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We successfully identified JSRV mRNA expression and proviruses DNA in sheep lung tissues infected with JSRV and control group has no postive signals,Validating the use of exogenous virus-specific DNA probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences.

用地高辛随机引物法标记exJSRV特异的env片段，制备探针，原位杂交检测SPA肺组织中的RNA及前病毒DNA，结果表明SPA患羊肺组织内有JSRVenv基因mRNA的表达，同时也检测到了前病毒DNA，而相应的阴性对照却无阳性信号，证实外源性病毒特异的DNA探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

The six groups within Enraf are: Enraf Terminal Automation (precision measurement systems and software for tank storage companies), Enraf Calibron Systems (small volume provers and liquid density meters), Enraf Contrec Ltd.(fuel Management Systems used by the petrochemical industry at bulk tank storage sites), Enraf Fluid Technology (custom-engineered explosion-proof precision blending and additive metering equipment), Enraf Marine Systems (level measurement systems for marine applications) and Enraf Tanksystem SA (mobile precision level measurement systems used on board ships).

Enraf控股的六个集团为： Enraf 终端自动化（用于油罐存储公司的精密测量系统和软件）、 Enraf Calibron系统（小量校准仪和液体密度仪）、Enraf Contrec公司（用于石化工业大量油罐存储场的燃料管理系统）、Enraf 射流技术（工程定制防爆精密混合和添加计量设备）、Enraf 航海系统（航海应用的水平面测量系统）以及Enraf Tanksystem SA（用于船上的移动精密水平面测量系统）。

The sequence analysis indicated that the integrate sites of prophages 933v and 933w Located at 2966137 and 1330826, respectively.

结果筛选到了原噬菌体消除后的O157菌株，该菌株丧失了对Vero细胞的毒性作用。

There were 20bp and 7bp direct repeat sequences flanking the prophages 933v and 933w. The flanking repeat sequences are termed as attachment sites.

序列比较分析表明原噬菌体933v的整合位点位于O157：H7 EDL933基因组中第2966137位的相对位置，在整合位点两侧有一个20bp的直接重复序列，原噬菌体933w的整位点位于基因组中第1330826位的相对位置，在整合位点两侧有一个7bp的直接重复序列。

Transcription factors can control the transcription of their target genes when they bind to the DNA-binding sites on their promotors.

转录因子是一类可以通过与下游靶DNA结合，从而调控下游基因转录的分子，它们接受来自生长因子等方面的信号，再将这些指令具体地传递给直接执行功能的蛋白分子。

Transcription factors can control the transcription of their target genes when they bind to the DNA-binding sites on their promotors. They can transfer the messages of growth factors and so on to those effective molecules. Core binding factor al is such a kind of transcription factor, which can control the differentiation of osteoblasts peculiarly. They can induce the pro-osteoblasts or non-osteoblasts to synthesize and secrete most mineralize related proteins such as ALP, OC, OPN, BSP and so on, in order to differentiate to osteoblasts finally.

转录因子是一类可以通过与下游靶DNA结合，从而调控下游基因转录的分子，它们接受来自生长因子等方面的信号，再将这些指令具体地传递给直接执行功能的蛋白分子。cbfal是一种成骨分化特异性转录因子，可以诱导成骨前体细胞合成和分泌ALP、OC、OPN、BSP等矿化相关蛋白，向成骨细胞分化。

More important still, though, is that people can use a mobile phone to build their own tibia platinum voice sites—a process that, in trials conducted by the laboratory, even a non-expert could learn in as little as ten minutes.

除此之外，更令人惊奇的是，人们还可以通过移动电话搭建属于自己的语音网站，当然，目前这一项目正处于实验室试行阶段，如果成功，那么一个网站外行就能在10分钟内学会搭建网站。

These sites are trading under false pretences.

这些地点都是虚假的理由营运。

These sites are trading under false pretences.

这些土地交易下瞒天过海。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"