子中间,使其溶胀,网状结构松驰,增加了分子链间的距离及氧的渗透,另外还可作为游离基的接受体,有抑制凝胶化的作用,还有助于活化剂向橡胶基质扩散加快再生过程,并能提高再生胶的粘性和可塑性,且污染较小,工艺性能好。

The preparation process of self-holing polyimide with organosilicon block includes the following steps: 1 the first reaction of polyacid anhydride in functionality not lower than 2, silicon oil with 2 or more amino or imino groups and polyamine with 2 or more amino groups, and the second reaction with one or several kinds of added isocyanate with 2 or more isocyanate radicals to produce sol of polyamido acid containing organosilicon block; 2 solidifying the sol to obtain solid polyamido acid containing organosilicon block; and 3 imidonating the solid to obtain self-holing polyimide with organosilicon block.

本发明公开了一种有机硅嵌段自发孔聚酰亚胺的制备方法,该方法包括以下步骤:(1)有机溶剂中具有大于或者等于2的酸酐官能团度的多酸酐与具有大于或者等于2的胺基或亚胺基的硅油以及具有大于或者等于2的胺基的多胺反应,随后添加一种或多种具有含有等于或者大于2个异氰酸酯基的异氰酸酯反应,生成含有机硅嵌段聚酰胺酸的溶胶。(2)溶胶固化制成有机硅嵌段聚酰胺酸固体。(3)固体亚胺化,即得有机硅嵌段自发孔聚酰亚胺。

On the 10th ,20th ,30th,40th and 50th day, cut the tail of each mouse to make blood smears, stained the smears by Wrights method, and counted the erythrocyte containing HowellJolly bodies, or containing basophilic stipplings, or showing polychromatophilia, or showing hypochromic.

昆明种成年雄性小鼠15只,随机分成两组,实验组10只饮用2g/L硝酸铅溶液(1251mgPb2+/L)染毒,共50d。每10d切尾涂片一次,分别镜检计数含HowellJolly小体RBC,嗜碱性点彩RBC,嗜多色性RBC及低色素性RBC的出现率。

According to the invention, a base polymer which is produced on the basis of a monomer including up to at least 50 % neutralised acid groups is mixed with a first aqueous solution containing at least one surface secondary crosslinker and with a second aqueous solution containing at least one polyvalent cation, and is thermally treated.

根据本发明,使基质聚合物与含有至少一种表面二次交联剂的第一水溶液和含有至少一种多价阳离子的第二水溶液混合,并热处理,其中所述基质聚合物基于含有至少50%中和的酸官能团的单体而制备。

It includes three new chapters on ferrocene-based polymers, other phosphorous-containing polymers, and boron containing polymers; inorganic-organic hybrid composites; and preceramic inorganic polymers and provides a useful reference for both professionals and students.

它在基於ferrocene的聚合物,其他亚磷包含的聚合物和硼包含聚合物上包括3 新章;无生物有机的混合的混合物;并且无生物的preceramic 聚合物和提供有用参考适合专业人士和学生。

The contraceptives containing both the hormones are more effective than that of containing progestin only.

避孕药含的激素是更有效地比只含孕。

Containing a greater proportion of alcohol than proof spirit, especially containing more than50 percent alcohol by volume.

超标准酒精度的所含酒精超过标准度数的,尤指含酒精容量超过50%的

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在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

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